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Describes a GMP-compliant method for expanding NK cells using K562 feeder cells in G-Rex® 10 and G-Rex® 100. The method yields large numbers of functional NK cells in 10 days without feeding, and supports efficient retroviral transduction.

The study reports that culturing juvenile porcine islets in Wilson Wolf gas-permeable (G-Rex®) allows them to aggregate into functional 'pancreatites'. These flasks were also used for shipping the islets, maintaining viability and function.

This paper details the GMP manufacture of Cytovir ADV. The process utilizes a closed G-Rex® 10 cell culture device for a 10-day expansion of adenovirus-specific T cells, requiring minimal intervention and achieving high purity and potency.

The study demonstrates successful expansion of tumor-reactive TILs from pancreatic cancer. The Rapid Expansion Protocol (REP) was performed using GREX10 to stimulate and expand the TILs with anti-CD3 and feeder cells.

This paper investigates the use of G-Rex® for TIL rapid expansion. Results show G-Rex® rescues poor TIL growth, preserves clonal diversity, and yields cells with a more oxidative metabolic phenotype (higher mitochondrial capacity) compared to traditional flasks/bags.

This study shows efficient mRNA electroporation of NK cells. Clinical-grade NK cells were expanded ex vivo using G-Rex® prior to electroporation, allowing for the generation of large numbers of highly cytotoxic cells.

The authors developed a method for early transduction of trivirus-specific T cells. The clinical SOP utilized G-Rex®100 for the second stimulation phase to rapidly expand transduced VSTs to clinically relevant numbers.

The study validates a xeno-free serum replacement for T cell expansion. It demonstrates that combining OpTmizer medium with CTS Immune Cell SR in G-Rex® culture vessels promotes optimal expansion of antigen-specific T cells compared to standard flasks.

This document provides instructions for Lonza's Fresh Human Pancreatic Islets. It highlights that the islets are shipped in Wilson Wolf G-Rex® gas permeable cell culture devices (G-Rex®10 or G-Rex®100) to maintain viability during transport and culture.

The study found that IL-12 conditioning increases IL-7Rα, crucial for T cell persistence. For clinical translation, TCR-transduced human T cells were expanded using a rapid expansion protocol in G-Rex® 100 to generate large numbers of cells for analysis.

This paper establishes a clinical-compliant protocol to generate MiHA-specific T-cell lines. The priming phase utilized G-Rex®10 to maximize cell expansion and DC-T cell interactions starting from limited PBMCs, followed by a rapid expansion protocol.

The study demonstrates that AML exosomes suppress hematopoiesis by altering stromal cells and progenitors. To model physiological hypoxia, AML cells were cultured in G-Rex® in a low-oxygen chamber, which increased exosome release compared to normoxia.