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RNA-seq revealed that ex vivo expansion alters chemokine receptor expression in NK cells, notably upregulating CCR5. G-Rex were used to expand NK cells with feeder cells. CRISPR-mediated CCR5 disruption reduced liver trafficking and increased NK cell presence in circulation in mice.

A high-throughput assay identified optimal cytokine combinations for viral-specific T cell (VST) expansion. The study validated that IL15/IL6 culture conditions in G-Rex10 gas-permeable devices produced VSTs functionally equivalent to standard IL4/IL7 conditions. This method streamlines process development.

The authors generated "Cerberus" T cells (Cb-STs) targeting multiple viruses and Aspergillus fumigatus, with CRISPR/Cas9-mediated glucocorticoid receptor disruption. G-Rex10 devices were used for the culture and expansion of these multi-pathogen specific T cells. Cb-STs maintained function in the presence of dexamethasone.

This study repurposes non-transplantable umbilical cord blood units to generate myeloid dendritic cells (DCs) and bivalent-leukemia-specific T cells. G-Rex were used to expand DCs from CD34+ cells, facilitating scalable production. The generated T cells showed specificity against WT1 and PRAME.

The study presents a manufacturing platform for CD19-specific CAR T cells using enzyme-produced piggyBac transposon DNA and mRNA transposase. G-Rex10 were utilized for large-scale expansion, yielding up to 10^8 CAR-T cells per electroporation. The method allows precise control of vector copy number.

This research optimizes cryopreservation strategies for regulatory T cell (Treg) products. G-Rex were used for the culture and expansion of Treg cells and for transporting fresh nTreg products. Results show that a freezing medium with 5% DMSO significantly improves cell viability and recovery.

This study evaluates differentiating NK cells from iPSCs under chemically defined, serum-free conditions without feeder layers. G-Rex plates were used to expand hiPSC01-derived NK cells and peripheral blood NK cells for 2 weeks, achieving high fold expansion. The method yields functional, cytotoxic NK cells.

This paper investigates the role of p16INK4a in T cell senescence. G-Rex and G-Rex 24-well plates were used to culture T cells for polyclonal activation and antigen-specific line generation, facilitating the study of long-term dysfunction.

This study correlates NK cell receptor repertoires with SARS-CoV-2 clearance. G-Rex 24-well culture plates were used to expand primary NK cells with K562 feeder cells to obtain sufficient cell numbers for functional killing assays.

This study developed mutated GM-CSF-based CAR-T cells targeting CD116/CD131 for AML. The CAR-T cells showed enhanced anti-tumor effects. G-Rex usage was not explicitly mentioned in the provided text.

This study evaluates anti-SIV CAR-T cells in rhesus macaques. Host antibodies inhibited CAR-T expansion in vivo. G-Rex were used during manufacturing to pool and expand CD4+ and CD8+ CAR-T cells prior to infusion into the animals.

This study shows the feasibility of expanding SARS-CoV-2 VSTs from convalescent donors. CD14 negative fractions were seeded in a G-Rex system for a 21-day expansion protocol involving peptide-loaded DCs and PHA blasts, yielding specific T cell products.