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Publications

Improved Homing to Bone Marrow, Spleen and Lung of Adoptively Infused NK Cells Expanded Ex Vivo with the Small Molecule Nicotinamide Using Feeder-Free Conditions

This study demonstrates that nicotinamide (NAM) enhances the homing and retention of NK cells in bone marrow, spleen, and lung. NK cells were expanded in G-Rex devices under feeder-free conditions with IL-2 or IL-15. The G-Rex platform supported large-scale expansion, yielding high-purity NK cells.
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Publications

Safety and clinical efficacy of rapidly-generated trivirus-directed T cells as treatment for adenovirus, EBV, and CMV infections after allogeneic hematopoietic stem cell transplant

This study reports a simplified method to generate T cells targeting EBV, CMV, and Adenovirus using nucleofected dendritic cells and G-Rex devices. The 10-day G-Rex protocol produced clinically relevant cell numbers. Infused cells were safe and effective against active viral infections in HSCT recipients.
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Publications

Randomized Selection Design Trial Evaluating CD8+-Enriched Versus Unselected Tumor-Infiltrating Lymphocytes for Adoptive Cell Therapy for Patients With Melanoma

Compares unselected young TILs vs CD8+-enriched TILs. Mentions that G-Rex were optimized for TIL therapy and introduced for clinical use. The study used (WAVE and G-Rex) for high-density T-cell expansion, facilitating rapid accrual
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Publications

Adoptive Transfer of Tumor-Infiltrating Lymphocytes in Patients with Metastatic Melanoma: Intent-to-Treat Analysis and Efficacy after Failure to Prior Immunotherapies

Clinical trial results for TIL therapy in melanoma. Mentions using 'gas-permeable bags or GRex ' for the Rapid Expansion Procedure (REP). Notes that G-Rex allow large-scale expansion to treatment levels (10^10 - 10^11 cells)
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Publications

Robust and cost effective expansion of human regulatory T cells highly functional in a xenograft model of graft-versus-host disease

Describes a protocol for expanding nTregs using G-Rex10 and G-Rex100 devices. The method yielded >10^9 Tregs in 21 days with high purity and suppressive function. G-Rex promoted robust expansion (over 600-fold) compared to 24-well plates (5-6 fold) and maintained telomere length
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Publications

SOP: D03.31.22 INITIATION OF EBV- OR ANTIGEN-SPECIFIC CYTOTOXIC T LYMPHOCYTES (CTL)

SOP for initiating CTLs. Describes initiating cells in '24-well plate or GRex40 '. Details seeding 1x10^7 cells in 5mL for G-Rex40 and culturing for 9 days for initial stimulation
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Publications

SOP: D03.32.21 EXPANSION OF EBV- OR ANTIGEN-SPECIFIC CYTOTOXIC T LYMPHOCYTES (CTL)

SOP for expanding CTLs. Detailed instructions for using G-Rex40 and G-Rex500. Describes transfer of cells to G-Rex, adding media and IL-2, feeding schedules (removing half media), and splitting cells if density is high
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Publications

SOP: D03.05.22 CHARACTERIZATION AND FREEZING OF CYTOTOXIC T-LYMPHOCYTES

SOP for freezing CTLs. Mentions harvesting CTLs from 'wells or GRex '. Provides a protocol for ensuring cell quality and viability during cryopreservation
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Publications

SOP: D03.14.23 ADENOVIRUS TRANSDUCTION OF B95-8+ LCL FOR CTL STIMULATION

SOP for transducing LCLs with adenovirus vectors. G-Rex is not explicitly referenced in this document, though it is part of a larger G-Rex workflow for CTL production
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Publications

SOP: D03.01.23 GENERATION OF EBV-TRANSFORMED CELL LINES (LYMPHOBLASTOID CELL LINES - LCL)

SOP for generating LCLs. Mentions expansion of LCLs in ' (G40 and G500)' (G-Rex) if large numbers are required. Describes seeding densities and media volumes for G-Rex40 (30 mL) and G-Rex500 (100-200 mL)
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Publications

Large-scale ex vivo expansion and characterization of natural killer cells for clinical applications

Describes a static culture protocol for expanding NK cells using K562-mb15-41BBL feeder cells in G-Rex devices. The G-Rex system yielded higher fold expansion (>100-fold) and total cell numbers compared to gas-permeable bags, with no feeding required during the 8-10 day culture period
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Publications

Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein–Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant

Details the GMP manufacturing process for trivirus-specific CTLs (CMV, EBV, Adv). The protocol allows for stimulation and expansion in either 24-well plates or G-Rex devices (specifically G-Rex40 and G-Rex500). G-Rex is noted for supporting high-density cell growth and simplifying the process
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