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This review discusses establishing academic GMP facilities for cell therapy. It highlights the use of closed systems like G-Rex® (referenced via Harari et al.) to improve efficiency and compliance. The article emphasizes the benefits of hospital-based manufacturing for personalized therapies like TILs.

Abstract describing the preclinical validation of IL-8 receptor-modified CD70 CAR T cells. The G-Rex® system was used for ex vivo expansion, achieving approximately 1200-fold expansion in 2 weeks. The optimized process allows for clinical scale production using small blood volumes.

The authors developed the QuIK assay to measure proliferation and killing kinetics of engineered T cells. Primary human T cells were transduced and expanded in 24-well G-Rex® plates. The assay provides detailed insights into the functional biology of CAR-T and TCR-T cells over time.

This research demonstrates that photodepletion with 2-Se-Cl selectively removes alloreactive T cells to prevent GVHD without compromising antitumor immunity. G-Rex® devices were utilized for the initial co-culture and priming of splenocytes before the photodepletion step.

This study characterizes CMV-specific T lymphocytes (VSTs) expanded rapidly for adoptive immunotherapy. Using G-Rex® technology with IL-2, IL-7, and IL-15, researchers achieved a scalable process yielding functional, cytotoxic T cells. The method ensures high specificity and safety for treating viral infections.

The authors developed a GMP-compliant TIL expansion protocol using G-Rex® and the Xuri bioreactor. Pre-REP expansion in G-Rex® resulted in higher cell numbers compared to 24-well plates. The closed-system process reduced operator time and produced functional TILs suitable for clinical trials.

The study demonstrates that blocking MICA/B shedding enables NK cells to target tumors with MHC-I or IFN-gamma pathway mutations. Human NK cells were expanded in vitro using G-Rex® 6-well plates. This therapeutic strategy effectively reduced metastases in humanized mouse models.

This poster details the optimization of Treg expansion using Cloudz™ Treg CD3/CD28 microspheres. The method achieved high purity and cell numbers. Scalability was demonstrated using G-Rex®6M plates, which utilize a gas-permeable membrane to support efficient cell growth in serum-free media.

This study explores nano-radiomics to assess response to MDSC-directed NK cell therapy in solid tumors. Human NK cells were activated and expanded in G-Rex® devices for in vivo testing. Radiomic analysis of CT images successfully identified treatment responses that conventional tumor metrics failed to detect.

This study develops renewable artificial antigen presenting cells (aAPCs) for T cell expansion and transduction. Using G-Rex® , aAPCs supported efficient production of CAR T cells and TILs with less exhaustion and higher potency than bead-based methods, offering a cost-effective manufacturing alternative.

The authors describe a GMP-compliant method to rapidly expand fungus-specific T cells using CD137 selection and short-term culture. G-Rex® cell culture plates were used for the in vitro expansion of the enriched CD137+ population. The resulting T cells demonstrated potent antifungal activity against Aspergillus.

This study compares in-house produced CD19 CAR-T cells for ALL and NHL. Cells from ALL patients expanded better. Production utilized G-Rex®100 , enabling rapid generation of therapeutic doses. The study highlights phenotypic differences based on the patient's disease.