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Publications
Publications

Towards immunotherapy with redirected T cells in a large animal model: Ex vivo activation, expansion, and genetic modification of canine T cells

This study developed a protocol for generating canine CAR T cells targeting HER2. G-Rex culture devices were used to expand the activated canine T cells, achieving approximately 100-fold expansion in 8 days. The cells demonstrated antigen-specific cytotoxicity against canine osteosarcoma.
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Publications

Expansion and Homing of Adoptively Transferred Human NK Cells in Immunodeficient Mice Varies with Product Preparation and In Vivo Cytokine Administration: Implications for Clinical Therapy

This study compared fresh activated NK cells vs. ex vivo expanded NK cells (Ex-NK) produced in G-Rex devices. Ex-NK cells showed distinct homing to bone marrow and higher cytokine dependence (IL-15) compared to fresh cells. The study highlights how manufacturing methods influence in vivo behavior.
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Publications

Clinical Grade Purification and Expansion of Natural Killer Cells

This review discusses methods for clinical-grade NK cell expansion. It highlights the optimization of NK cell expansion in G-Rex using K562-mbIL15-41BBL feeder cells, achieving high yields (up to 5x10^8 cells per G-Rex100) with reduced handling compared to bags.
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Publications

Reversal of Tumor Immune Inhibition Using a Chimeric Cytokine Receptor

This study engineered EBV-specific T cells with a chimeric IL4/7 receptor to convert inhibitory IL4 signals into stimulatory IL7 signals. G-Rex were utilized to maintain the EBV-LCL feeder cells used for T cell stimulation. The modified cells showed enhanced antitumor activity in IL4-rich environments.
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Publications

Human Islet Viability and Function Is Maintained During High-density Shipment in Silicone Rubber Membrane Vessels

This study evaluates shipping human islets in high density using silicone rubber membrane (SRM) vessels (G-Rex). The method maintained islet viability and function comparable to low-density controls, allowing an entire clinical islet isolation to be shipped in a single vessel.
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Publications

Human natural regulatory T cells subsets: Functional characterization and T cell receptor repertoire analysis

This dissertation analyzes human Treg subsets and their TCR repertoires. It demonstrates that expanding Tregs in G-Rex10 devices with rapamycin enhances expansion (at least 50-fold) compared to plates, while maintaining FoxP3 expression and suppressive function, facilitating clinical translation.
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Publications

Activation and Propagation of Tumor-infiltrating Lymphocytes on Clinical-grade Designer Artificial Antigen-presenting Cells for Adoptive Immunotherapy of Melanoma

This is the published version of the study optimizing TIL propagation using K562 aAPCs. It confirms that the aAPC/G-Rex platform supports rapid expansion of TILs to clinical scales with an effector-memory phenotype, comparable to PBMC feeders. Note: This is a duplicate study of 2014_Forgetet_al_TIL.pdf.
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Publications

Optimizing the production of suspension cells using the G-Rex “M” series

This study defines optimized parameters for the G-Rex 'M' series using K562 cells. It demonstrates that 1L of medium with optimal seeding allows 100-fold expansion in 10 days in the G-Rex 100M. The platform showed linear scalability from 5 to 500 cm2 and validated glucose measurement for cell quantification.
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Publications

Expansion of Human and Murine Hematopoietic Stem and Progenitor Cells Ex Vivo without Genetic Modification Using MYC and Bcl-2 Fusion Proteins

This study reports the expansion of HSPCs using Tat-MYC and Tat-Bcl-2 fusion proteins. G-Rex 10 devices were used to expand CD34+ cells from cord blood, mobilized blood, and bone marrow. The expanded cells retained long-term repopulating activity and differentiated into multiple lineages in vivo.
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Publications

Large-scale myeloid dendritic cells generation from umbilical CD34+ cells using G-rex

This conference paper reports a method for large-scale generation of myeloid dendritic cells (DCs) from cord blood CD34+ cells using G-Rex10. The G-Rex system yielded over 1.5 billion DCs, significantly outperforming conventional culture plates, and produced DCs capable of inducing Th1 responses.
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Publications

Kinetics of Tumor Destruction by Chimeric Antigen Receptor-modified T Cells

This research investigates the kinetics of tumor destruction by CAR T cells targeting MUC1 and PSCA. G-Rex 100M devices were used to expand the CAR-modified T cells. The study found that dual targeting improved efficacy but antigen intensity influenced killing; epigenetic modulation enhanced tumor susceptibility.
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Publications

Antigen Presenting Cell-Mediated Expansion of Human Umbilical Cord Blood Yields Log-Scale Expansion of Natural Killer Cells with Anti-Myeloma Activity

This study utilized G-Rex to expand NK cells from cord blood using artificial antigen presenting cells (aAPCs). The protocol achieved a mean 2389-fold expansion of high-purity NK cells in 14 days. Expanded cells demonstrated significant in vitro and in vivo cytotoxicity against multiple myeloma.
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