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Publications
Publications

Challenges and advantages of cell therapy manufacturing under Good Manufacturing Practices within the hospital setting

This review discusses establishing academic GMP facilities for cell therapy. It highlights the use of closed systems like G-Rex (referenced via Harari et al.) to improve efficiency and compliance. The article emphasizes the benefits of hospital-based manufacturing for personalized therapies like TILs.
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Publications

Development of an optimized closed and semi-automatic protocol for Good Manufacturing Practice manufacturing of tumor-infiltrating lymphocytes in a hospital environment

The authors developed a GMP-compliant TIL expansion protocol using G-Rex and the Xuri bioreactor. Pre-REP expansion in G-Rex resulted in higher cell numbers compared to 24-well plates. The closed-system process reduced operator time and produced functional TILs suitable for clinical trials.
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Publications

Characterization of a Cytomegalovirus-Specific T Lymphocyte Product Obtained Through a Rapid and Scalable Production Process for Use in Adoptive Immunotherapy

This study characterizes CMV-specific T lymphocytes (VSTs) expanded rapidly for adoptive immunotherapy. Using G-Rex technology with IL-2, IL-7, and IL-15, researchers achieved a scalable process yielding functional, cytotoxic T cells. The method ensures high specificity and safety for treating viral infections.
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Publications

Photodepletion with 2-Se-Cl prevents lethal graft-versus-host disease while preserving antitumor immunity

This research demonstrates that photodepletion with 2-Se-Cl selectively removes alloreactive T cells to prevent GVHD without compromising antitumor immunity. G-Rex devices were utilized for the initial co-culture and priming of splenocytes before the photodepletion step.
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Publications

Optimization and Scale-up of Treg Expansion from CD4+ Cells in G-Rex6M plates

This poster details the optimization of Treg expansion using Cloudz™ Treg CD3/CD28 microspheres. The method achieved high purity and cell numbers. Scalability was demonstrated using G-Rex6M plates, which utilize a gas-permeable membrane to support efficient cell growth in serum-free media.
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Publications

Inhibition of MICA and MICB Shedding Elicits NK cell–mediated Immunity against Tumors Resistant to Cytotoxic T cells

The study demonstrates that blocking MICA/B shedding enables NK cells to target tumors with MHC-I or IFN-gamma pathway mutations. Human NK cells were expanded in vitro using G-Rex 6-well plates. This therapeutic strategy effectively reduced metastases in humanized mouse models.
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Publications

Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting Cells

This study develops renewable artificial antigen presenting cells (aAPCs) for T cell expansion and transduction. Using G-Rex , aAPCs supported efficient production of CAR T cells and TILs with less exhaustion and higher potency than bead-based methods, offering a cost-effective manufacturing alternative.
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Publications

Detection of response to tumor microenvironment–targeted cellular immunotherapy using nano-radiomics

This study explores nano-radiomics to assess response to MDSC-directed NK cell therapy in solid tumors. Human NK cells were activated and expanded in G-Rex devices for in vivo testing. Radiomic analysis of CT images successfully identified treatment responses that conventional tumor metrics failed to detect.
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Publications

Production of Lentiviral Vectors Using Suspension Cells Grown in Serum-free Media

The authors developed a scalable, serum-free process for lentiviral vector production using suspension-adapted SJ293TS cells. The vectors efficiently transduced human T cells and CD34+ cells. G-Rex plates were employed to expand the transduced T cells for assessing chimeric antigen receptor expression and titer.
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Publications

Head- to- head comparison of in- house produced CD19 CAR- T cell in ALL and NHL patients

This study compares in-house produced CD19 CAR-T cells for ALL and NHL. Cells from ALL patients expanded better. Production utilized G-Rex100 , enabling rapid generation of therapeutic doses. The study highlights phenotypic differences based on the patient's disease.
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Publications

Rapidly expanded partially HLA DRB1–matched fungus-specific T cells mediate in vitro and in vivo antifungal activity

The authors describe a GMP-compliant method to rapidly expand fungus-specific T cells using CD137 selection and short-term culture. G-Rex cell culture plates were used for the in vitro expansion of the enriched CD137+ population. The resulting T cells demonstrated potent antifungal activity against Aspergillus.
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Publications

Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags

This study compares monocyte-derived dendritic cell (Mo-DC) production in fluorinated ethylene propylene bags versus polystyrene plates. Mo-DCs generated in bags showed comparable phenotype and ability to activate T cells. G-Rex were utilized for the T cell co-culture steps to assess activation potency.
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