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Details the GMP manufacturing process for trivirus-specific CTLs (CMV, EBV, Adv). The protocol allows for stimulation and expansion in either 24-well plates or G-Rex devices (specifically G-Rex40 and G-Rex500). G-Rex is noted for supporting high-density cell growth and simplifying the process

Describes a rapid method (10 days) to generate multivirus-specific CTLs using pepmixes and IL4/IL7. The method uses the G-Rex10 device for stimulation and expansion, allowing the production of large numbers (1x10^8) of virus-specific T cells from a small starting number of PBMCs with minimal manipulation

Details a simplified TIL production method using G-Rex. G-Rex10 is used for initial culture from tumor fragments/digests, and G-Rex100 for rapid expansion (REP). This method reduces media use by 3-4 times and requires fewer vessels and labor compared to traditional 24-well plates and bags

Describes generating CTL lines targeting multiple tumor-associated antigens (SSX2, MAGEA4, Survivin, etc.) for lymphoma. Uses optimized cytokine cocktails (IL-7, IL-12, IL-15, IL-6/IL-27) for activation. While not explicitly detailed in the excerpts, G-Rex is standard for this group's expansion protocols

Compares the WAVE to static gas-permeable bags for TIL and PBL expansion. While total cell numbers were comparable, the WAVE produced a higher percentage of CD4+ cells and a less activated phenotype. The study references G-Rex (Vera 2010) as an alternative static option

Reviews optimization of antigen-specific T cell manufacturing. Highlights the G-Rex device as a revolutionary tool that increases cell yields by 3-20 fold while reducing manipulation. G-Rex allows unrestricted media depth due to gas exchange from the bottom, linearly scalable from research to GMP production

Describes using the PiggyBac transposon system to modify EBV-CTLs to express HER2-CARs. The G-Rex device (G-Rex10 and G-Rex100) was used to expand the gene-modified CTLs, supporting long-term survival and stable transgene expression. G-Rex allowed efficient expansion of clinical-grade cells with minimal manipulation

Outlines GMP manufacturing of LMP1/LMP2-specific CTLs for EBV-associated lymphoma. Describes using G-Rex devices for the second stimulation and expansion phases to support high-density cell growth. G-Rex simplifies the process and reduces labor compared to traditional 24-well plates while maintaining cell quality

Evaluates G-Rex for expanding antigen-specific CTLs. G-Rex supports 3-20 fold higher cell output than 24-well plates by improving gas exchange and allowing deeper media. The device simplifies manufacturing by reducing interventions and supports high-density culture of CTLs and CAR-T cells without compromising function

Investigates culturing pancreatic islets on gas-permeable silicone rubber membranes. This method allows for higher surface densities (up to 4000 IE/cm²) and media depths compared to standard without hypoxic damage. G-Rex technology principles are tested here for islet survival and function

This study presents a protocol for large-scale expansion of NKG2C+ NK cells using PC-3 feeder cells. G-Rex 6M well plates were employed to achieve high expansion factors (median 358.5-fold) of functional NK cells for glioblastoma immunotherapy.