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Publications

Phase 1 Trial of Autologous CAR T Cells Targeting NKG2D Ligands in Patients with AML/MDS and Multiple Myeloma

This Phase 1 trial demonstrated the safety of NKG2D CAR-T cells in hematologic cancers. G-Rex were used for the ex vivo expansion of CAR-T cells during the 9-day manufacturing process, ensuring sufficient cell yield for infusion.
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Publications

BRAF targeting sensitizes resistant melanoma to cytotoxic T cells

The study investigates combining BRAF inhibitors with TIL therapy for resistant melanoma. In the associated clinical trial, patient TILs were rapidly expanded in G-Rex, facilitating the combination therapy approach.
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Publications

Enabling Large-Scale Ex Vivo Production of Megakaryocytes from CD34+ Cells Using Gas-Permeable Surfaces

This study evaluated G-Rex culture systems for producing megakaryocytes from CD34+ cells. G-Rex cultures with optimized seeding densities significantly increased the expansion of Mk progenitors and overall yield compared to standard tissue culture surfaces.
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Publications

Large-scale expansion of Vγ9Vδ2 T cells with engineered K562 feeder cells in G-Rex vessels and their use as chimeric antigen receptor– modified effector cells

A method to expand Vγ9Vδ2 T cells using engineered K562 aAPCs was optimized in G-Rex vessels. The G-Rex platform enabled the generation of large numbers of functional gamma-delta T cells suitable for CAR modification and allogeneic therapy.
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Publications

Identification of neoepitopes recognized by tumor-infiltrating lymphocytes (TILs) from patients with glioma

This study identified neoepitopes recognized by TILs from glioblastoma patients. G-Rex were used to expand TILs from tumor digests in the presence of OKT3 and irradiated feeder cells, providing sufficient cells for immune reactivity testing.
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Publications

Utilizing T-cell activation signals 1, 2 and 3 for tumor-infiltrating lymphocytes (TIL) expansion: the advantage over the sole use of interleukin-2 in cutaneous and uveal melanoma

A novel TIL expansion method combining IL-2, anti-4-1BB, and OKT3 was developed. Using small G-Rex 10 devices allowed for successful expansion from fewer tumor fragments and reduced culture time compared to standard methods, especially for uveal melanoma.
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Publications

TCRαβ/CD3 disruption enables CD3-specific antileukemic T cell immunotherapy

This paper reports the creation of anti-CD3 CAR-T cells (3CAR) using TALEN-mediated TCR disruption to avoid self-killing. G-Rex were used to scale up the production, allowing 3CAR T cells to expand 100-fold and self-enrich for the TCR-negative population.
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Publications

Efficient Enrichment of Gene-Modified Primary T Cells via CCR5-Targeted Integration of Mutant Dihydrofolate Reductase

The study demonstrates a method to enrich gene-edited CD4+ T cells using methotrexate selection. G-Rex 10 were employed for the long-term expansion of lentivirus-modified T cells, facilitating the generation of large numbers of selected cells.
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Publications

Manufacturing development and clinical production of NKG2D Chimeric Antigen Receptor-expressing T cells for autologous adoptive cell therapy

A 9-day manufacturing process for NKG2D CAR-T cells was developed for a Phase I trial. G-Rex 100 were utilized for the ex vivo expansion step, enabling the production of therapeutic doses of viable and functional CAR-T cells from patient material.
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Publications

Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells

This paper describes an improved method for generating CAR-T cells using the piggyBac transposon system. G-Rex10 culture were used for the expansion phase, contributing to the generation of clinically relevant numbers of CAR-T cells with a memory phenotype.
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Publications

CMV and EBV targets recognized by tumor-infiltrating B lymphocytes in pancreatic cancer and brain tumors

The study explores antibody responses from tumor-infiltrating B cells against viral antigens in cancer patients. G-Rex were used to expand tumor-infiltrating lymphocytes (TILs) from tumor tissue, facilitating the analysis of immune reactivity in the tumor microenvironment.
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Publications

Generation of Tumor-Specific (tg-TCR) T Cells Using CRISPR-Mediated In-Locus Insertion Combined with Endogenous TCR Knockout

This study demonstrates a method for generating tumor-specific TCR-T cells using CRISPR/Cas9 for endogenous TCR knockout and AAV for TCR insertion. G-Rex 24-well plates were utilized for T cell expansion, supporting cell viability and growth during the editing process.
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