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Publications

Adoptive transfer of ex vivo expanded SARS- CoV- 2- specific cytotoxic lymphocytes: A viable strategy for COVID- 19 immunosuppressed patients?

This study shows the feasibility of expanding SARS-CoV-2 VSTs from convalescent donors. CD14 negative fractions were seeded in a G-Rex system for a 21-day expansion protocol involving peptide-loaded DCs and PHA blasts, yielding specific T cell products.
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Publications

Innovative therapeutic strategy for B- cell malignancies that combines obinutuzumab and cytokine- induced killer cells

This study presents a therapeutic strategy combining CIK cells with the anti-CD20 antibody obinutuzumab. CIK cells were successfully expanded from patient PBMCs using G-Rex gas-permeable. The combination showed high cytotoxicity against B-cell malignancies in vitro and in vivo.
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Publications

High-dose TIL product with improved phenotype and functionality

This study presents an improved TIL production process. Using G-Rex vessels and TIL stimulators, production time was reduced to 3 weeks. G-Rex M 6-well plates were used for rapid expansion, yielding high doses of functional CD8+ T cells.
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Publications

Base-edited CAR T cells for combinational therapy against T cell malignancies

This paper describes base-edited, fratricide-resistant CAR-T cells targeting CD3 and CD7. Transduced cells were cultured and expanded in G-Rex 24 well, G-Rex 10, or G-Rex 100 chambers. The cells exhibited potent cytotoxicity against T-cell leukemias in vivo.
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Publications

Rapid GMP-Compliant Expansion of SARS-CoV-2–Specific T Cells From Convalescent Donors for Use as an Allogeneic Cell Therapy for COVID-19

This study established a method to expand SARS-CoV-2 specific T cells from convalescent donors. Using G-Rex culture vessels, including CS100M, the protocol achieved high yields of central memory T cells within 21 days for potential allogeneic therapy.
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Publications

Pilot study of BKV-specific T cells for immunotherapy of progressive multifocal leukoencephalopathy

This pilot study evaluated BKV-specific T cells (PyVST) for PML treatment. PyVSTs were generated from related donors and expanded in G-Rex tissue culture containers for 14 days. The therapy was safe, with some patients showing survival and viral load reduction.
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Publications

Tumor-infiltrating lymphocyte treatment for anti-PD-1 resistant metastatic lung cancer: a phase I trial

This Phase I trial assessed TIL therapy in metastatic NSCLC. Autologous TILs were expanded using a rapid expansion protocol in G-Rex 100 MCS. The treatment demonstrated manageable toxicity and induced durable complete responses in some patients.
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Publications

Engineering CAR- NK cells to secrete IL- 15 sustains their anti- AML functionality but is associated with systemic toxicities

This study engineered CAR-NK cells to secrete IL-15, enhancing their anti-AML activity and persistence. G-Rex plates were utilized for a serial stimulation assay to evaluate NK cell proliferation and cytotoxicity over a 10-day period.
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Publications

Cytometric analysis of T cell phenotype using cytokine profiling for improved manufacturing of an EBV- specific T cell therapy

This study improved EBV-specific T cell manufacturing using GMP reagents and G-Rex. G-Rex100 yielded higher CD8+ T cell percentages and expansion compared to standard flasks. Detailed cytokine profiling characterized the product phenotype.
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Publications

Acute exercise increases immune responses to SARS CoV-2 in a previously infected man

This study found that exercise mobilizes SARS-CoV-2 specific T-cells and antibodies. To assess T-cell function, SARS-CoV-2 virus-specific T-cells were expanded ex vivo from PBMCs using gas-permeable G-Rex devices for 7 days.
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Publications

Manufacturing NKG2D CAR-T cells with piggyBac transposon vectors and K562 artificial antigen-presenting cells

This paper details a manufacturing process for NKG2D CAR-T cells using piggyBac vectors and K562 aAPCs. It demonstrates robust expansion and cytotoxicity. While the specific protocol uses T75 flasks, it cites previous work utilizing G-Rex vessels for large-scale T cell expansion.
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Publications

Innovative Development of Closed CAR-T Platform

This poster describes a modular, closed CAR-T manufacturing platform. It details expansion strategies using G-Rex 100MCS for low doses and a two-step process with G-Rex 500MCS for high doses, consistently achieving high cell viability and yields.
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