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The study explores antibody responses from tumor-infiltrating B cells against viral antigens in cancer patients. G-Rex® were used to expand tumor-infiltrating lymphocytes (TILs) from tumor tissue, facilitating the analysis of immune reactivity in the tumor microenvironment.

This study demonstrates a method for generating tumor-specific TCR-T cells using CRISPR/Cas9 for endogenous TCR knockout and AAV for TCR insertion. G-Rex® 24-well plates were utilized for T cell expansion, supporting cell viability and growth during the editing process.

This Phase I trial assessed the safety of infusing autologous expanded Tregs into kidney transplant recipients to induce tolerance. G-Rex® were used to expand Tregs, achieving robust cell numbers and meeting all clinical release criteria without adverse events.

A method to generate virus-reactive T cells from cord blood using accelerated co-cultured dendritic cells (acDC). The use of G-Rex® 10 significantly increased the yield of antigen-reactive CD8+ T cells compared to standard plates, enabling therapeutic dosing.

Evaluated a closed-system manufacturing process for TCR-transduced T-cells. The rapid expansion phase utilized closed G-Rex® 500CS devices, yielding ~20 x 10^9 highly functional T-cells from a single vessel, improving safety and consistency.

Reviews improvements to TIL manufacturing. Implementing G-Rex® 100MCS devices for the Rapid Expansion Protocol (REP) reduced technical interventions, media usage, and contamination risk while maintaining comparable tumor specificity to flask/bag methods.

A scalable protocol for producing red blood cells from adult PBMCs was developed. The G-Rex® allowed for massive expansion (3x10^6 fold) of erythroblasts and supported efficient differentiation and enucleation, comparable to standard culture dishes.

Developed "universal" CAR T cells using a lentiviral vector coupling CAR expression with CRISPR/Cas9 gene editing. G-Rex® were used for conventional control cultures to compare against the automated CliniMACS Prodigy system.

Prospective analysis of TIL therapy in metastatic melanoma. The Rapid Expansion Protocol (REP) was performed using G-Rex® 100M, contributing to the generation of infusion products. High TIL numbers were associated with response.

Review comparing cell culture systems (bags vs flasks) for dendritic cell production. Discusses gas permeability and materials. References Wilson Wolf patents related to gas-permeable devices as examples of improved culture methods utilizing silicone rubber membranes.

Investigated methods to overcome fratricide in NKG2D-CAR T cell production. The manufacturing process included an expansion phase in G-Rex® containers, which was part of the upscaling strategy to produce sufficient cell yields.

Generated MUC1-specific CAR T cells co-expressing an inverted cytokine receptor (4/7ICR) for breast cancer. Co-culture experiments to monitor anti-tumor activity were conducted in 6-well G-Rex® devices using a 3D algimatrix bioscaffold.