Knowledge HQ
Publications
EphA3 CAR T cells are effective against glioblastoma in preclinical models
The study investigates EphA3-targeted CAR T cells for glioblastoma. T cells were transduced and expanded in G-Rex® 24 well plates.
Publications
Building a Better Defense: Expanding and Improving Natural Killer Cells for Adoptive Cell Therapy
A review on NK cell expansion and improvement. It mentions G-Rex® plates as a culture material used in protocols for expanding NK cells, specifically citing studies where G-Rex® 6-well plates or G-Rex® 100M vessels were used.
Publications
synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy
The study develops synNotch-programmed iPSC-NK cells to target CD155 and CD73 in glioblastoma. Cells were expanded for 2-3 weeks in G-Rex® 24 well plates using NK Xpander medium.
Publications
Expansion and Precise CRISPR-Cas9 Gene Repair of Autologous T-Memory Stem Cells from Patients with T-Cell Immunodeficiencies
This protocol details the expansion and gene repair of TSCM cells. It mentions that for scale-up and GMP-compliant experiments, electroporated TSCM can be expanded in G-Rex® 100M gas-permeable culture vessels.
Publications
Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method
The paper describes a feeder-free method to generate AlloCAR-NKT cells from HSPCs. G-Rex® 6M well plates were used for the expansion stage (stage 4) of the NKT cell culture.
Publications
Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes
The study introduces "NeoExpand" to selectively expand neoantigen-reactive TILs. It compares this to rapid expansion protocols (REP). G-Rex® 24-well, 6-well, and 100 were used for the rapid expansion of T cells.
Publications
Virus-Specific T-Cell Therapy for the Management of Viral Infections in the Immunocompromised
A review article discussing VST therapies for immunocompromised patients. It mentions that simplified manufacturing processes use G-Rex® culture devices for the selective activation and expansion of virus-reactive T cells.
Publications
Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay
This paper evaluates a flow cytometric cytotoxicity assay for multiVSTs. It highlights that multiVSTs, expanded in G-Rex® 5M devices for 14 days, show potent cytolytic activity against viral targets that is better detected by this new assay than by chromium release.
Publications
Inosine Induces Stemness Features in CAR-T cells and Enhances Potency
The study shows that inosine induces stemness and metabolic reprogramming in CAR-T cells, improving potency. Clinical-scale manufacturing of GD2-CAR-T cells in inosine-containing media was performed using a semi-closed G-Rex® system.
Publications
Antiviral cellular therapy for enhancing T-cell reconstitution before or after hematopoietic stem cell transplantation (ACES): a two-arm, open label phase II interventional trial of pediatric patients with risk factor assessment
A Phase II trial evaluating third-party VSTs for refractory viral infections in pediatric patients post-HSCT or with IEI. VSTs were expanded using a rapid expansion protocol in G-Rex®-10 for 10 days. Clinical response rate was 62%.
Publications
Human 3D Ovarian Cancer Models Reveal Malignant Cell–Intrinsic and –Extrinsic Factors That Influence CAR T-cell Activity
The study uses 3D ovarian cancer models (spheroids, collagen gels, microfluidics) to study MUC1/TnMUC1 CAR T-cell activity. Resistance mechanisms and fibroblast interactions are explored. Transduced CAR T cells were cultured and expanded in G-Rex® plates for 10 days.
Publications
A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors
This study uses Quality-by-Design (QbD) and Design of Experiments (DOE) to optimize CAR-T expansion. Small-scale DOE studies were conducted in G-Rex® 24-well plates to identify optimal parameters (single activation, short seed train), which were then translated to Ambr 250 bioreactors.
