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Publications
Publications

Adaptive single- KIR+NKG2C+ NK cells expanded from select superdonors show potent missing- self reactivity and efficiently control HLA- mismatched acute myeloid leukemia

This study describes the ADAPT-NK cell product derived from superdonors. The expansion protocol involved co-culturing CD3/CD19-depleted PBMCs with feeder cells in G-Rex 24 plates for 11 days to generate high numbers of adaptive NK cells.
Publications

Salt- inducible kinase 3 protects tumor cells from cytotoxic T- cell attack by promoting TNF- induced NF- κB activation

The study identifies SIK3 as a gene protecting tumor cells from T-cell cytotoxicity. In the methods, TILs were rapidly expanded using a modified Rosenberg protocol involving culture in G-Rex 100 with feeder cells and IL-2.
Publications

Improving multiTAA-Specific T Cell Phenotype and Potency Through Process Evolution

The study outlines improvements to the manufacturing of MT-401 (multiTAA-specific T cells). The new process using G-Rex 10M and scaling to G-Rex 500M reduced duration to 9 days and improved T cell purity and memory phenotype.
Publications

in vitro 3D Tumor Models using the Go-Rex Platform

This poster introduces the Go-Rex device, built on G-Rex technology, for long-term 3D tumor modeling. It allows assessment of T-cell migration, infiltration, and killing over extended periods compared to traditional assays.
Publications

Combined IL- 2, agonistic CD3 and 4- 1BB stimulation preserve clonotype hierarchy in propagated non- small cell lung cancer tumor- infiltrating lymphocytes

The study compares TIL expansion methods. TIL 3.0 (IL-2 + anti-CD3 + anti-4-1BB) in G-Rex expanded more TILs with better CD8+ enrichment and TCR diversity than standard IL-2. The REP phase also used GREX-10M.
Publications

High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails

Describes a non-viral CRISPR/Cas9 method using ssCTS templates for high-yield CAR-T production. A GMP-compatible process was developed where cells were expanded in G-Rex 100M gas-permeable culture vessels for 7-10 days.
Publications

Validation of CD98hc as a Therapeutic Target for a Combination of Radiation and Immunotherapies in Head and Neck Squamous Cell Carcinoma

This paper explores combining radiotherapy with CD98hc-targeted UniCAR T cells for HNSCC. The combo showed synergistic anti-tumor effects. Genetically modified T cells were seeded in 24-well G-Rex plates and expanded for 4–6 days.
Publications

Generation and proof- of- concept for allogeneic CD123 CAR- Delta One T (DOT) cells in acute myeloid leukemia

The study generates and validates allogeneic CD123 CAR-Delta One T (DOT) cells for AML. These cells showed potent cytotoxicity against AML lines and primary samples. DOT cells were generated from alpha-beta depleted PBMCs cultured in either 96-well plates or the G-REX platform.
Publications

T-Cells Expressing a Highly Potent PRAME-Specific T-Cell Receptor in Combination with a Chimeric PD1-41BB Co-Stimulatory Receptor Show a Favorable Preclinical Safety Profile and Strong Anti-Tumor Reactivity

This study evaluates PRAME-specific TCR-Ts co-expressing a chimeric PD1-41BB receptor. These cells showed enhanced cytotoxicity against PRAME+ tumors in vitro and in vivo. G-Rex were used for the 10-day expansion phase of T-cells after transduction.
Publications

CAR/CXCR5-T cell immunotherapy is safe and potentially efficacious in promoting sustained remission of SIV infection

This study evaluates CAR/CXCR5-T cells for treating SIV infection in rhesus macaques. The cells homed to lymphoid follicles and reduced viral loads. Transduced T cells were expanded in G-Rex 500M-CS containers and 6-well plates during manufacturing.
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Publications

SARS-CoV-2-specific T cells generated for adoptive immunotherapy are capable of recognizing multiple SARS-CoV-2 variants

SARS-CoV-2-specific T cells were generated from convalescent donors for adoptive immunotherapy. These cells recognized multiple viral variants and provided broad HLA coverage. G-Rex culture vessels were used to culture and expand the peptide-stimulated PBMCs.
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Publications

HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy

The HydrAd platform uses helper-dependent adenovirus (HDAd) vectors to express multiple immunomodulatory transgenes for cancer therapy. It was evaluated with T cells and NK cells. NK cells used in the study were expanded in G-Rex cell culture devices.
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