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Transcriptomic analysis reveals optimal cytokine combinations for SARS-CoV-2-specific T cell therapy products

The study identified IL-15 + IL-7 as the optimal cytokine cocktail for expanding SARS-CoV-2-specific T cells (CSTs). Results were translated to a GMP-applicable format using G-Rex 10, which showed improved expansion and specificity, particularly for CD8+ T cells, compared to IL-4 + IL-7.
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Publications

Regression of EGFR positive established solid tumors in mice with the conditionally active T cell engager TAK- 186

This article evaluates TAK-186, a protease-activated T cell engager. Human T cells used for in vivo efficacy studies were expanded using G-Rex 100. TAK-186 demonstrated regression of EGFR+ solid tumors in mice, validating the conditional activation design in a tumor microenvironment.
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Publications

Development of an anti-CAR antibody response in SIV-infected rhesus macaques treated with CD4-MBL CAR/CXCR5 T cells

This study monitored anti-CAR antibody responses in rhesus macaques infused with CD4-MBL CAR/CXCR5 T cells. The therapeutic cells were expanded in G-Rex 6-well plates prior to infusion. All treated animals developed anti-CAR antibodies, which correlated with limited in vivo persistence of the CAR T cells.
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Publications

Hematopoietic Stem Progenitor Cells (HSPC) for Vector Copy Number Determination

This poster presents a G-Rex-based method for expanding lentiviral transduced HSPCs to determine vector copy number (VCN). Compared to standard plates, G-Rex cultures showed higher expansion rates, high viability, and similar lineage phenotypes, providing a less laborious and cost-effective method for QC testing.
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Publications

Impact of cryopreservation on CAR T production and clinical response

This study evaluates the impact of cryopreservation on CAR T cell production. Cells were expanded in G-Rex100, which facilitated simple media top-ups. The study found that cryopreserved PBMCs and CAR T products yielded comparable expansion and clinical responses to fresh materials.
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Publications

Rapid Generation of TCR and CD8ab Transgenic Virus Specific T Cells for Immunotherapy of Leukemia

The authors describe a method to generate TCR and CD8ab transgenic VSTs using IFN-g cytokine capture. G-Rex devices were utilized for the second stimulation expansion phase, yielding high numbers of polyfunctional T cells with simultaneous anti-viral and anti-tumor activity in a shortened timeframe.
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Publications

AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

This paper presents AsCas12a Ultra, an engineered nuclease with high editing efficiency. G-Rex technology was used to expand human CD3+ T cells and activate NK cells during the editing workflows. The nuclease enabled robust multiplex editing and transgene knock-in in various primary cell types.
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Publications

Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive Cancers

The authors engineered high-potency CARs targeting HPV E6 peptide-MHC complexes. Primary T cells transduced with these CARs were expanded in 24-well G-Rex plates supplemented with IL-2. The optimized CARs showed comparable sensitivity and selectivity to clinical TCRs in cytotoxicity assays.
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Publications

Using Allogeneic, Off-the-Shelf, Sars-Cov-2-Specific T Cells to Treat High Risk Patients with COVID-19

This study explores generating banked SARS-CoV-2-specific virus-specific T cells (VSTs) for immunocompromised patients. The manufacturing process utilized G-Rex devices with activating cytokines to produce Th1-polarized, polyfunctional T cells capable of selectively killing viral antigen-expressing targets.
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Publications

CAR-NK Cells Effectively Target SARS-CoV-2-Spike-Expressing Cell Lines In Vitro

The authors developed CAR-NK cells using the S309 antibody to target SARS-CoV-2. Primary S309-CAR-NK cells were expanded in G-Rex plates for 21 days following transduction. These cells demonstrated effective binding and killing of spike-protein expressing target cells in vitro.
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Publications

GMP-Compliant Universal Antigen Presenting Cells (uAPC) Promote the Metabolic Fitness and Antitumor Activity of Armored Cord Blood CAR-NK Cells

This study describes the use of universal antigen presenting cells (uAPC) to expand cord blood CAR-NK cells. G-Rex (M100 series) were used for GMP-grade expansion, enabling a closed system with optimal gas exchange. The process achieved robust expansion and enhanced antitumor activity of the NK cells.
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Publications

Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids

This study reports the spontaneous generation of hyaline cartilage in hiPSC-derived multi-tissue organoids (MTOs) using a xeno- and feeder-free protocol. G-Rex100 were used to culture organoids for up to 30 weeks, overcoming hypoxia limitations. RNA-seq links cartilage formation to BMP signaling.
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