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This study investigates the specificity of the MR1-restricted TCR from clone MC.7.G5. Results show recognition is restricted to the MR1*04 allomorph and is not cancer-specific, reacting to healthy cells expressing MR1*04. G-Rex systems were used to seed and expand CD3+ T cells during the transduction process.

This study demonstrates that the 4-1BBζ costimulatory domain enhances CD8+ T-cell proliferation and cytokine production following TCR stimulation compared to CD28ζ. CAR T-cells were manufactured and expanded in G-Rex plates for 13 days prior to functional analysis.

This study assesses TIL expansion from HNSCC samples, finding sTIL percentage as a key predictor. A subset of samples underwent a Rapid Expansion Protocol (REP) using either T-flasks or G-Rex vessels, achieving high fold expansion.

This paper describes a virus-free CAR-T manufacturing process using the Quantum pBac system and Quantum Booster supplement. Use of G-Rex 24-well and 1L vessels allowed for the generation of clinically relevant numbers of high-quality CAR-TSCM cells in 10 days.

The study demonstrates that HSPC-derived CAR-T cells exhibit superior persistence and viral control compared to adoptively transferred T cells in HIV models. The adoptive CAR-T cell products were manufactured and expanded for ~7 days in G-Rex.

This study describes 'metabolic priming' of GD2 TRAC-CAR T cells during manufacturing by activating them in glucose/glutamine-low medium and expanding in glucose/glutamine-high medium. This process enhances stem-like memory phenotypes and in vivo persistence. G-Rex6M plates are referenced for ex vivo cell expansion.

The researchers designed a split-signaling bispecific CAR to target AML cells while sparing HSCs. CAR T cells were expanded using G-REX plates to generate sufficient cell numbers for in vivo xenograft experiments comparing bispecific and monospecific CARs.

This study identifies that GZMB leakage causes apoptosis in cryopreserved NK cells and that IL-15/IL-18 pretreatment mitigates this. NK cells were isolated and expanded for 15-20 days in G-Rex 6-well plates using irradiated K562 feeder cells.

The authors developed leucine zipper-based receptors (ZipRs) to mimic cytokine signaling in CAR-T cells, enhancing their function. Autonomous cell outgrowth assays were conducted by seeding ZipR-transduced T cells in G-REX 6-well plates to ensure safety.

This paper demonstrates that the CD8α hinge/transmembrane domain improves GUCY2C CAR-T cell affinity and antitumor efficacy compared to CD28 domains. CAR-T cells were expanded ex vivo for 12 days using G-Rex 6-well plates prior to cryopreservation.

The study presents a logic-gated CAR-T strategy to safely target HER2 by sparing normal HLA-A*02+ cells. G-Rex 24 or 6-well plates were utilized for the expansion of CAR-T cells after lentiviral transduction, supporting the generation of cells for in vitro and in vivo assays.

This study demonstrates that G-Rex devices allow for a 20-fold increase in islet seeding density compared to T-flasks without compromising viability or function. Modeling and experiments confirm that the gas-permeable membrane prevents anoxia in high-density cultures.