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Publications
Publications

Freezing Medium Containing 5% DMSO Enhances the Cell Viability and Recovery Rate After Cryopreservation of Regulatory T Cell Products ex vivo and in vivo

This research optimizes cryopreservation strategies for regulatory T cell (Treg) products. G-Rex were used for the culture and expansion of Treg cells and for transporting fresh nTreg products. Results show that a freezing medium with 5% DMSO significantly improves cell viability and recovery.
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Publications

Enzymatically produced piggyBac transposon vectors for efficient non-viral manufacturing of CD19-specific CAR T cells

The study presents a manufacturing platform for CD19-specific CAR T cells using enzyme-produced piggyBac transposon DNA and mRNA transposase. G-Rex10 were utilized for large-scale expansion, yielding up to 10^8 CAR-T cells per electroporation. The method allows precise control of vector copy number.
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Publications

Differentiation of natural killer cells from induced pluripotent stem cells under defined, serum- and feeder-free conditions

This study evaluates differentiating NK cells from iPSCs under chemically defined, serum-free conditions without feeder layers. G-Rex plates were used to expand hiPSC01-derived NK cells and peripheral blood NK cells for 2 weeks, achieving high fold expansion. The method yields functional, cytotoxic NK cells.
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Publications

p16INK4a Regulates Cellular Senescence in PD-1-Expressing Human T Cells

This paper investigates the role of p16INK4a in T cell senescence. G-Rex and G-Rex 24-well plates were used to culture T cells for polyclonal activation and antigen-specific line generation, facilitating the study of long-term dysfunction.
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Publications

Immune inactivation of anti-simian immunodeficiency virus chimeric antigen receptor T cells in rhesus macaques

This study evaluates anti-SIV CAR-T cells in rhesus macaques. Host antibodies inhibited CAR-T expansion in vivo. G-Rex were used during manufacturing to pool and expand CD4+ and CD8+ CAR-T cells prior to infusion into the animals.
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Publications

Mutated GM-CSF-based CAR-T cells targeting CD116/CD131 complexes exhibit enhanced anti-tumor effects against acute myeloid leukaemia

This study developed mutated GM-CSF-based CAR-T cells targeting CD116/CD131 for AML. The CAR-T cells showed enhanced anti-tumor effects. G-Rex usage was not explicitly mentioned in the provided text.
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Publications

NK cell receptor and ligand composition influences the clearance of SARS-CoV-2

This study correlates NK cell receptor repertoires with SARS-CoV-2 clearance. G-Rex 24-well culture plates were used to expand primary NK cells with K562 feeder cells to obtain sufficient cell numbers for functional killing assays.
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Publications

Novel CS1 CAR-T Cells and Bispecific CS1-BCMA CAR-T Cells Effectively Target Multiple Myeloma

This study develops CS1 and bispecific CS1-BCMA CAR-T cells. Bispecific CAR-T cells were effectively expanded over 180-fold in a 1 L G-Rex system, maintaining a central memory phenotype and demonstrating high specific cytotoxicity against multiple myeloma cells.
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Publications

Adoptive transfer of ex vivo expanded SARS- CoV- 2- specific cytotoxic lymphocytes: A viable strategy for COVID- 19 immunosuppressed patients?

This study shows the feasibility of expanding SARS-CoV-2 VSTs from convalescent donors. CD14 negative fractions were seeded in a G-Rex system for a 21-day expansion protocol involving peptide-loaded DCs and PHA blasts, yielding specific T cell products.
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Publications

Innovative therapeutic strategy for B- cell malignancies that combines obinutuzumab and cytokine- induced killer cells

This study presents a therapeutic strategy combining CIK cells with the anti-CD20 antibody obinutuzumab. CIK cells were successfully expanded from patient PBMCs using G-Rex gas-permeable. The combination showed high cytotoxicity against B-cell malignancies in vitro and in vivo.
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Publications

High-dose TIL product with improved phenotype and functionality

This study presents an improved TIL production process. Using G-Rex vessels and TIL stimulators, production time was reduced to 3 weeks. G-Rex M 6-well plates were used for rapid expansion, yielding high doses of functional CD8+ T cells.
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Publications

Base-edited CAR T cells for combinational therapy against T cell malignancies

This paper describes base-edited, fratricide-resistant CAR-T cells targeting CD3 and CD7. Transduced cells were cultured and expanded in G-Rex 24 well, G-Rex 10, or G-Rex 100 chambers. The cells exhibited potent cytotoxicity against T-cell leukemias in vivo.
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