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Extracellular Vesicles from Immune Cells: A Biomedical Perspective

This review explores the role of small extracellular vesicles (sEVs) derived from various immune cells. It highlights a protocol using G-Rex to produce and isolate large amounts of human NK cell-derived sEVs, demonstrating their potential for clinical application in cancer therapy.
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Publications

Process engineering of natural killer cell-based immunotherapy

This review discusses the biomanufacturing of NK cell therapies, covering cell sources, expansion methods, and process control. It identifies G-Rex as a commonly used culture system for clinical-scale NK cell production due to their gas-permeable membranes, facilitating efficient cell expansion.
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Publications

Activated T cell therapy targeting glioblastoma cancer stem cells

The study develops an activated T cell (ATC) therapy targeting glioblastoma cancer stem cells. It compares antigen sources, finding synthetic peptide pools most effective. T cells were co-cultured with antigen-loaded dendritic cells in G-Rex 100 containers for activation and rapid proliferation over 12 days.
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Publications

Persistence of spike-specific immune responses in BNT162b2-vaccinated donors and generation of rapid ex-vivo T cells expansion protocol for adoptive immunotherapy: A pilot study

This study characterizes humoral and T cell responses to the BNT162b2 vaccine. It establishes a rapid 11-day ex-vivo expansion protocol for spike-specific T cells using G-Rex 10 devices, demonstrating significant fold expansion and functional enhancement for potential adoptive immunotherapy.
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Publications

Identification of a small molecule for enhancing lentiviral transduction of T cells

High-throughput screening identified a small molecule, GSK2622391, that enhances lentiviral transduction efficiency in T cells. The compound improves vector copy number without negatively impacting cell viability or function. G-Rex plates were used for T cell expansion during small-scale validation experiments.
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Publications

Metabolic reprogramming via an engineered PGC-1α improves human chimeric antigen receptor T-cell therapy against solid tumors

The study shows that overexpression of an engineered PGC-1α improves the metabolic fitness and anti-tumor efficacy of CAR-T cells. During in vitro culture, cells were transferred to G-Rex 6M Well Plates for rapid expansion.
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Publications

Current status of red blood cell manufacturing in 3D culture and bioreactors

This review covers various bioreactor systems for red blood cell production. It specifically references the use of G-Rex for the expansion of peripheral blood mononuclear cells (PB MNCs) in its summary of 3D scaffolds and bioreactors.
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Publications

Signaling via a CD28/CD40 chimeric costimulatory antigen receptor (CoStAR™), targeting folate receptor alpha, enhances T cell activity and augments tumor reactivity of tumor infiltrating lymphocytes

The study demonstrates that CoStAR, a chimeric costimulatory receptor, enhances the effector function of T cells and TILs. Transgene-expressing cells and TILs were expanded using G-Rex plates, including G-Rex 6M, as part of the rapid expansion protocol.
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Publications

CAR T-Cell therapy for the management of mantle cell lymphoma

This review summarizes the current landscape of CAR T-cell therapy for mantle cell lymphoma. It discusses manufacturing processes, noting that selection, activation, transduction, and expansion steps are often performed in systems such as G-Rex bottles.
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Publications

The CD58-CD2 axis is co-regulated with PD-L1 via CMTM6 and shapes anti-tumor immunity

This research explores the mechanisms of immune evasion related to CD58 loss and PD-L1 upregulation. Patient-derived TILs and CRISPR-engineered T cells used in the experiments were expanded using G-Rex 10 and G-Rex 100 bottles.
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Publications

CAR-NK Cells Generated with mRNA-LNPs Kill Tumor Target Cells In Vitro and In Vivo

The authors demonstrate a method to generate CAR-NK cells using mRNA-LNP transfection. The NK cells used in the study were cultured and expanded in G-Rex 6-well and 24-well plates.
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Publications

Targeting the CDK6-PTPs axis enhances the efficacy of T cell killing and adoptive T cell immunotherapy

This study investigates the role of CDK6 and PTPs in regulating T cell activity and immunotherapy resistance. CD19-CAR T cells used in the study were expanded in G-Rex 10 and G-Rex 100 to generate sufficient cell numbers.
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