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The 4-1BBζ costimulatory domain in chimeric antigen receptors enhances CD8+ T-cell functionality following T-cell receptor stimulation

This study demonstrates that the 4-1BBζ costimulatory domain enhances CD8+ T-cell proliferation and cytokine production following TCR stimulation compared to CD28ζ. CAR T-cells were manufactured and expanded in G-Rex plates for 13 days prior to functional analysis.
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Publications

Expansion of tumor-infiltrating lymphocytes from head and neck squamous cell carcinoma to assess the potential of adoptive cell therapy

This study assesses TIL expansion from HNSCC samples, finding sTIL percentage as a key predictor. A subset of samples underwent a Rapid Expansion Protocol (REP) using either T-flasks or G-Rex vessels, achieving high fold expansion.
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Publications

Manufacturing CD20/CD19-targeted iCasp9 regulatable CAR-TSCM cells using a Quantum pBac-based CAR-T engineering system

This paper describes a virus-free CAR-T manufacturing process using the Quantum pBac system and Quantum Booster supplement. Use of G-Rex 24-well and 1L vessels allowed for the generation of clinically relevant numbers of high-quality CAR-TSCM cells in 10 days.
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Publications

Stem cell-derived CAR T cells show greater persistence, trafficking, and viral control compared to ex vivo transduced CAR T cells

The study demonstrates that HSPC-derived CAR-T cells exhibit superior persistence and viral control compared to adoptively transferred T cells in HIV models. The adoptive CAR-T cell products were manufactured and expanded for ~7 days in G-Rex.
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Publications

Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence

This study shows that modulating glucose/glutamine during activation and expansion (metabolic priming) improves TRAC-CAR T cell stemness and in vivo persistence. Expansion of the gene-edited cells was performed in G-Rex 6M plates.
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Publications

Bispecific CD33/CD123 targeted chimeric antigen receptor T cells for the treatment of acute myeloid leukemia

The researchers designed a split-signaling bispecific CAR to target AML cells while sparing HSCs. CAR T cells were expanded using G-REX plates to generate sufficient cell numbers for in vivo xenograft experiments comparing bispecific and monospecific CARs.
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Publications

Pretreatment with IL-15 and IL-18 rescues natural killer cells from granzyme B-mediated apoptosis after cryopreservation

This study identifies that GZMB leakage causes apoptosis in cryopreserved NK cells and that IL-15/IL-18 pretreatment mitigates this. NK cells were isolated and expanded for 15-20 days in G-Rex 6-well plates using irradiated K562 feeder cells.
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Publications

Modular chimaeric cytokine receptors with leucine zipper enhance the antitumor activity of CAR T cells via JAK/STAT signalling

The authors developed leucine zipper-based receptors (ZipRs) to mimic cytokine signaling in CAR-T cells, enhancing their function. Autonomous cell outgrowth assays were conducted by seeding ZipR-transduced T cells in G-REX 6-well plates to ensure safety.
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Publications

CD8α Structural Domains Enhance GUCY2C CAR-T Cell Efficacy

This paper demonstrates that the CD8α hinge/transmembrane domain improves GUCY2C CAR-T cell affinity and antitumor efficacy compared to CD28 domains. CAR-T cells were expanded ex vivo for 12 days using G-Rex 6-well plates prior to cryopreservation.
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Publications

HER2 and HLA- A*02 dual CAR- T cells utilize LOH in a NOT logic gate to address on- target off- tumor toxicity

The study presents a logic-gated CAR-T strategy to safely target HER2 by sparing normal HLA-A*02+ cells. G-Rex 24 or 6-well plates were utilized for the expansion of CAR-T cells after lentiviral transduction, supporting the generation of cells for in vitro and in vivo assays.
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Publications

Silicone rubber membrane devices permit islet culture at high density without adverse effects

This study demonstrates that G-Rex devices allow for a 20-fold increase in islet seeding density compared to T-flasks without compromising viability or function. Modeling and experiments confirm that the gas-permeable membrane prevents anoxia in high-density cultures.
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Publications

Drug-regulated CD33-targeted CAR T cells control AML using clinically optimized rapamycin dosing

Describes DARIC33, a CD33-targeted CAR-T controlled by rapamycin to mitigate AML toxicity. Clinical-scale T cell products were manufactured using G-Rex vessels, demonstrating process feasibility and producing cells with high engraftment potential.
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