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Describes a static culture protocol for expanding NK cells using K562-mb15-41BBL feeder cells in G-Rex devices. The G-Rex system yielded higher fold expansion (>100-fold) and total cell numbers compared to gas-permeable bags, with no feeding required during the 8-10 day culture period

Details a simplified TIL production method using G-Rex. G-Rex10 is used for initial culture from tumor fragments/digests, and G-Rex100 for rapid expansion (REP). This method reduces media use by 3-4 times and requires fewer vessels and labor compared to traditional 24-well plates and bags

Describes a rapid method (10 days) to generate multivirus-specific CTLs using pepmixes and IL4/IL7. The method uses the G-Rex10 device for stimulation and expansion, allowing the production of large numbers (1x10^8) of virus-specific T cells from a small starting number of PBMCs with minimal manipulation

Compares the WAVE to static gas-permeable bags for TIL and PBL expansion. While total cell numbers were comparable, the WAVE produced a higher percentage of CD4+ cells and a less activated phenotype. The study references G-Rex (Vera 2010) as an alternative static option

Describes generating CTL lines targeting multiple tumor-associated antigens (SSX2, MAGEA4, Survivin, etc.) for lymphoma. Uses optimized cytokine cocktails (IL-7, IL-12, IL-15, IL-6/IL-27) for activation. While not explicitly detailed in the excerpts, G-Rex is standard for this group's expansion protocols

Describes using the PiggyBac transposon system to modify EBV-CTLs to express HER2-CARs. The G-Rex device (G-Rex10 and G-Rex100) was used to expand the gene-modified CTLs, supporting long-term survival and stable transgene expression. G-Rex allowed efficient expansion of clinical-grade cells with minimal manipulation

Reviews optimization of antigen-specific T cell manufacturing. Highlights the G-Rex device as a revolutionary tool that increases cell yields by 3-20 fold while reducing manipulation. G-Rex allows unrestricted media depth due to gas exchange from the bottom, linearly scalable from research to GMP production

Outlines GMP manufacturing of LMP1/LMP2-specific CTLs for EBV-associated lymphoma. Describes using G-Rex devices for the second stimulation and expansion phases to support high-density cell growth. G-Rex simplifies the process and reduces labor compared to traditional 24-well plates while maintaining cell quality

Evaluates G-Rex for expanding antigen-specific CTLs. G-Rex supports 3-20 fold higher cell output than 24-well plates by improving gas exchange and allowing deeper media. The device simplifies manufacturing by reducing interventions and supports high-density culture of CTLs and CAR-T cells without compromising function

Investigates culturing pancreatic islets on gas-permeable silicone rubber membranes. This method allows for higher surface densities (up to 4000 IE/cm²) and media depths compared to standard without hypoxic damage. G-Rex technology principles are tested here for islet survival and function

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