Knowledge HQ

Publications
Publications

Development of an anti-CAR antibody response in SIV-infected rhesus macaques treated with CD4-MBL CAR/CXCR5 T cells

This study monitored anti-CAR antibody responses in rhesus macaques infused with CD4-MBL CAR/CXCR5 T cells. The therapeutic cells were expanded in G-Rex 6-well plates prior to infusion. All treated animals developed anti-CAR antibodies, which correlated with limited in vivo persistence of the CAR T cells.
Download Now
Publications

AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines

This paper presents AsCas12a Ultra, an engineered nuclease with high editing efficiency. G-Rex technology was used to expand human CD3+ T cells and activate NK cells during the editing workflows. The nuclease enabled robust multiplex editing and transgene knock-in in various primary cell types.
Download Now
Publications

Rapid Generation of TCR and CD8ab Transgenic Virus Specific T Cells for Immunotherapy of Leukemia

The authors describe a method to generate TCR and CD8ab transgenic VSTs using IFN-g cytokine capture. G-Rex devices were utilized for the second stimulation expansion phase, yielding high numbers of polyfunctional T cells with simultaneous anti-viral and anti-tumor activity in a shortened timeframe.
Download Now
Publications

Using Allogeneic, Off-the-Shelf, Sars-Cov-2-Specific T Cells to Treat High Risk Patients with COVID-19

This study explores generating banked SARS-CoV-2-specific virus-specific T cells (VSTs) for immunocompromised patients. The manufacturing process utilized G-Rex devices with activating cytokines to produce Th1-polarized, polyfunctional T cells capable of selectively killing viral antigen-expressing targets.
Download Now
Publications

Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive Cancers

The authors engineered high-potency CARs targeting HPV E6 peptide-MHC complexes. Primary T cells transduced with these CARs were expanded in 24-well G-Rex plates supplemented with IL-2. The optimized CARs showed comparable sensitivity and selectivity to clinical TCRs in cytotoxicity assays.
Download Now
Publications

GMP-Compliant Universal Antigen Presenting Cells (uAPC) Promote the Metabolic Fitness and Antitumor Activity of Armored Cord Blood CAR-NK Cells

This study describes the use of universal antigen presenting cells (uAPC) to expand cord blood CAR-NK cells. G-Rex (M100 series) were used for GMP-grade expansion, enabling a closed system with optimal gas exchange. The process achieved robust expansion and enhanced antitumor activity of the NK cells.
Download Now
Publications

CAR-NK Cells Effectively Target SARS-CoV-2-Spike-Expressing Cell Lines In Vitro

The authors developed CAR-NK cells using the S309 antibody to target SARS-CoV-2. Primary S309-CAR-NK cells were expanded in G-Rex plates for 21 days following transduction. These cells demonstrated effective binding and killing of spike-protein expressing target cells in vitro.
Download Now
Publications

Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids

This study reports the spontaneous generation of hyaline cartilage in hiPSC-derived multi-tissue organoids (MTOs) using a xeno- and feeder-free protocol. G-Rex100 were used to culture organoids for up to 30 weeks, overcoming hypoxia limitations. RNA-seq links cartilage formation to BMP signaling.
Download Now
Publications

Spatio‑temporal biodistribution of 89Zr‑oxine labeled huLym‑1‑A‑BB3z‑CAR T‑cells by PET imaging in a preclinical tumor model

The authors tracked 89Zr-oxine labeled CAR-T cells targeting lymphoma using PET/MRI. Primary T cells were transduced and expanded in G-Rex plates. The study demonstrated dose-dependent tumor accumulation and transient lung retention followed by liver/spleen migration.
Download Now
Publications

Inhibition of MEK pathway enhances the antitumor efficacy of chimeric antigen receptor T cells against neuroblastoma

This study investigated combining GD2-CAR T cells with the MEK inhibitor trametinib for neuroblastoma. PiggyBac-generated CAR-T cells were expanded in G-Rex 6-well plates. The combination improved antitumor efficacy in vivo, overcoming MEK inhibitor-induced T cell suppression seen in vitro.
Download Now
Publications

DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system

The authors developed DL4-conjugated microbeads to induce T cell differentiation from CD34+ stem cells without stromal cells. The system was adaptable to G-Rex plates for scalable production. It successfully generated proT cells capable of thymic engraftment and maturation.
Download Now
Publications

Virus-specific T cells for adenovirus infection after stem cell transplantation are highly effective and class II HLA restricted

This phase 1/2 trial evaluated virus-specific T cells (VSTs) for adenovirus infection post-transplant. VSTs were manufactured using G-Rex 10-M devices for 11-12 days. The therapy was highly effective (81% response rate), and efficacy correlated with Class II HLA restriction.
Download Now

ScaleReady is a joint venture between Wilson Wolf Manufacturing and Bio-Techne.