Knowledge HQ

Literature

G-Rex Grant Program Outline

Review the official description and outline of the new G-Rex® Grant Program, which empowers CGT manufacturers with resources and expertise. This $20 million initiative by ScaleReady, Wilson Wolf Manufacturing, Bio-Techne, and CellReady is designed to propel the advancement of cell and gene therapies (CGT).

Videos

Multiply Labs and Wilson Wolf Collaborate to Automate G-Rex for Cell Therapy Manufacturing

Multiply Labs, a robotics company developing industry-leading automated manufacturing systems to produce individualized drugs, and Wilson Wolf, an innovative leader in cell and gene-modified cell (CGT) manufacturing technology, today announced a collaboration around the automation of Wilson Wolf’s G-Rex® bioreactors.

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Scientific Posters

AgonOx Cell Therapy Manufacturing Process Scientific Poster

To better optimize their manufacturing process for a current clinical trial and IND amendment, AgonOx and Providence began to look for products to close and streamline key portions of the established manufacturing process for a shared project. It was imperative to find products that would maintain established levels of cell recovery, viability, and efficacy.

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Application Notes

Analytical Tools to Evaluate CAR-T Cell Signaling & Activation App Note

CAR-T cell therapy has emerged as a powerful weapon in the fight against specific B-cell malignancies. Its success has fueled significant research efforts to broaden its reach to encompass a wider range of hematologic and solid tumors. Optimizing CAR-T cell therapy hinges on a deeper understanding of the intricate signaling pathways that govern these potent immune cells. This includes their ability to infiltrate tumors, evade antigen escape mechanisms, resist immunosuppression, and minimize potentially fatal toxicities. To achieve this, researchers require a comprehensive characterization of the signaling molecules involved, encompassing the extracellular, membrane-bound, and intracellular compartments. However, this endeavor is often hampered by limited and complex sample types. The Wilson Wolf G-Rex platform offers a unique solution for large-scale expansion of CAR-T cells, enabling researchers to study these vital communication networks within larger, more robust cell populations. This facilitates a deeper understanding of CAR-T cell function and paves the way for the development of safer and more effective CAR-T therapies. Unleash the Full Potential of CAR-T Cells: Learn More Understanding these vital communication networks within CAR-T cells is critical to unlocking their full potential. Download our free App Note: Analytical Tools to Evaluate CAR-T Cell Signaling & Activation to explore the latest advancements in research methods for optimizing CAR-T cell therapy.

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Case Studies

G-Rex: Expanding the Capabilities of Scalability

When global events affected the availability of products used in the manufacture of cell therapies, teams needed to explore alternative manufacturing methods. This supply chain crisis emphasized the need to establish redundant processes to address production obstacles for critical stages of cell therapy manufacturing.

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Scientific Posters

TcBuster transposase for robust delivery of multicistronic therapeutic cargo - Scientific Poster

TcBuster-MTM (TcB-MTM) is a commercially available non-viral transposase-based editing platform that overcomes current viral limitations. TcBuster is found in the red flour beetle and is a member of the hAT family of transposases. Using directed evolution, we engineered a hyperactive mutant (TcB-M) that has improved transposition rates using less mRNA transposase and NanoplasmidTM DNA transposon.

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Scientific Posters

Feeder-Free Human iNK Differentiation Process - Scientific Poster

Current iNK differentiation protocols typically utilize xenogenic feeder cells, which could be problematic for patient safety, or they lack robustness and are highly variable in differentiation efficiency. Using animal-free small molecules and recombinant proteins, Bio-Techne developed a feeder-free differentiation process to reliably generate iNKs at scale by optimizing for CD34+ hematopoietic stem cells (iHSC) prior to downstream NK differentiation.

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Protocols

ExCellerate NK Media Feeder-Cell G-Rex Culture Process

Download the ExCellerate NK Media Feeder-Cell G-Rex Culture Process

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Protocols

ExCellerate NK Media Feeder-Free G-Rex Culture Protocol

Download the protocol to support a feeder-free process in G-Rex cell culture.

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Literature

ExCellerate NK Media Flyer

ExCellerate NK Media offers a robust and versatile solution for NK cell cultures, designed to transition from research to clinical application seamlessly.

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Literature

Liquid Cytokines Brochure

Streamline your cell culture process with our new liquid frozen formulations of animal-free RUO and GMP IL-2, IL-7, and IL-15. These ready-to-use cytokines eliminate reconstitution errors while still providing the same potency and consistency as our lyophilized proteins. Simply thaw and add to your cell culture.

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Application Notes

CAR NK-92 cell–mediated depletion of residual TCR+ cells

Utilization of G-Rex by introducing a new scalable depletion method: Coculturing target CAR T cells with an NK-92 cell line in G-Rex to purify their CAR T cells and promote CAR T cell expansion at the same time.

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