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Non-viral precision T cell receptor replacement for personalized cell therapy

This phase 1 trial used non-viral CRISPR-Cas9 editing to replace endogenous TCRs with personalized neoantigen-specific TCRs in T cells. Clinical-grade manufacturing of the neoTCR T cell products included activation and expansion steps performed in G-Rex 100M CS
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Bioengineering a pre-vascularized, retrievable, and scalable macroencapsulation device for islet transplantation

This paper presents a novel macroencapsulation device for islet transplantation to treat type 1 diabetes. While the study focuses on device engineering, it references previous work utilizing G-Rex technology for cell culture applications.
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Publications

Efficient ex vivo expansion of conserved element vaccine-specific CD8+ T-cells from SHIV-infected, ART-suppressed nonhuman primates

The authors describe a manufacturing process for expanding virus-specific CD8+ T cells from vaccinated macaques. The final co-culture of T cells, PHA blasts, and feeder cells was rapidly expanded in G-Rex 100 for one week.
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Ablation of SYK kinase from expanded primary human Natural Killer cells via CRISPR/Cas9 enhances cytotoxicity and cytokine production

This study optimized CRISPR/Cas9 editing of expanded primary human NK cells. Ablation of SYK enhanced ADCC and cytokine production. Post-nucleofection, the edited NK cells were transferred to G-Rex 24-well plates for culture and recovery.
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Publications

A chemically inducible IL-2 receptor signaling complex allows for effective in vitro and in vivo selection of engineered CD4+ T cells

The paper describes a system (CISC) that allows engineered T cells and Tregs to be selectively expanded using rapamycin. Clinical-scale manufacturing of the engineered cells was demonstrated using G-Rex 100M CS vessels.
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Publications

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting

The researchers developed a monoclonal antibody against Syrian hamster PD-L1 to enable immunotherapy modeling. In the study, hamster tumor-infiltrating lymphocytes (TILs) were isolated and cultured in six-well G-Rex plates.
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Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation

This study presents Chimeric Engulfment Receptor (CER) T cells that target phosphatidylserine to clear tumors and present antigens. The manufacturing process for these modified T cells involved transferring transduced cells to 6-well G-Rex dishes for expansion.
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Publications

Synapse-tuned CARs enhance immune cell anti-tumor activity

The authors engineered CARs with a PDZ binding motif to improve immune synapse formation, leading to enhanced anti-tumor activity in NK and T cells. During the generation of CD19-CAR T cells, the transduced cells were expanded in G-Rex 10 and then G-Rex 100.
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Publications

Cord blood–derived Vδ2+ and Vδ2− T cells acquire differential cell state compositions upon in vitro expansion

The study characterizes the expansion and differentiation of cord blood-derived gamma-delta T cells. A modified rapid expansion protocol (REP) utilizing G-Rex was employed to achieve scalable expansion of different gamma-delta T cell subtypes.
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Publications

Cell surface marker- based capture of neoantigen- reactive CD8+ T- cell receptors from metastatic tumor digests

The study identifies neoantigen-reactive TCRs from TILs by sorting for CD39, PD-1, and TIGIT co-expression. While these cells are dysfunctional, their TCRs can be used for engineering. Sorted TIL populations were plated in G-Rex 24-well plates for rapid expansion (REP) to assess functional reactivity.
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Publications

How can Cytokine-induced killer cells overcome CAR-T cell limits

This review compares CIK cells to CAR-T cells, highlighting CIK advantages. It notes that G-Rex devices enable efficient, GMP-compliant expansion of CIK cells with simplified protocols, facilitating decentralized manufacturing and reducing costs compared to traditional bioreactors.
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Publications

Optimization of universal allogeneic CAR-T cells combining CRISPR and transposon-based technologies for treatment of acute myeloid leukemia

The authors developed a protocol for generating allogeneic CD33-CAR-T cells using CRISPR-Cas9 to knockout HLA-I/TCR and Sleeping Beauty transposon for CAR delivery. This approach generated functional CAR-T cells. Electroporated CAR-T cells were expanded at large scale using the G-Rex platform.
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