Knowledge HQ

All
Publications

Human platelet lysate enhances in vivo activity of CAR-Vδ2 T cells by reducing cellular senescence and apoptosis

This study compares FBS and human platelet lysate (HPL) for expanding CAR-Vδ2 T cells. Cells expanded in G-Rex with HPL showed greater expansion and in vivo anti-tumor activity. HPL reduction of cellular senescence and apoptosis pathways contributed to improved persistence compared to FBS.
Download Now
Publications

Toll-like receptor 4 signaling activation domains promote CAR T cell function against solid tumors

This study investigated incorporating TLR4 signaling domains into CD19 CARs to improve efficacy against solid tumors. Primary T cells were transduced with CAR lentiviral vectors and expanded for 7-10 days in G-Rex six-well plates. The modified CAR-Ts exhibited enhanced cytotoxicity and cytokine secretion.
Download Now
Publications

Non-viral approaches in CAR-NK cell engineering: connecting natural killer cell biology and gene delivery

This review explores non-viral genetic modification methods for NK cells to produce off-the-shelf CAR-NK therapies. It highlights manufacturing strategies, noting that K562mbIL12-41BBL feeder cells facilitate large-scale expansion of peripheral blood NK cells in gas-permeable static cell culture (G-REX).
Download Now
Publications

Discovery and preclinical development of a therapeutically active nanobody-based chimeric antigen receptor targeting human CD22

This study details the development of a CD22-targeted nanobody-based CAR-T therapy. Primary human T cells were transduced with CD22sdCAR lentivirus and expanded in G-Rex 10M. The 1ug36-sdCAR candidate demonstrated significant in vivo therapeutic activity and favorable tissue specificity in xenograft models.
Download Now
Publications

In silico and pharmacological evaluation of GPR65 as a cancer immunotherapy target regulating T-cell functions

The study evaluates GPR65 as a target. TCR-T cells (NY-ESO1 or MAGE-A4) were produced in G-Rex 6 Well Plate systems.
Download Now
Publications

Engineering memory T cells as a platform for long-term enzyme replacement therapy in lysosomal storage disorders

This study engineers memory T cells to secrete IDUA for treating MPS I. Engineered Tm cells were cultured and expanded in 24-well G-Rex tissue culture plates.
Download Now
Publications

EphA3 CAR T cells are effective against glioblastoma in preclinical models

The study investigates EphA3-targeted CAR T cells for glioblastoma. T cells were transduced and expanded in G-Rex 24 well plates.
Download Now
Publications

Building a Better Defense: Expanding and Improving Natural Killer Cells for Adoptive Cell Therapy

A review on NK cell expansion and improvement. It mentions G-Rex plates as a culture material used in protocols for expanding NK cells, specifically citing studies where G-Rex 6-well plates or G-Rex 100M vessels were used.
Download Now
Publications

synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy

The study develops synNotch-programmed iPSC-NK cells to target CD155 and CD73 in glioblastoma. Cells were expanded for 2-3 weeks in G-Rex 24 well plates using NK Xpander medium.
Download Now
Publications

Expansion and Precise CRISPR-Cas9 Gene Repair of Autologous T-Memory Stem Cells from Patients with T-Cell Immunodeficiencies

This protocol details the expansion and gene repair of TSCM cells. It mentions that for scale-up and GMP-compliant experiments, electroporated TSCM can be expanded in G-Rex 100M gas-permeable culture vessels.
Download Now
Publications

Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method

The paper describes a feeder-free method to generate AlloCAR-NKT cells from HSPCs. G-Rex 6M well plates were used for the expansion stage (stage 4) of the NKT cell culture.
Download Now
Publications

Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes

The study introduces "NeoExpand" to selectively expand neoantigen-reactive TILs. It compares this to rapid expansion protocols (REP). G-Rex 24-well, 6-well, and 100 were used for the rapid expansion of T cells.
Download Now

ScaleReady is a joint venture between Wilson Wolf Manufacturing and Bio-Techne.