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This study demonstrates that CAR-redirected EBV-CTLs engineered to express IL-7Ra can be selectively expanded by IL-7 in the presence of Tregs. G-Rex were used to generate the expanded Tregs used in the suppression assays. This strategy enhances antitumor activity.

This study developed a protocol for generating canine CAR T cells targeting HER2. G-Rex culture devices were used to expand the activated canine T cells, achieving approximately 100-fold expansion in 8 days. The cells demonstrated antigen-specific cytotoxicity against canine osteosarcoma.

This study compared fresh activated NK cells vs. ex vivo expanded NK cells (Ex-NK) produced in G-Rex devices. Ex-NK cells showed distinct homing to bone marrow and higher cytokine dependence (IL-15) compared to fresh cells. The study highlights how manufacturing methods influence in vivo behavior.

This review discusses methods for clinical-grade NK cell expansion. It highlights the optimization of NK cell expansion in G-Rex using K562-mbIL15-41BBL feeder cells, achieving high yields (up to 5x10^8 cells per G-Rex100) with reduced handling compared to bags.

This study engineered EBV-specific T cells with a chimeric IL4/7 receptor to convert inhibitory IL4 signals into stimulatory IL7 signals. G-Rex were utilized to maintain the EBV-LCL feeder cells used for T cell stimulation. The modified cells showed enhanced antitumor activity in IL4-rich environments.

This study evaluates shipping human islets in high density using silicone rubber membrane (SRM) vessels (G-Rex). The method maintained islet viability and function comparable to low-density controls, allowing an entire clinical islet isolation to be shipped in a single vessel.

This dissertation analyzes human Treg subsets and their TCR repertoires. It demonstrates that expanding Tregs in G-Rex10 devices with rapamycin enhances expansion (at least 50-fold) compared to plates, while maintaining FoxP3 expression and suppressive function, facilitating clinical translation.

This is the published version of the study optimizing TIL propagation using K562 aAPCs. It confirms that the aAPC/G-Rex platform supports rapid expansion of TILs to clinical scales with an effector-memory phenotype, comparable to PBMC feeders. Note: This is a duplicate study of 2014_Forgetet_al_TIL.pdf.

This study defines optimized parameters for the G-Rex 'M' series using K562 cells. It demonstrates that 1L of medium with optimal seeding allows 100-fold expansion in 10 days in the G-Rex 100M. The platform showed linear scalability from 5 to 500 cm2 and validated glucose measurement for cell quantification.

This study reports the expansion of HSPCs using Tat-MYC and Tat-Bcl-2 fusion proteins. G-Rex 10 devices were used to expand CD34+ cells from cord blood, mobilized blood, and bone marrow. The expanded cells retained long-term repopulating activity and differentiated into multiple lineages in vivo.

This conference paper reports a method for large-scale generation of myeloid dendritic cells (DCs) from cord blood CD34+ cells using G-Rex10. The G-Rex system yielded over 1.5 billion DCs, significantly outperforming conventional culture plates, and produced DCs capable of inducing Th1 responses.

This research investigates the kinetics of tumor destruction by CAR T cells targeting MUC1 and PSCA. G-Rex 100M devices were used to expand the CAR-modified T cells. The study found that dual targeting improved efficacy but antigen intensity influenced killing; epigenetic modulation enhanced tumor susceptibility.