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Publications
CTL Cells
Generation of Tumor-Specific CD8+ T cells
This poster outlines a semi-automated GMP platform using artificial antigen presenting cells (aAPCs) to generate AML-specific CD8+ T cells. Following magnetic enrichment, the antigen-specific T cells were successfully expanded to clinically relevant numbers using G-REX expansion over a 14-day period.
Publications
Car T Cells
Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes
The Cell Trajectory Modulation (CTM) assay provides rapid, label-free profiling of CAR T cell biophysical features like size and deformability. The study compares CAR T cells manufactured in G-Rex gas-permeable plates versus perfusion bioreactors, showing that biophysical signatures correlate with potency and phenotype.
Publications
NK Cells
Enhancement of anti-sarcoma immunity by NK cells engineered with mRNA for expression of a EphA2-targeted CAR
This study demonstrates that NK cells engineered with mRNA to express an EphA2-targeted CAR exhibit enhanced cytotoxicity against pediatric sarcomas. The protocol involved expanding human primary NK cells in G-Rex 24 multi-well cell culture plates using supplemented NK MACS media and activation beads or feeder cells.
Publications
T-Cells
Dual modulation of cytotoxic and checkpoint receptors tunes the efficacy of adoptive Delta One T cell therapy against colorectal cancer
This study investigates the efficacy of Delta One T (DOT) cells against colorectal cancer. It identifies NKG2D ligand upregulation and simultaneous TIGIT/PD1 blockade as strategies to enhance cytotoxicity. The expansion protocol for producing clinical-grade DOT cells utilized the G-REX platform with OpTmizer-CTS medium.
Publications
Car T Cells
Affinity-tuned mesothelin CAR T cells demonstrate enhanced targeting specificity and reduced off-tumor toxicity
This study develops affinity-tuned mesothelin (MSLN) CAR T cells to improve specificity and safety. Low-affinity variants showed effective tumor control with reduced off-tumor toxicity in mice. CAR T cells were expanded in G-Rex 6M well plates during the manufacturing process.
Publications
TCR T Cells
Identification and validation of a T cell receptor targeting KRAS G12V in HLA-A*11:01 pancreatic cancer patients
This study identifies an HLA-A*11:01-restricted TCR targeting KRAS G12V from a pancreatic cancer patient. T cells engineered with this TCR were expanded in G-Rex 24-well plates and showed specific recognition of tumor organoids, enhanced by IFN-gamma priming.
Publications
VST Cells
Donor Variability and PD-1 Expression Limit BK Polyomavirus-specific T-cell Function and Therapy
This study compares conventional and G-Rex systems for expanding BKPyV-specific T cells. G-Rex cultures resulted in higher total cell counts but increased PD-1 expression. The study highlights the need for optimization in adoptive T-cell therapy protocols for BKPyV.
Publications
Car T Cells
Discovery of BMS-986408, a First-In-Class Dual DGKα and DGKζ Inhibitor That Unleashes PD-1 Checkpoint and CAR T-Cell Immunotherapies
This paper reports on BMS-986408, a dual DGK inhibitor that enhances PD-1 and CAR-T therapies. It improves T-cell signaling and effector function. CAR T cells were expanded in G-Rex 6M well plates during the manufacturing process for experimental assays.