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Publications
Publications

A Phase I Clinical Trial with Ex Vivo Expanded Recipient Regulatory T cells in Living Donor Kidney Transplants

This Phase I trial assessed the safety of infusing autologous expanded Tregs into kidney transplant recipients to induce tolerance. G-Rex were used to expand Tregs, achieving robust cell numbers and meeting all clinical release criteria without adverse events.
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Publications

In Vitro Expansion of Anti-viral T Cells from Cord Blood by Accelerated Co-cultured Dendritic Cells

A method to generate virus-reactive T cells from cord blood using accelerated co-cultured dendritic cells (acDC). The use of G-Rex 10 significantly increased the yield of antigen-reactive CD8+ T cells compared to standard plates, enabling therapeutic dosing.
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Publications

Tumor-infiltrating lymphocytes: Streamlining a complex manufacturing process

Reviews improvements to TIL manufacturing. Implementing G-Rex 100MCS devices for the Rapid Expansion Protocol (REP) reduced technical interventions, media usage, and contamination risk while maintaining comparable tumor specificity to flask/bag methods.
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Publications

Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules

Evaluated a closed-system manufacturing process for TCR-transduced T-cells. The rapid expansion phase utilized closed G-Rex 500CS devices, yielding ~20 x 10^9 highly functional T-cells from a single vessel, improving safety and consistency.
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Publications

Long Terminal Repeat CRISPR-CAR-Coupled “Universal” T Cells Mediate Potent Anti-leukemic Effects

Developed "universal" CAR T cells using a lentiviral vector coupling CAR expression with CRISPR/Cas9 gene editing. G-Rex were used for conventional control cultures to compare against the automated CliniMACS Prodigy system.
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Publications

Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells

A scalable protocol for producing red blood cells from adult PBMCs was developed. The G-Rex allowed for massive expansion (3x10^6 fold) of erythroblasts and supported efficient differentiation and enucleation, comparable to standard culture dishes.
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Publications

Bags versus: a comparison of cell culture systems for the production of dendritic cell–based immunotherapies

Review comparing cell culture systems (bags vs flasks) for dendritic cell production. Discusses gas permeability and materials. References Wilson Wolf patents related to gas-permeable devices as examples of improved culture methods utilizing silicone rubber membranes.
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Publications

Prospective analysis of adoptive TIL therapy in patients with metastatic melanoma: response, impact of anti-CTLA4, and biomarkers to predict clinical outcome

Prospective analysis of TIL therapy in metastatic melanoma. The Rapid Expansion Protocol (REP) was performed using G-Rex 100M, contributing to the generation of infusion products. High TIL numbers were associated with response.
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Publications

CAR T cell therapy for breast cancer: harnessing the tumor milieu to drive T cell activation

Generated MUC1-specific CAR T cells co-expressing an inverted cytokine receptor (4/7ICR) for breast cancer. Co-culture experiments to monitor anti-tumor activity were conducted in 6-well G-Rex devices using a 3D algimatrix bioscaffold.
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Publications

Overcoming Target Driven Fratricide for T Cell Therapy

Investigated methods to overcome fratricide in NKG2D-CAR T cell production. The manufacturing process included an expansion phase in G-Rex containers, which was part of the upscaling strategy to produce sufficient cell yields.
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Publications

Lym-1 Chimeric Antigen Receptor T Cells Exhibit Potent Anti-Tumor Effects against B-Cell Lymphoma

Evaluated Lym-1 CAR T cells against B-cell lymphoma. Primary T cells were transduced and expanded in 24-well G-Rex plates. The cells showed potent anti-tumor effects in vitro and in vivo against Raji xenografts.
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Publications

4-1BB agonist focuses CD8+ tumor-infiltrating T-cell growth into a distinct repertoire capable of tumor recognition in pancreatic cancer

Study on 4-1BB agonist enhancing CD8+ TIL growth from pancreatic ductal adenocarcinoma (PDAC). The Rapid Expansion Protocol (REP) was performed in G-Rex 10 devices, enabling the exponential growth of sorted CD8+ and bulk TIL populations for functional assays.
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