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Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood

Developed a rapid method to produce multivirus-specific T cells from cord blood using overlapping PepMixes. G-Rex culture devices maintained or improved antigen recognition and overcame limitations in expanding sufficient cells for clinical application, allowing large-scale production.
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Publications

Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells

A single-cell methodology integrating cellular phenotyping and dynamic cytokine secretion was developed using nanowell arrays. G-Rex 100M were used for the rapid expansion protocol (REP) of tumor-infiltrating lymphocytes (TILs) to achieve desired cell numbers for experiments.
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Publications

Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells

Describes a method for large-scale isolation of extracellular vesicles (EVs) from activated NK cells. The G-Rex cell culture device allowed for the expansion of 1-3 billion aNK cells per 100 cm2, increasing EV production while reducing cost and complexity. Isolated EVs showed cytotoxicity against cancer cells.
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Publications

A single exercise bout enhances the manufacture of viral-specific T-cells from healthy donors: implications for allogeneic adoptive transfer immunotherapy

This study showed that collecting PBMCs after exercise increases the yield of VSTs. The VSTs were expanded in G-Rex10 for 8 days, demonstrating that exercise is a simple adjuvant for improving VST manufacture.
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Publications

Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34+ cells of juvenile myelomonocytic leukemia

The authors developed GM-CSF Receptor (CD116) CAR T cells using the piggyBac transposon system. The transgenic CAR T cells were expanded in G-Rex 10 devices to generate effector cells for testing against JMML cells.
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Publications

Safety of Intra-Arterial Injection With Tumor-Activated T Cells to the Rabbit Brain Evaluated by MRI and SPECT/CT

This study evaluated the safety of intra-arterial TIL delivery in rabbits. The human TILs used for infusion were expanded from glioblastoma tumors using G-Rex to obtain sufficient cell numbers for the experiment.
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Publications

Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy

Describes a GMP-compliant method for expanding NK cells using K562 feeder cells in G-Rex 10 and G-Rex 100. The method yields large numbers of functional NK cells in 10 days without feeding, and supports efficient retroviral transduction.
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Publications

Plasticity and Aggregation of Juvenile Porcine Islets in Modified Culture: Preliminary Observations

The study reports that culturing juvenile porcine islets in Wilson Wolf gas-permeable (G-Rex) allows them to aggregate into functional 'pancreatites'. These flasks were also used for shipping the islets, maintaining viability and function.
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Publications

Manufacture of GMP-compliant functional adenovirus-specific T-cell therapy for treatment of post-transplant infectious complications

This paper details the GMP manufacture of Cytovir ADV. The process utilizes a closed G-Rex 10 cell culture device for a 10-day expansion of adenovirus-specific T cells, requiring minimal intervention and achieving high purity and potency.
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Publications

Expansion of tumor-infiltrating lymphocytes (TIL) from human pancreatic tumors

The study demonstrates successful expansion of tumor-reactive TILs from pancreatic cancer. The Rapid Expansion Protocol (REP) was performed using GREX10 to stimulate and expand the TILs with anti-CD3 and feeder cells.
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Publications

The beneficial effects of a gas-permeable for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity

This paper investigates the use of G-Rex for TIL rapid expansion. Results show G-Rex rescues poor TIL growth, preserves clonal diversity, and yields cells with a more oxidative metabolic phenotype (higher mitochondrial capacity) compared to traditional flasks/bags.
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Publications

Efficient mRNA-Based Genetic Engineering of Human NK Cells with High-Affinity CD16 and CCR7 Augments Rituximab-Induced ADCC against Lymphoma and Targets NK Cell Migration toward the Lymph Node-Associated Chemokine CCL19

This study shows efficient mRNA electroporation of NK cells. Clinical-grade NK cells were expanded ex vivo using G-Rex prior to electroporation, allowing for the generation of large numbers of highly cytotoxic cells.
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