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This research demonstrates that photodepletion with 2-Se-Cl selectively removes alloreactive T cells to prevent GVHD without compromising antitumor immunity. G-Rex devices were utilized for the initial co-culture and priming of splenocytes before the photodepletion step.

This poster details the optimization of Treg expansion using Cloudz™ Treg CD3/CD28 microspheres. The method achieved high purity and cell numbers. Scalability was demonstrated using G-Rex6M plates, which utilize a gas-permeable membrane to support efficient cell growth in serum-free media.

The study demonstrates that blocking MICA/B shedding enables NK cells to target tumors with MHC-I or IFN-gamma pathway mutations. Human NK cells were expanded in vitro using G-Rex 6-well plates. This therapeutic strategy effectively reduced metastases in humanized mouse models.

This study develops renewable artificial antigen presenting cells (aAPCs) for T cell expansion and transduction. Using G-Rex , aAPCs supported efficient production of CAR T cells and TILs with less exhaustion and higher potency than bead-based methods, offering a cost-effective manufacturing alternative.

This study explores nano-radiomics to assess response to MDSC-directed NK cell therapy in solid tumors. Human NK cells were activated and expanded in G-Rex devices for in vivo testing. Radiomic analysis of CT images successfully identified treatment responses that conventional tumor metrics failed to detect.

The authors developed a scalable, serum-free process for lentiviral vector production using suspension-adapted SJ293TS cells. The vectors efficiently transduced human T cells and CD34+ cells. G-Rex plates were employed to expand the transduced T cells for assessing chimeric antigen receptor expression and titer.

This study compares in-house produced CD19 CAR-T cells for ALL and NHL. Cells from ALL patients expanded better. Production utilized G-Rex100 , enabling rapid generation of therapeutic doses. The study highlights phenotypic differences based on the patient's disease.

The authors describe a GMP-compliant method to rapidly expand fungus-specific T cells using CD137 selection and short-term culture. G-Rex cell culture plates were used for the in vitro expansion of the enriched CD137+ population. The resulting T cells demonstrated potent antifungal activity against Aspergillus.

This study compares monocyte-derived dendritic cell (Mo-DC) production in fluorinated ethylene propylene bags versus polystyrene plates. Mo-DCs generated in bags showed comparable phenotype and ability to activate T cells. G-Rex were utilized for the T cell co-culture steps to assess activation potency.

Evaluated TIL ACT for ovarian cancer. TILs were expanded using a 'TIL 3.0' method combining IL-2 with agonistic 4-1BB and anti-CD3 antibodies in G-Rex 10. This method significantly increased CD8+ TIL yield and success rates compared to IL-2 alone, producing tumor-reactive T cells in 2-3 weeks.

Production of a third-party CMV-specific T cell (CMVST) bank. Cells were expanded using G-Rex 5 and G-Rex 100M. The bank successfully provided matched lines for 96.6% of screened patients with 100% response rate in treated patients.

Study improving Vg9Vd2 T cell responses using NKG2D RNA CARs. Cells were expanded using K562 aAPCs in G-Rex vessels before electroporation. CAR-modified cells showed enhanced cytotoxicity against solid tumors in vitro and in vivo.