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Large Scale Ex Vivo Expansion of γδ T cells Using Artificial Antigen-presenting Cells

This study demonstrates a GMP-compliant protocol for expanding donor-derived γδ T cells using zoledronic acid, IL-2, and engineered aAPCs. The method achieved significant expansion of Vδ1 and Vδ2 subsets. PBMCs were seeded into G-Rex 100 for the initial enrichment and expansion phases.
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Publications

Homology‑independent targeted insertion (HITI) enables guided CAR knock‑in and efficient clinical scale CAR‑T cell manufacturing

Describes a non-viral CRISPR/Cas9 HITI method for CAR knock-in. Primary T cells were cultivated in G-Rex 24-well/6-well plates or G-Rex 100M vessels.
Publications

Scalable GMP-compliant gene correction of CD4+ T cells with IDLV template functionally validated in vitro and in vivo

Developed a GMP process for gene correction of CD4+ T cells. Medium-scale experiments used G-Rex 6-6M, and large-scale used G-Rex 100CS or 500CS for cell expansion.
Publications

Preclinical Evidence of an Allogeneic Dual CD20xCD22 CAR to Target a Broad Spectrum of Patients with B-cell Malignancies

This study presents preclinical data for allogeneic CD20xCD22 CAR T cells. Cells were cultured in 10 cm G-Rex or 6-well G-Rex devices during the manufacturing process.
Publications

Context-specific synthetic T cell promoters from assembled transcriptional elements

The study creates synthetic promoters for CAR-T cells. T cells nucleofected with piggyBac transposase were transferred to G-REX culture vessels for 21 days of expansion.
Publications

Preparation of cryopreserved chimeric antigen receptor T cells for the locoreogional delivery to the neural axis

Describes a thaw/wash method for CAR-T cells for intracranial delivery. B7-H3-CAR T cells were generated and expanded in G-Rex culture devices using X-VIVO 15 media with cytokines.
Publications

Vγ9Vδ2 T cells expressing a BCMA—Specific chimeric antigen receptor inhibit multiple myeloma xenograft growth

This study generates BCMA-CAR Vg9Vd2 T cells via mRNA electroporation. Vg9Vd2 T cells were expanded using Zometa and co-cultured with aAPCs. Previous work by the group used G-Rex vessels for large-scale expansion.
Publications

Humanized CD19-directed CAR-T Cell Therapy in Pediatric Relapsed/Refractory Acute Lymphoblastic Leukemia With CNSL or Neurological Comorbidity

This clinical study evaluates humanized CD19 CAR-T cells in pediatric R/R ALL with CNS issues. Transduced T cells were cultured, amplified, and harvested over 5–7 days using the G-Rex system.
Publications

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Development of BAFF ligand-based CAR-T cells targeting BAFF-R, BCMA, and TACI. Cells were modified using the TcBuster transposon system and maintained in G-Rex culture vessels.
Publications

Feasibility and preclinical efficacy of CD7-unedited CD7 CAR T cells for T cell malignancies

The study demonstrates manufacturing of CD7 CAR T cells using ibrutinib/dasatinib to prevent fratricide. cGMP manufacturing involved transduction and expansion in G-Rex culture devices for 3 additional days.
Publications

Chimeric antigen receptor structure and manufacturing of clinical grade CAR engineered cells using different bioreactors

This review discusses CAR structure and automated manufacturing platforms. It highlights the G-Rex (M series) and GatheRex pump as a practical, cost-effective, and scalable static culture option for CAR-T expansion.
Publications

Improving cytokine-induced killer cell expansion using a gas-permeable culture method for clinical-scale production

This paper compares CIK cell expansion in G-Rex devices vs standard plates. G-Rex 24-well and 6-well plates were used. G-Rex resulted in significantly higher expansion and IFN-g secretion, supporting clinical-scale production.

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