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Publications

Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade

This study identifies CISH as a negative regulator of T cell effector function. CRISPR/Cas9 deletion of CISH improved TIL neoantigen recognition and efficacy. Clinical-scale manufacturing of CISH KO TILs utilized G-Rex 100 for the rapid expansion protocol (REP).
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Publications

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA

A nonviral CRISPR/Cas9 method using plasmid donor DNA was developed for efficient gene knock-in in primary human T cells. The method was used for TCR and CAR integration. G-Rex 24-well plates were used to culture and expand the edited T cells.
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Publications

CB derived, optimized affinity CD38 CAR-NK cells with CD38 KO show promising in-vivo activity in a Multiple Myeloma model

This poster describes the development of cord blood-derived CD38 CAR-NK cells with CRISPR/Cas9-mediated CD38 knockout. These cells demonstrated potent activity against multiple myeloma. G-Rex 6M plates were utilized to achieve significant cell expansion (approx. 400-fold).
Publications

A Phase I Study to Determine the Maximum Tolerated Dose of ex Vivo Expanded Natural Killer Cells Derived from Unrelated, HLA-Disparate Adult Donors

This Phase I trial evaluated the safety of ex vivo expanded, off-the-shelf NK cells from unrelated donors in patients with malignancies. The NK cells were expanded using a feeder cell platform in G-Rex. The treatment was safe with no GVHD and showed signs of efficacy.
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Publications

Safety and durability of AGT103-T autologous T cell therapy for HIV infection in a Phase 1 trial

A Phase 1 trial evaluated AGT103-T, a genetically modified Gag-specific CD4+ T cell product for HIV. The therapy was safe and increased Gag-specific T cell responses. G-Rex 500M-CS containers were used for the static culture expansion of the cell product during manufacturing.
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Publications

Robust In Vitro Pharmacology of Tmod, a Synthetic Dual-Signal Integrator for Cancer Cell Therapy

This study characterizes the pharmacology of the Tmod system, which uses an activator (CAR/TCR) and a blocker (LIR-1 based) to discriminate tumor from normal cells. Primary T cells transduced with Tmod constructs were cultured and recovered in G-Rex 24-well plates.
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Publications

Cas-CLOVER is a novel high-fidelity nuclease for safe and robust generation of TSCM-enriched allogeneic CAR-T cells

Cas-CLOVER is characterized as a high-fidelity nuclease for generating allogeneic CAR-T cells with high stem cell memory content (TSCM). It shows low off-target activity. The manufacturing process for P-BCMA-ALLO1 utilized G-Rex 100M vessels for cell culture and expansion.
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Publications

Tissue-resident Memory and Circulating T cells are Early Responders to Pre-surgical Cancer Immunotherapy

This study analyzes immune kinetics in oral cancer patients treated with neoadjuvant ICB, finding that tissue-resident memory T cells are key early responders. G-Rex 10 were used to expand T cells for T-Scan antigen discovery screens to identify tumor-associated targets.
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Publications

Fully murine CD105-targeted CAR-T cells provide an immunocompetent model for CAR-T cell biology

This study develops a fully murine CD105 CAR-T cell system to study CAR-T biology in immunocompetent mice. It also validates a human CD105 CAR against melanoma and AML. Human CAR-T cells were expanded in G-Rex 6M well plates following sorting.
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Publications

Cytomegalovirus-Specific T Cells from Third-Party Donors Successfully Treated Refractory Cytomegalovirus Retinitis after Unrelated Umbilical Cord Blood Transplantation

This case study reports the successful treatment of refractory CMV retinitis using third-party donor CMV-specific T cells (CMVST) following cord blood transplantation. G-REX culture were used to culture and expand high purity CD3+ T cells from the donor for the therapy.
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Publications

AIMTM platform: A new immunotherapy approach for viral diseases

This document describes the AIM platform's ability to enrich and expand antigen-specific CD8+ T cells for viral targets. G-Rex culture systems were used for the 14-day expansion process.
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Publications

Natural killer cells in clinical development as non-engineered, engineered, and combination therapies

This review analyzes the landscape of allogeneic NK cell therapies, covering cell sources, engineering, and combination strategies. It highlights the G-Rex platform as a widely used static bioreactor for the clinical manufacturing of NK cell products, including GDA-201 and cord blood-derived NK cells.
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