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Publications

Spatio‑temporal biodistribution of 89Zr‑oxine labeled huLym‑1‑A‑BB3z‑CAR T‑cells by PET imaging in a preclinical tumor model

The authors tracked 89Zr-oxine labeled CAR-T cells targeting lymphoma using PET/MRI. Primary T cells were transduced and expanded in G-Rex plates. The study demonstrated dose-dependent tumor accumulation and transient lung retention followed by liver/spleen migration.
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Publications

Inhibition of MEK pathway enhances the antitumor efficacy of chimeric antigen receptor T cells against neuroblastoma

This study investigated combining GD2-CAR T cells with the MEK inhibitor trametinib for neuroblastoma. PiggyBac-generated CAR-T cells were expanded in G-Rex 6-well plates. The combination improved antitumor efficacy in vivo, overcoming MEK inhibitor-induced T cell suppression seen in vitro.
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Publications

DL4-μbeads induce T cell lineage differentiation from stem cells in a stromal cell-free system

The authors developed DL4-conjugated microbeads to induce T cell differentiation from CD34+ stem cells without stromal cells. The system was adaptable to G-Rex plates for scalable production. It successfully generated proT cells capable of thymic engraftment and maturation.
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Publications

Virus-specific T cells for adenovirus infection after stem cell transplantation are highly effective and class II HLA restricted

This phase 1/2 trial evaluated virus-specific T cells (VSTs) for adenovirus infection post-transplant. VSTs were manufactured using G-Rex 10-M devices for 11-12 days. The therapy was highly effective (81% response rate), and efficacy correlated with Class II HLA restriction.
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Publications

Virus Induced Lymphocytes (VIL) as a novel viral antigen‑specific T cell therapy for COVID‑19 and potential future pandemics

The authors developed Virus Induced Lymphocytes (VIL) for COVID-19 using artificial APCs (VIPR particles). G-Rex plates were used to facilitate rapid expansion (7 days) of SARS-CoV-2 specific T cells. The resulting VILs showed a polyfunctional cytokine profile and effector memory phenotype.
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Publications

Immunogenic chemotherapy enhances recruitment of CAR-T cells to lung tumors and improves anti-tumor efficacy when combined with checkpoint blockade

This study used a KPROR1 lung cancer model to show that immunogenic chemotherapy (Ox/Cy) enhances ROR1 CAR-T cell infiltration. Clinical human ROR1 CAR-T cells were expanded in G-Rex. The combination of Ox/Cy, CAR-T, and anti-PD-L1 improved survival.
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Publications

Oncolytic adeno-immunotherapy modulates the immune system enabling CAR T-cells to cure pancreatic tumors

The study combines oncolytic adenovirus (CAdTrio) with HER2-CAR T cells to treat pancreatic cancer. NK cells, expanded in G-Rex devices with feeder cells, were also used in experiments. The combination therapy modulated the tumor microenvironment and improved CAR-T infiltration and efficacy.
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Publications

CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma

This paper outlines the CARAMBA clinical trial for SLAMF7 CAR-T cells generated via Sleeping Beauty transposon. The GMP manufacturing process utilizes G-Rex culture for the expansion of CAR-T cells over 12 days. This is the first clinical trial using advanced Sleeping Beauty technology.
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Publications

Re-examination of MAGE-A3 as a T-cell Therapeutic Target

The authors investigate MAGE-A3 as a therapeutic target, proposing EPS8L2 as the source of prior TCR-T neurotoxicity. They developed MAGE-A3-directed CARs and TCRs, expanding transduced primary T cells in G-Rex plates. The CARs showed improved selectivity against the off-target peptide.
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Publications

Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity

Knockout of DNMT3A in CAR-T cells prevents exhaustion and enhances antitumor activity. G-Rex-6M culture plates were used for the expansion of gene-edited CAR-T cells. The modified cells retained a stem-like epigenetic program and showed superior tumor control in vivo.
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Publications

Preclinical Evaluation of 89Zr-Df-IAB22M2C PET as an Imaging Biomarker for the Development of the GUCY2C-CD3 Bispecific PF-07062119 as a T Cell Engaging Therapy

This study evaluates 89Zr-Df-IAB22M2C PET imaging to track CD8+ T cells in a colorectal cancer model treated with a GUCY2C-CD3 bispecific antibody. G-Rex devices were used to expand human T cells prior to adoptive transfer. The tracer effectively monitored dose-dependent T cell infiltration.
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Publications

RNA-Seq Analysis Reveals CCR5 as a Key Target for CRISPR Gene Editing to Regulate In Vivo NK Cell Trafficking

RNA-seq revealed that ex vivo expansion alters chemokine receptor expression in NK cells, notably upregulating CCR5. G-Rex were used to expand NK cells with feeder cells. CRISPR-mediated CCR5 disruption reduced liver trafficking and increased NK cell presence in circulation in mice.
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