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Safety and durability of AGT103-T autologous T cell therapy for HIV infection in a Phase 1 trial

A Phase 1 trial evaluated AGT103-T, a genetically modified Gag-specific CD4+ T cell product for HIV. The therapy was safe and increased Gag-specific T cell responses. G-Rex 500M-CS containers were used for the static culture expansion of the cell product during manufacturing.
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Publications

Robust In Vitro Pharmacology of Tmod, a Synthetic Dual-Signal Integrator for Cancer Cell Therapy

This study characterizes the pharmacology of the Tmod system, which uses an activator (CAR/TCR) and a blocker (LIR-1 based) to discriminate tumor from normal cells. Primary T cells transduced with Tmod constructs were cultured and recovered in G-Rex 24-well plates.
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Publications

Cas-CLOVER is a novel high-fidelity nuclease for safe and robust generation of TSCM-enriched allogeneic CAR-T cells

Cas-CLOVER is characterized as a high-fidelity nuclease for generating allogeneic CAR-T cells with high stem cell memory content (TSCM). It shows low off-target activity. The manufacturing process for P-BCMA-ALLO1 utilized G-Rex 100M vessels for cell culture and expansion.
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Publications

Tissue-resident Memory and Circulating T cells are Early Responders to Pre-surgical Cancer Immunotherapy

This study analyzes immune kinetics in oral cancer patients treated with neoadjuvant ICB, finding that tissue-resident memory T cells are key early responders. G-Rex 10 were used to expand T cells for T-Scan antigen discovery screens to identify tumor-associated targets.
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Publications

Fully murine CD105-targeted CAR-T cells provide an immunocompetent model for CAR-T cell biology

This study develops a fully murine CD105 CAR-T cell system to study CAR-T biology in immunocompetent mice. It also validates a human CD105 CAR against melanoma and AML. Human CAR-T cells were expanded in G-Rex 6M well plates following sorting.
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Publications

Cytomegalovirus-Specific T Cells from Third-Party Donors Successfully Treated Refractory Cytomegalovirus Retinitis after Unrelated Umbilical Cord Blood Transplantation

This case study reports the successful treatment of refractory CMV retinitis using third-party donor CMV-specific T cells (CMVST) following cord blood transplantation. G-REX culture were used to culture and expand high purity CD3+ T cells from the donor for the therapy.
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Publications

AIMTM platform: A new immunotherapy approach for viral diseases

This document describes the AIM platform's ability to enrich and expand antigen-specific CD8+ T cells for viral targets. G-Rex culture systems were used for the 14-day expansion process.
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Publications

Natural killer cells in clinical development as non-engineered, engineered, and combination therapies

This review analyzes the landscape of allogeneic NK cell therapies, covering cell sources, engineering, and combination strategies. It highlights the G-Rex platform as a widely used static bioreactor for the clinical manufacturing of NK cell products, including GDA-201 and cord blood-derived NK cells.
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Publications

Local delivery of interleukin 7 with an oncolytic adenovirus activates tumor-infiltrating lymphocytes and causes tumor regression

An oncolytic adenovirus expressing human IL-7 (Ad5/3-E2F-d24-hIL7) was developed to activate TILs within the tumor microenvironment. It increased T cell infiltration and caused tumor regression in animal models. G-Rex culturing plates were used for the rapid expansion of PBMCs in control experiments.
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Publications

Development of a cGMP-compliant process to manufacture donor-derived, CD45RA-depleted memory CD19-CAR T cells

This study establishes a cGMP-compliant manufacturing process for allogeneic CD19-CAR T cells using CD45RA-depleted memory T cells. The process utilizes G-Rex 6M and G-Rex 100M-CS devices for efficient cell expansion, ensuring sufficient yield and phenotype preservation for clinical applications.
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Publications

TEM8 Tri- specific Killer Engager binds both tumor and tumor stroma to specifically engage natural killer cell anti- tumor activity

A Tri-specific Killer Engager (TriKE) targeting TEM8 (cam1615TEM8) was developed to redirect NK cells against both tumor and stromal cells. The molecule enhanced NK cell proliferation and antitumor efficacy in mouse models. Enriched NK cells for in vivo experiments were expanded in G-Rex 6-well plates.
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Publications

Neutralizing antibodies protect mice against Venezuelan equine encephalitis virus aerosol challenge

This study isolates and characterizes neutralizing monoclonal antibodies against Venezuelan equine encephalitis virus (VEEV) from mice and humans. These antibodies target the E2 glycoprotein and protect mice from lethal aerosol challenge. G-Rex devices were used to expand hybridoma clones in serum-free medium.
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