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Publications

Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma

This study identified neoepitopes recognized by TILs from glioblastoma patients. G-Rex were used to expand TILs from tumor digests in the presence of OKT3 and irradiated feeder cells, providing sufficient cells for immune reactivity testing.
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Publications

Utilizing T-cell activation signals 1, 2 and 3 for tumor-infiltrating lymphocytes (TIL) expansion: the advantage over the sole use of interleukin-2 in cutaneous and uveal melanoma

A novel TIL expansion method combining IL-2, anti-4-1BB, and OKT3 was developed. Using small G-Rex 10 devices allowed for successful expansion from fewer tumor fragments and reduced culture time compared to standard methods, especially for uveal melanoma.
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Publications

TCRαβ/CD3 disruption enables CD3-specific antileukemic T cell immunotherapy

This paper reports the creation of anti-CD3 CAR-T cells (3CAR) using TALEN-mediated TCR disruption to avoid self-killing. G-Rex were used to scale up the production, allowing 3CAR T cells to expand 100-fold and self-enrich for the TCR-negative population.
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Publications

Efficient Enrichment of Gene-Modified Primary T Cells via CCR5-Targeted Integration of Mutant Dihydrofolate Reductase

The study demonstrates a method to enrich gene-edited CD4+ T cells using methotrexate selection. G-Rex 10 were employed for the long-term expansion of lentivirus-modified T cells, facilitating the generation of large numbers of selected cells.
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Publications

Manufacturing development and clinical production of NKG2D Chimeric Antigen Receptor-expressing T cells for autologous adoptive cell therapy

A 9-day manufacturing process for NKG2D CAR-T cells was developed for a Phase I trial. G-Rex 100 were utilized for the ex vivo expansion step, enabling the production of therapeutic doses of viable and functional CAR-T cells from patient material.
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Publications

Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells

This paper describes an improved method for generating CAR-T cells using the piggyBac transposon system. G-Rex10 culture were used for the expansion phase, contributing to the generation of clinically relevant numbers of CAR-T cells with a memory phenotype.
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Publications

CMV and EBV targets recognized by tumor-infiltrating B lymphocytes in pancreatic cancer and brain tumors

The study explores antibody responses from tumor-infiltrating B cells against viral antigens in cancer patients. G-Rex were used to expand tumor-infiltrating lymphocytes (TILs) from tumor tissue, facilitating the analysis of immune reactivity in the tumor microenvironment.
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Publications

Generation of Tumor-Specific (tg-TCR) T Cells Using CRISPR-Mediated In-Locus Insertion Combined with Endogenous TCR Knockout

This study demonstrates a method for generating tumor-specific TCR-T cells using CRISPR/Cas9 for endogenous TCR knockout and AAV for TCR insertion. G-Rex 24-well plates were utilized for T cell expansion, supporting cell viability and growth during the editing process.
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Publications

A Phase I Clinical Trial with Ex Vivo Expanded Recipient Regulatory T cells in Living Donor Kidney Transplants

This Phase I trial assessed the safety of infusing autologous expanded Tregs into kidney transplant recipients to induce tolerance. G-Rex were used to expand Tregs, achieving robust cell numbers and meeting all clinical release criteria without adverse events.
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Publications

In Vitro Expansion of Anti-viral T Cells from Cord Blood by Accelerated Co-cultured Dendritic Cells

A method to generate virus-reactive T cells from cord blood using accelerated co-cultured dendritic cells (acDC). The use of G-Rex 10 significantly increased the yield of antigen-reactive CD8+ T cells compared to standard plates, enabling therapeutic dosing.
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Publications

Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules

Evaluated a closed-system manufacturing process for TCR-transduced T-cells. The rapid expansion phase utilized closed G-Rex 500CS devices, yielding ~20 x 10^9 highly functional T-cells from a single vessel, improving safety and consistency.
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Publications

Tumor-infiltrating lymphocytes: Streamlining a complex manufacturing process

Reviews improvements to TIL manufacturing. Implementing G-Rex 100MCS devices for the Rapid Expansion Protocol (REP) reduced technical interventions, media usage, and contamination risk while maintaining comparable tumor specificity to flask/bag methods.
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