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Publications

Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells

A scalable protocol for producing red blood cells from adult PBMCs was developed. The G-Rex allowed for massive expansion (3x10^6 fold) of erythroblasts and supported efficient differentiation and enucleation, comparable to standard culture dishes.
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Publications

Long Terminal Repeat CRISPR-CAR-Coupled “Universal” T Cells Mediate Potent Anti-leukemic Effects

Developed "universal" CAR T cells using a lentiviral vector coupling CAR expression with CRISPR/Cas9 gene editing. G-Rex were used for conventional control cultures to compare against the automated CliniMACS Prodigy system.
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Publications

Prospective analysis of adoptive TIL therapy in patients with metastatic melanoma: response, impact of anti-CTLA4, and biomarkers to predict clinical outcome

Prospective analysis of TIL therapy in metastatic melanoma. The Rapid Expansion Protocol (REP) was performed using G-Rex 100M, contributing to the generation of infusion products. High TIL numbers were associated with response.
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Publications

Bags versus: a comparison of cell culture systems for the production of dendritic cell–based immunotherapies

Review comparing cell culture systems (bags vs flasks) for dendritic cell production. Discusses gas permeability and materials. References Wilson Wolf patents related to gas-permeable devices as examples of improved culture methods utilizing silicone rubber membranes.
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Publications

Overcoming Target Driven Fratricide for T Cell Therapy

Investigated methods to overcome fratricide in NKG2D-CAR T cell production. The manufacturing process included an expansion phase in G-Rex containers, which was part of the upscaling strategy to produce sufficient cell yields.
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Publications

CAR T cell therapy for breast cancer: harnessing the tumor milieu to drive T cell activation

Generated MUC1-specific CAR T cells co-expressing an inverted cytokine receptor (4/7ICR) for breast cancer. Co-culture experiments to monitor anti-tumor activity were conducted in 6-well G-Rex devices using a 3D algimatrix bioscaffold.
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Publications

Lym-1 Chimeric Antigen Receptor T Cells Exhibit Potent Anti-Tumor Effects against B-Cell Lymphoma

Evaluated Lym-1 CAR T cells against B-cell lymphoma. Primary T cells were transduced and expanded in 24-well G-Rex plates. The cells showed potent anti-tumor effects in vitro and in vivo against Raji xenografts.
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Publications

4-1BB agonist focuses CD8+ tumor-infiltrating T-cell growth into a distinct repertoire capable of tumor recognition in pancreatic cancer

Study on 4-1BB agonist enhancing CD8+ TIL growth from pancreatic ductal adenocarcinoma (PDAC). The Rapid Expansion Protocol (REP) was performed in G-Rex 10 devices, enabling the exponential growth of sorted CD8+ and bulk TIL populations for functional assays.
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Publications

A Cryopreserved Tumor Infiltrating Lymphocyte (TIL) Product for LN-144, Generated with an Abbreviated Method Suitable for High Throughput Commercial Manufacturing Exhibits Favorable Quality Attributes For Adoptive Cell Transfer

Describes Iovance's Gen 2 manufacturing process for LN-144 (TILs), an abbreviated 22-day closed process yielding cryopreserved product. The Gen 2 process uses a single closed bioreactor to improve scalability and throughput compared to the Gen 1 method.
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Publications

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens

Describes a culture method using GM-CSF, TLR agonists, and IL-7 to expand antigen-specific T-cells from PBMC. The study confirmed the culture method could be scaled up using G-Rex 100M vessels, showing effectiveness comparable to 24-well plates for patient treatment.
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Publications

Mesothelin as a novel biomarker and immunotherapeutic target in human glioblastoma

Investigates mesothelin as an immunotherapeutic target in glioblastoma. Mesothelin-specific T-cell responses were detected in TILs. G-Rex were utilized for the further expansion of TILs isolated from glioma tumor tissue to achieve necessary cell numbers.
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Publications

Clinical-Scale Rapid Autologous BK Virus-Specific T Cell Line Generation From Kidney Transplant Recipients With Active Viremia for Adoptive Immunotherapy

Describes a clinical-scale protocol to generate autologous BK virus-specific T cell lines from kidney transplant recipients. G-Rex10 vessels were used for all T cell cultures, allowing for rapid expansion and generation of antigen-specific T cell lines suitable for adoptive immunotherapy.
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