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This review addresses challenges in scaling iPSC-derived therapies, emphasizing automation, closed systems, and Quality-by-Design. It evaluates various technologies for expansion and differentiation. 1L and 5L G-Rex are referenced as technologies used for expansion during research and early process development.

This study establishes a GMP-compliant protocol for expanding thymus-derived regulatory T cells (Thy-Tregs). By optimizing CD8 depletion, CD25 enrichment, and cytokine stimulation, the authors achieved high purity. G-Rex (G-Rex 10 and 100) were successfully used to scale up production for clinical trials.

This study analyzes adaptive immune responses in PSC. Cells were pulsed with peptides in a 24-well G-Rex device to generate EBV-specific T cell lines.

RAPA-201 T cells, reprogrammed via mTOR inhibition, showed safety and efficacy in RRMM patients. The manufacturing process involved incubating cells in G-REX culture vessels with cytokines and rapamycin.

The authors demonstrate that DNA-PK inhibitors enhance CRISPR/Cas9 KI efficiency. Under GMP-optimized conditions, T cells were cultured in G-Rex-100M vessels and seeded in G-Rex 24 well plates after electroporation.

The study evaluates Pin-EGFR-armed eNK cells. CD3-negative cells were seeded into G-Rex 6M devices for expansion and transferred to 100 M G-Rex on day 7.

The NEO-STIM process generates high-frequency T cell responses to KRAS neoantigens. The protocol utilizes G-Rex 24 Well Plates, G-Rex 6 Well Plates, and G-Rex 100M/500M closed-system vessels for cell culture and expansion.

The study identifies viral exmiRs as markers for live virus infection in T cells. Purified T cells were activated and cultured in a G-Rex 24-well plate.

This study profiles CAR T cells across manufacturing. Transductions and expansion were carried out in G-Rex 6M vessels to support high-density cell growth.

The authors developed an efficient protocol for expanding CAR-T and CIK cells using G-Rex 24 and G-Rex 6M devices. The study highlights the scalability and high cell yield obtained using these gas-permeable culture systems.

The study reports the optimized GMP-grade manufacturing of TranspoCART19 cells. Following electroporation, cells were expanded for 11 days in G-Rex 100M devices, achieving high expansion and functionality.

This study develops AI models to quantify TILs in colorectal cancer liver metastasis. In the Rapid Expansion Protocol (REP) stage, TILs were cultured in G-Rex 100 M.