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Inhibition of MICA and MICB Shedding Elicits NK cell–mediated Immunity against Tumors Resistant to Cytotoxic T cells

The study demonstrates that blocking MICA/B shedding enables NK cells to target tumors with MHC-I or IFN-gamma pathway mutations. Human NK cells were expanded in vitro using G-Rex 6-well plates. This therapeutic strategy effectively reduced metastases in humanized mouse models.
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Publications

Optimization and Scale-up of Treg Expansion from CD4+ Cells in G-Rex6M plates

This poster details the optimization of Treg expansion using Cloudz™ Treg CD3/CD28 microspheres. The method achieved high purity and cell numbers. Scalability was demonstrated using G-Rex6M plates, which utilize a gas-permeable membrane to support efficient cell growth in serum-free media.
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Publications

Detection of response to tumor microenvironment–targeted cellular immunotherapy using nano-radiomics

This study explores nano-radiomics to assess response to MDSC-directed NK cell therapy in solid tumors. Human NK cells were activated and expanded in G-Rex devices for in vivo testing. Radiomic analysis of CT images successfully identified treatment responses that conventional tumor metrics failed to detect.
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Publications

Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting Cells

This study develops renewable artificial antigen presenting cells (aAPCs) for T cell expansion and transduction. Using G-Rex , aAPCs supported efficient production of CAR T cells and TILs with less exhaustion and higher potency than bead-based methods, offering a cost-effective manufacturing alternative.
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Publications

Rapidly expanded partially HLA DRB1–matched fungus-specific T cells mediate in vitro and in vivo antifungal activity

The authors describe a GMP-compliant method to rapidly expand fungus-specific T cells using CD137 selection and short-term culture. G-Rex cell culture plates were used for the in vitro expansion of the enriched CD137+ population. The resulting T cells demonstrated potent antifungal activity against Aspergillus.
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Publications

Head- to- head comparison of in- house produced CD19 CAR- T cell in ALL and NHL patients

This study compares in-house produced CD19 CAR-T cells for ALL and NHL. Cells from ALL patients expanded better. Production utilized G-Rex100 , enabling rapid generation of therapeutic doses. The study highlights phenotypic differences based on the patient's disease.
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Publications

Production of Lentiviral Vectors Using Suspension Cells Grown in Serum-free Media

The authors developed a scalable, serum-free process for lentiviral vector production using suspension-adapted SJ293TS cells. The vectors efficiently transduced human T cells and CD34+ cells. G-Rex plates were employed to expand the transduced T cells for assessing chimeric antigen receptor expression and titer.
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Publications

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

This study compares monocyte-derived dendritic cell (Mo-DC) production in fluorinated ethylene propylene bags versus polystyrene plates. Mo-DCs generated in bags showed comparable phenotype and ability to activate T cells. G-Rex were utilized for the T cell co-culture steps to assess activation potency.
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Publications

Potential clinical application of tumor-infiltrating lymphocyte therapy for ovarian epithelial cancer prior or post-resistance to chemotherapy

Evaluated TIL ACT for ovarian cancer. TILs were expanded using a 'TIL 3.0' method combining IL-2 with agonistic 4-1BB and anti-CD3 antibodies in G-Rex 10. This method significantly increased CD8+ TIL yield and success rates compared to IL-2 alone, producing tumor-reactive T cells in 2-3 weeks.
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Publications

Identification of protective T-cell antigens for smallpox vaccines

Study identifying E3L as a key antigen for protective T-cell immunity against vaccinia virus. Virus-specific T cells (VSTs) were generated, referencing previous protocols using G-Rex cultureware.
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Publications

Electroporation of NKG2D RNA CAR Improves Vg9Vd2 T Cell Responses against Human Solid Tumor Xenografts

Study improving Vg9Vd2 T cell responses using NKG2D RNA CARs. Cells were expanded using K562 aAPCs in G-Rex vessels before electroporation. CAR-modified cells showed enhanced cytotoxicity against solid tumors in vitro and in vivo.
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Publications

“Mini” bank of only 8 donors supplies CMV-directed T cells to diverse recipients

Production of a third-party CMV-specific T cell (CMVST) bank. Cells were expanded using G-Rex 5 and G-Rex 100M. The bank successfully provided matched lines for 96.6% of screened patients with 100% response rate in treated patients.
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