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This study evaluated G-Rex culture systems for producing megakaryocytes from CD34+ cells. G-Rex cultures with optimized seeding densities significantly increased the expansion of Mk progenitors and overall yield compared to standard tissue culture surfaces.

This study identified neoepitopes recognized by TILs from glioblastoma patients. G-Rex were used to expand TILs from tumor digests in the presence of OKT3 and irradiated feeder cells, providing sufficient cells for immune reactivity testing.

This paper reports the creation of anti-CD3 CAR-T cells (3CAR) using TALEN-mediated TCR disruption to avoid self-killing. G-Rex were used to scale up the production, allowing 3CAR T cells to expand 100-fold and self-enrich for the TCR-negative population.

A novel TIL expansion method combining IL-2, anti-4-1BB, and OKT3 was developed. Using small G-Rex 10 devices allowed for successful expansion from fewer tumor fragments and reduced culture time compared to standard methods, especially for uveal melanoma.

A 9-day manufacturing process for NKG2D CAR-T cells was developed for a Phase I trial. G-Rex 100 were utilized for the ex vivo expansion step, enabling the production of therapeutic doses of viable and functional CAR-T cells from patient material.

The study demonstrates a method to enrich gene-edited CD4+ T cells using methotrexate selection. G-Rex 10 were employed for the long-term expansion of lentivirus-modified T cells, facilitating the generation of large numbers of selected cells.

The study explores antibody responses from tumor-infiltrating B cells against viral antigens in cancer patients. G-Rex were used to expand tumor-infiltrating lymphocytes (TILs) from tumor tissue, facilitating the analysis of immune reactivity in the tumor microenvironment.

This paper describes an improved method for generating CAR-T cells using the piggyBac transposon system. G-Rex10 culture were used for the expansion phase, contributing to the generation of clinically relevant numbers of CAR-T cells with a memory phenotype.

This study demonstrates a method for generating tumor-specific TCR-T cells using CRISPR/Cas9 for endogenous TCR knockout and AAV for TCR insertion. G-Rex 24-well plates were utilized for T cell expansion, supporting cell viability and growth during the editing process.

This Phase I trial assessed the safety of infusing autologous expanded Tregs into kidney transplant recipients to induce tolerance. G-Rex were used to expand Tregs, achieving robust cell numbers and meeting all clinical release criteria without adverse events.

A method to generate virus-reactive T cells from cord blood using accelerated co-cultured dendritic cells (acDC). The use of G-Rex 10 significantly increased the yield of antigen-reactive CD8+ T cells compared to standard plates, enabling therapeutic dosing.

Reviews improvements to TIL manufacturing. Implementing G-Rex 100MCS devices for the Rapid Expansion Protocol (REP) reduced technical interventions, media usage, and contamination risk while maintaining comparable tumor specificity to flask/bag methods.