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Publications

Context-specific synthetic T cell promoters from assembled transcriptional elements

The study creates synthetic promoters for CAR-T cells. T cells nucleofected with piggyBac transposase were transferred to G-REX culture vessels for 21 days of expansion.
Publications

Preparation of cryopreserved chimeric antigen receptor T cells for the locoreogional delivery to the neural axis

Describes a thaw/wash method for CAR-T cells for intracranial delivery. B7-H3-CAR T cells were generated and expanded in G-Rex culture devices using X-VIVO 15 media with cytokines.
Publications

Vγ9Vδ2 T cells expressing a BCMA—Specific chimeric antigen receptor inhibit multiple myeloma xenograft growth

This study generates BCMA-CAR Vg9Vd2 T cells via mRNA electroporation. Vg9Vd2 T cells were expanded using Zometa and co-cultured with aAPCs. Previous work by the group used G-Rex vessels for large-scale expansion.
Publications

Humanized CD19-directed CAR-T Cell Therapy in Pediatric Relapsed/Refractory Acute Lymphoblastic Leukemia With CNSL or Neurological Comorbidity

This clinical study evaluates humanized CD19 CAR-T cells in pediatric R/R ALL with CNS issues. Transduced T cells were cultured, amplified, and harvested over 5–7 days using the G-Rex system.
Publications

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Development of BAFF ligand-based CAR-T cells targeting BAFF-R, BCMA, and TACI. Cells were modified using the TcBuster transposon system and maintained in G-Rex culture vessels.
Publications

Feasibility and preclinical efficacy of CD7-unedited CD7 CAR T cells for T cell malignancies

The study demonstrates manufacturing of CD7 CAR T cells using ibrutinib/dasatinib to prevent fratricide. cGMP manufacturing involved transduction and expansion in G-Rex culture devices for 3 additional days.
Publications

Chimeric antigen receptor structure and manufacturing of clinical grade CAR engineered cells using different bioreactors

This review discusses CAR structure and automated manufacturing platforms. It highlights the G-Rex (M series) and GatheRex pump as a practical, cost-effective, and scalable static culture option for CAR-T expansion.
Publications

Improving cytokine-induced killer cell expansion using a gas-permeable culture method for clinical-scale production

This paper compares CIK cell expansion in G-Rex devices vs standard plates. G-Rex 24-well and 6-well plates were used. G-Rex resulted in significantly higher expansion and IFN-g secretion, supporting clinical-scale production.
Publications

Salt- inducible kinase 3 protects tumor cells from cytotoxic T- cell attack by promoting TNF- induced NF- κB activation

The study identifies SIK3 as a gene protecting tumor cells from T-cell cytotoxicity. In the methods, TILs were rapidly expanded using a modified Rosenberg protocol involving culture in G-Rex 100 with feeder cells and IL-2.
Publications

Adaptive single- KIR+NKG2C+ NK cells expanded from select superdonors show potent missing- self reactivity and efficiently control HLA- mismatched acute myeloid leukemia

This study describes the ADAPT-NK cell product derived from superdonors. The expansion protocol involved co-culturing CD3/CD19-depleted PBMCs with feeder cells in G-Rex 24 plates for 11 days to generate high numbers of adaptive NK cells.
Publications

Improving multiTAA-Specific T Cell Phenotype and Potency Through Process Evolution

The study outlines improvements to the manufacturing of MT-401 (multiTAA-specific T cells). The new process using G-Rex 10M and scaling to G-Rex 500M reduced duration to 9 days and improved T cell purity and memory phenotype.
Publications

in vitro 3D Tumor Models using the Go-Rex Platform

This poster introduces the Go-Rex device, built on G-Rex technology, for long-term 3D tumor modeling. It allows assessment of T-cell migration, infiltration, and killing over extended periods compared to traditional assays.

ScaleReady is a joint venture between Wilson Wolf Manufacturing and Bio-Techne.