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The authors developed a scalable process for expanding NK and CAR-NK cells using G-Rex® 100M closed-system. The study showed that cells expanded in G-Rex® 100M retained similar phenotypes and cytotoxicity to those grown in G-Rex® 6-well plates.

This study demonstrates that S309-CAR-NK cells can bind to SARS-CoV-2 variants and reduce viral loads in humanized ACE2-NSG mice. Primary NK cell expansion and CAR-NK cell maintenance were performed using G-Rex® wells.

This study compares CAR-T expansion in Prodigy, Xuri, G-Rex®, and bags. G-Rex® (500M) cultures yielded effector memory-enriched cells. Hypoxic culture in G-Rex® increased the proportion of naïve/stem central memory-like cells, suggesting oxygenation is a key factor in T cell differentiation during manufacturing.

This study introduces the YT-rCTL/KFRET system for rapid TCR functional profiling. YT-Indy based effector cell lines were subcultured in 24-well G-Rex® plates. The platform allows for the recombinant expression of TCRs and antigens to assess cytotoxic responses via a granzyme-activatable reporter.

This study demonstrates that proteasome inhibitors sensitize AML cells to NK cell killing. NK cells were activated by co-culture with irradiated feeder cells in G-Rex® 10 vessels. The combination of bortezomib pre-treatment and CAR-NK infusion significantly delayed tumor growth and prolonged survival in xenograft models.

This study presents IL-2-armored GPS molecules that selectively expand and redirect CMV-specific CD8+ T cells. Expansion assays were conducted in 6-well and 24-well G-Rex® plates. The approach induced potent cytotoxicity against cancer cells with a reduced cytokine release profile compared to conventional TCEs.

This study identifies placental circulating T cells as a superior source for allogeneic CAR-T therapy. T cells were activated and expanded in G-Rex® plates. The resulting CAR-T cells retained stemness markers, exhibited a favorable cytokine profile, and demonstrated durable in vivo efficacy against lymphoma.

This study optimizes CAR-NK manufacturing using primary NK cells. Expansion was performed in 48-well G-Rex® plates using irradiated feeder cells. Adult PB-NK cells showed superior yield and cytotoxicity. The protocol achieved high transduction efficiency and scalable expansion suitable for clinical use.

This study presents a method to generate universal CAR-T cells with a defined memory phenotype by editing TRAC and B2M genes. Cells were cultured in G-Rex® 24-well plates. The safety of the editing was confirmed via CRISPRroots analysis, and the cells showed effective anti-tumor activity in vitro.

This report describes the operation of Greek public UCB banks. It highlights initiatives to reuse non-transplantable units for cell therapy. Specifically, it details the expansion of immunomagnetically selected CD34+ cells in G-Rex® to differentiate into dendritic cells for generating leukemia-specific T cells.

This study examines Tmod blocker function targeting non-HLA antigens, finding that blocker efficacy depends on antigen height. Primary human T cells were transduced with Tmod constructs and grown in G-Rex® plates. The findings established geometric design principles to optimize Tmod selectivity and sensitivity.

This study investigates the therapeutic effects of Aβ-specific Tregs in Alzheimer's disease mice. Purified CD4+CD25+ Tregs were expanded in G-Rex® 24-well plates with CD3/28 MACSiBead particles and IL-2. Adoptive transfer improved cognitive function and reduced Aβ and pTAU accumulation in the hippocampus.