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Virus-Specific T-Cell Therapy for the Management of Viral Infections in the Immunocompromised

A review article discussing VST therapies for immunocompromised patients. It mentions that simplified manufacturing processes use G-Rex culture devices for the selective activation and expansion of virus-reactive T cells.
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Publications

Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay

This paper evaluates a flow cytometric cytotoxicity assay for multiVSTs. It highlights that multiVSTs, expanded in G-Rex 5M devices for 14 days, show potent cytolytic activity against viral targets that is better detected by this new assay than by chromium release.
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Publications

Inosine Induces Stemness Features in CAR-T cells and Enhances Potency

The study shows that inosine induces stemness and metabolic reprogramming in CAR-T cells, improving potency. Clinical-scale manufacturing of GD2-CAR-T cells in inosine-containing media was performed using a semi-closed G-Rex system.
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Publications

Antiviral cellular therapy for enhancing T-cell reconstitution before or after hematopoietic stem cell transplantation (ACES): a two-arm, open label phase II interventional trial of pediatric patients with risk factor assessment

A Phase II trial evaluating third-party VSTs for refractory viral infections in pediatric patients post-HSCT or with IEI. VSTs were expanded using a rapid expansion protocol in G-Rex-10 for 10 days. Clinical response rate was 62%.
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Publications

Human 3D Ovarian Cancer Models Reveal Malignant Cell–Intrinsic and –Extrinsic Factors That Influence CAR T-cell Activity

The study uses 3D ovarian cancer models (spheroids, collagen gels, microfluidics) to study MUC1/TnMUC1 CAR T-cell activity. Resistance mechanisms and fibroblast interactions are explored. Transduced CAR T cells were cultured and expanded in G-Rex plates for 10 days.
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Publications

A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors

This study uses Quality-by-Design (QbD) and Design of Experiments (DOE) to optimize CAR-T expansion. Small-scale DOE studies were conducted in G-Rex 24-well plates to identify optimal parameters (single activation, short seed train), which were then translated to Ambr 250 bioreactors.
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Publications

Selection, engineering, and in vivo testing of a human leukocyte antigen–independent T-cell receptor recognizing human mesothelin

The study describes a mesothelin-specific HLA-independent TCR (HiT) that activates T cells and kills tumor cells in vitro and in vivo. Large-scale T-cell transduction and expansion were performed using G-Rex 6M plates.
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Publications

T antigen–specific CD8+ T cells associate with PD-1 blockade response in virus-positive Merkel cell carcinoma

The study analyzes T-Ag-reactive CD8+ T cells in Merkel cell carcinoma patients treated with anti-PD1. Responders showed broader T-cell recognition. T-Ag-specific T cells were expanded from PBMCs using artificial Ag-presenting scaffolds in G-Rex 24 well plates to enhance tumor killing.
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Publications

Human keratinocytes grown at a gas-permeable interface in vitro stratify correctly to generate engineered human epidermis

This study demonstrates that human keratinocytes cultured on a gas-permeable interface (GPI) stratify to form a mature epidermis, comparable to the air-liquid interface method. G-Rex 6 well plates were used as the GPI device for culturing the keratinocytes and inducing stratification.
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Publications

A rescue approach in refractory diffuse large B-cell lymphoma with obinutuzumab-redirected cytokine-induced killer cells: a first-in-human case report

A case report detailing the treatment of refractory DLBCL using CIK cells combined with obinutuzumab. The therapy achieved a very good partial remission without severe toxicity. CIK cells were produced and expanded for 14 days in G-Rex using a blinatumomab-based protocol.
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Publications

TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

The study develops allogeneic "Smart CAR T cells" using TALEN gene editing. These cells constitutively express a FAP CAR to target CAFs, which induces mesothelin CAR expression. This logic-gated approach improves solid tumor efficacy and safety. G-Rex 6M plates were used for the expansion of gene-edited T cells.
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Publications

Conserved allomorphs of MR1 drive the specificity of MR1-restricted TCRs

This study investigates the specificity of the MR1-restricted TCR from clone MC.7.G5. Results show recognition is restricted to the MR1*04 allomorph and is not cancer-specific, reacting to healthy cells expressing MR1*04. G-Rex systems were used to seed and expand CD3+ T cells during the transduction process.
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