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Publications

Efficacy and safety of autologous tumor- infiltrating lymphocytes in recurrent or refractory ovarian cancer, colorectal cancer, and pancreatic ductal adenocarcinoma

This clinical trial assesses TIL therapy in refractory solid tumors. While no objective responses were seen, disease control was observed. The Rapid Expansion Protocol (REP) utilized G-Rex 10 and 100M to generate the large numbers of TILs required for patient infusion.
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Publications

Rapid parallel reconstruction and specificity screening of hundreds of T cell receptors

This protocol details a medium-throughput method to clone and screen TCRs against various antigens using combinatorial dye staining. G-Rex 24-well plates are recommended for expanding TCR-transduced T cells after Dynabead removal to ensure sufficient cell numbers for screening.
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Publications

Sequential Exposure to IL21 and IL15 During Human Natural Killer Cell Expansion Optimizes Yield and Function

The authors found that sequential stimulation with mbIL21 then mbIL15 feeder cells optimizes NK cell yield and function. Purified NK cells were co-cultured with irradiated K562 feeders in G-Rex 6-well plates, facilitating robust expansion for functional assessment.
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Publications

Enhancing CAR-T cell functionality in a patient-specific manner

This paper investigates how tuning T-cell stimulation dose using APC-mimetic scaffolds affects CAR-T phenotype and function across healthy and patient samples. 6-well G-Rex plates were used for the expansion of CAR-T cells, enabling the generation of diverse cell products for analysis.
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Publications

Targeting of intracellular oncoproteins with peptide-centric CARs

The study describes PC-CARs targeting the neuroblastoma oncoprotein PHOX2B presented on HLA. These CARs kill tumor cells across multiple HLA allotypes. Primary human T cells were activated and expanded in G-Rex system vessels (Wilson Wolf) during the retroviral and lentiviral transduction processes.
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Publications

Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models

This research combines affinity-tuned EpCAM/ICAM-1 CARs with NFAT-inducible IL-12 to enhance antitumor activity in solid tumors while minimizing toxicity. G-Rex 6M well plates were employed to expand the CAR-T cell products after lentiviral transduction.
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Publications

Integrome signatures of lentiviral gene therapy for SCID-X1 patients

The authors analyzed lentiviral vector integration sites in SCID-X1 patients and CD19-CAR T cells to identify safety and efficacy signatures. G-Rex 6-well and 100M plates were utilized for culturing transduced T cells, facilitating the generation of cell products for integrome analysis.
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Publications

Large-scale manufacturing of base-edited chimeric antigen receptor T cells

This study develops a protocol for manufacturing base-edited CAR-T cells using circular RNA (circRNA) to reduce costs and improve stability compared to linear mRNA. Large-scale production of PD-1 deficient CAR-T cells was achieved using 100M and six-well G-Rex vessels, demonstrating scalability.
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Publications

Intraventricular B7-H3 CAR T Cells for Diffuse Intrinsic Pontine Glioma: Preliminary First-in-Human Bioactivity and Safety

This phase I trial evaluates locoregional B7-H3 CAR-T cells for DIPG. The clinical CAR-T cell products were manufactured using G-Rex 100MCS vessels, incorporating a methotrexate selection step to enrich for modified cells.
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Publications

Feasibility and Safety of Personalized, Multi-Target, Adoptive Cell Therapy (IMA101): First-In-Human Clinical Trial in Patients with Advanced Metastatic Cancer

The study reports on the IMA101 clinical trial. Endogenous multi-target T cells were primed and expanded using G-Rex 100MCS and G-Rex 100M vessels in a rapid expansion protocol, enabling the generation of large cell numbers for patient infusion.
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Publications

Lentiviral vectors for inducible, transactivator-free advanced therapy medicinal products: Application to CAR-T cells

This study develops Dox-inducible lentiviral vectors for CAR-T cells. G-Rex plates were used for the production of monoclonal antibodies from hybridomas and for expanding transduced primary T cells in the absence of Dox prior to in vivo experiments.
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Publications

Rapid and sustained T cell-based immunotherapy against invasive fungal disease via a combined two step procedure

The paper describes a manufacturing strategy for Aspergillus-specific T cells (ATCs). A short-term expansion (STE) protocol using G-Rex devices allowed for the generation of clinically relevant numbers of functional ATCs within 12 days.
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