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The study investigates T cell responses to SARS-CoV-2 and endemic coronaviruses. It describes a protocol to generate universal multi-coronavirus specific T cells (MCVSTs) by expanding PBMCs in G-Rex gas-permeable containers, yielding robust cell numbers for potential adoptive immunotherapy.

This paper describes a pooled screening strategy for CAR domain combinations using DNA barcoding. G-Rex plates were utilized for co-culturing transduced T cells with tumor cells during repeated antigen stimulation assays to identify CAR constructs with superior expansion and proliferation.

The study evaluates an oncolytic adenovirus expressing vIL-2 to boost TIL therapy for ovarian cancer. Autologous TILs were expanded from patient tumor fragments using six-well G-Rex plates as part of the 'young' TILs expansion protocol.

This study demonstrates that an oncolytic adenovirus encoding vIL-2 enhances NK cell cytotoxicity against ovarian cancer. Patient PBMCs used for humanized mouse models were expanded and rested using 6-well G-Rex plates with TILs media.

The study analyses neoantigen-specific T cells in melanoma patients. Isolated neoTCRs were engineered into donor T cells, which were expanded in 24-well G-Rex plates. The expanded cells were used to validate TCR specificity and cytotoxicity against autologous tumor cell lines.

The study isolates neutralizing human mAbs against Sosuga virus from a survivor. Hybridoma cell lines secreting these mAbs were expanded in 6-well G-Rex plates to produce sufficient antibodies for purification and subsequent functional characterization.

This study evaluates anti-CD20 pre-treatment for enhancing SIV-specific CAR-T cell therapy in macaques. CAR-T cells were manufactured using G-Rex 6-well plates for expansion. While anti-CD20 treatment increased initial CAR-T expansion, the cells failed to persist long-term, and viral loads were not significantly affected.

The paper presents a low-cost, point-of-care CAR-T manufacturing process. It uses the Sepax C-Pro for cell processing and G-Rex devices for cell expansion. The study demonstrates that this simplified workflow produces highly functional CAR-T cells, comparable to standard methods, supporting decentralized production.

This review explores the role of small extracellular vesicles (sEVs) derived from various immune cells. It highlights a protocol using G-Rex to produce and isolate large amounts of human NK cell-derived sEVs, demonstrating their potential for clinical application in cancer therapy.

This review discusses the biomanufacturing of NK cell therapies, covering cell sources, expansion methods, and process control. It identifies G-Rex as a commonly used culture system for clinical-scale NK cell production due to their gas-permeable membranes, facilitating efficient cell expansion.

The study develops an activated T cell (ATC) therapy targeting glioblastoma cancer stem cells. It compares antigen sources, finding synthetic peptide pools most effective. T cells were co-cultured with antigen-loaded dendritic cells in G-Rex 100 containers for activation and rapid proliferation over 12 days.

This study characterizes humoral and T cell responses to the BNT162b2 vaccine. It establishes a rapid 11-day ex-vivo expansion protocol for spike-specific T cells using G-Rex 10 devices, demonstrating significant fold expansion and functional enhancement for potential adoptive immunotherapy.