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This study presents a therapeutic strategy combining CIK cells with the anti-CD20 antibody obinutuzumab. CIK cells were successfully expanded from patient PBMCs using G-Rex gas-permeable. The combination showed high cytotoxicity against B-cell malignancies in vitro and in vivo.

This Phase I trial assessed TIL therapy in metastatic NSCLC. Autologous TILs were expanded using a rapid expansion protocol in G-Rex 100 MCS. The treatment demonstrated manageable toxicity and induced durable complete responses in some patients.

This pilot study evaluated BKV-specific T cells (PyVST) for PML treatment. PyVSTs were generated from related donors and expanded in G-Rex tissue culture containers for 14 days. The therapy was safe, with some patients showing survival and viral load reduction.

This study established a method to expand SARS-CoV-2 specific T cells from convalescent donors. Using G-Rex culture vessels, including CS100M, the protocol achieved high yields of central memory T cells within 21 days for potential allogeneic therapy.

This study improved EBV-specific T cell manufacturing using GMP reagents and G-Rex. G-Rex100 yielded higher CD8+ T cell percentages and expansion compared to standard flasks. Detailed cytokine profiling characterized the product phenotype.

This study engineered CAR-NK cells to secrete IL-15, enhancing their anti-AML activity and persistence. G-Rex plates were utilized for a serial stimulation assay to evaluate NK cell proliferation and cytotoxicity over a 10-day period.

This paper details a manufacturing process for NKG2D CAR-T cells using piggyBac vectors and K562 aAPCs. It demonstrates robust expansion and cytotoxicity. While the specific protocol uses T75 flasks, it cites previous work utilizing G-Rex vessels for large-scale T cell expansion.

This study found that exercise mobilizes SARS-CoV-2 specific T-cells and antibodies. To assess T-cell function, SARS-CoV-2 virus-specific T-cells were expanded ex vivo from PBMCs using gas-permeable G-Rex devices for 7 days.

This article reports a superior method for expanding NK and CAR-NK cells using 221-mIL-21 feeder cells. The expanded cells showed enhanced cytotoxicity and metabolic function. G-Rex 6 multi-well plates were utilized for the culture and maintenance of the expanding NK cells.

This poster describes a modular, closed CAR-T manufacturing platform. It details expansion strategies using G-Rex 100MCS for low doses and a two-step process with G-Rex 500MCS for high doses, consistently achieving high cell viability and yields.

This study developed a GMP-compliant protocol for expanding CIK cells using G-Rex gas-permeable. G-Rex6 well plates yielded superior expansion and cytotoxicity compared to standard or G-Rex6M, achieving 400-fold expansion with minimal manipulation.

This study demonstrates that anti-CD147 CAR-T and CAR-NK cells effectively kill hepatocellular carcinoma cells. To minimize toxicity, a logic-gated GPC3-synNotch system was developed. Transduced NK cells were maintained and expanded in G-Rex 6 multi-well cell culture plates with IL-2.