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Manufacture of GMP-compliant functional adenovirus-specific T-cell therapy for treatment of post-transplant infectious complications

This paper details the GMP manufacture of Cytovir ADV. The process utilizes a closed G-Rex 10 cell culture device for a 10-day expansion of adenovirus-specific T cells, requiring minimal intervention and achieving high purity and potency.
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Publications

Expansion of tumor-infiltrating lymphocytes (TIL) from human pancreatic tumors

The study demonstrates successful expansion of tumor-reactive TILs from pancreatic cancer. The Rapid Expansion Protocol (REP) was performed using GREX10 to stimulate and expand the TILs with anti-CD3 and feeder cells.
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Publications

The beneficial effects of a gas-permeable for expansion of Tumor-Infiltrating lymphocytes as reflected in their mitochondrial function and respiration capacity

This paper investigates the use of G-Rex for TIL rapid expansion. Results show G-Rex rescues poor TIL growth, preserves clonal diversity, and yields cells with a more oxidative metabolic phenotype (higher mitochondrial capacity) compared to traditional flasks/bags.
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Publications

Efficient mRNA-Based Genetic Engineering of Human NK Cells with High-Affinity CD16 and CCR7 Augments Rituximab-Induced ADCC against Lymphoma and Targets NK Cell Migration toward the Lymph Node-Associated Chemokine CCL19

This study shows efficient mRNA electroporation of NK cells. Clinical-grade NK cells were expanded ex vivo using G-Rex prior to electroporation, allowing for the generation of large numbers of highly cytotoxic cells.
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Early transduction produces highly functional chimeric antigen receptor-modified virus-specific T-cells with central memory markers: a Production Assistant for Cell Therapy (PACT) translational application

The authors developed a method for early transduction of trivirus-specific T cells. The clinical SOP utilized G-Rex100 for the second stimulation phase to rapidly expand transduced VSTs to clinically relevant numbers.
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Ex vivo expansion of human T cells for adoptive immunotherapy using the novel Xeno-free CTS Immune Cell Serum Replacement

The study validates a xeno-free serum replacement for T cell expansion. It demonstrates that combining OpTmizer medium with CTS Immune Cell SR in G-Rex culture vessels promotes optimal expansion of antigen-specific T cells compared to standard flasks.
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Publications

Cells on Demand™ Fresh Human Pancreatic Islets Technical Information & Instructions

This document provides instructions for Lonza's Fresh Human Pancreatic Islets. It highlights that the islets are shipped in Wilson Wolf G-Rex® gas permeable cell culture devices (G-Rex10 or G-Rex100) to maintain viability during transport and culture.
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Publications

Effector CD8+ T cell engraftment and anti-tumor immunity in lymphodepleted hosts is IL-7Rα dependent

The study found that IL-12 conditioning increases IL-7Rα, crucial for T cell persistence. For clinical translation, TCR-transduced human T cells were expanded using a rapid expansion protocol in G-Rex 100 to generate large numbers of cells for analysis.
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Publications

Defining novel parameters for the optimal priming and expansion of minor histocompatibility antigen-specific T cells in culture

This paper establishes a clinical-compliant protocol to generate MiHA-specific T-cell lines. The priming phase utilized G-Rex10 to maximize cell expansion and DC-T cell interactions starting from limited PBMCs, followed by a rapid expansion protocol.
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Publications

Coordinate regulation of residual bone marrow function by paracrine trafficking of AML exosomes

The study demonstrates that AML exosomes suppress hematopoiesis by altering stromal cells and progenitors. To model physiological hypoxia, AML cells were cultured in G-Rex in a low-oxygen chamber, which increased exosome release compared to normoxia.
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Publications

Therapeutic bispecific T-cell engager antibody targeting the intracellular oncoprotein WT1

This study describes a BiTE derived from a TCR-mimic antibody against WT1. It induced T-cell cytotoxicity against WT1+ tumors and epitope spreading. Transduced EBV-specific T lymphocytes used in in vivo tracking studies were cultured in G-Rex.
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Publications

Go-CART: an animal-free system for the assessment of CAR T cell function

The authors developed Go-CART, a six-chambered device to assess CAR T cell function without animals. The device features a gas-permeable base, allowing prolonged T cell-tumor interaction (>2 weeks) without medium replenishment, similar to G-Rex technology (co-author John Wilson is from Wilson Wolf).
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