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Publications
Publications

Context-specific synthetic T cell promoters from assembled transcriptional elements

The study creates synthetic promoters for CAR-T cells. T cells nucleofected with piggyBac transposase were transferred to G-REX culture vessels for 21 days of expansion.
Publications

Preparation of cryopreserved chimeric antigen receptor T cells for the locoreogional delivery to the neural axis

Describes a thaw/wash method for CAR-T cells for intracranial delivery. B7-H3-CAR T cells were generated and expanded in G-Rex culture devices using X-VIVO 15 media with cytokines.
Publications

Vγ9Vδ2 T cells expressing a BCMA—Specific chimeric antigen receptor inhibit multiple myeloma xenograft growth

This study generates BCMA-CAR Vg9Vd2 T cells via mRNA electroporation. Vg9Vd2 T cells were expanded using Zometa and co-cultured with aAPCs. Previous work by the group used G-Rex vessels for large-scale expansion.
Publications

Humanized CD19-directed CAR-T Cell Therapy in Pediatric Relapsed/Refractory Acute Lymphoblastic Leukemia With CNSL or Neurological Comorbidity

This clinical study evaluates humanized CD19 CAR-T cells in pediatric R/R ALL with CNS issues. Transduced T cells were cultured, amplified, and harvested over 5–7 days using the G-Rex system.
Publications

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Development of BAFF ligand-based CAR-T cells targeting BAFF-R, BCMA, and TACI. Cells were modified using the TcBuster transposon system and maintained in G-Rex culture vessels.
Publications

Chimeric antigen receptor structure and manufacturing of clinical grade CAR engineered cells using different bioreactors

This review discusses CAR structure and automated manufacturing platforms. It highlights the G-Rex (M series) and GatheRex pump as a practical, cost-effective, and scalable static culture option for CAR-T expansion.
Publications

Feasibility and preclinical efficacy of CD7-unedited CD7 CAR T cells for T cell malignancies

The study demonstrates manufacturing of CD7 CAR T cells using ibrutinib/dasatinib to prevent fratricide. cGMP manufacturing involved transduction and expansion in G-Rex culture devices for 3 additional days.
Publications

Improving cytokine-induced killer cell expansion using a gas-permeable culture method for clinical-scale production

This paper compares CIK cell expansion in G-Rex devices vs standard plates. G-Rex 24-well and 6-well plates were used. G-Rex resulted in significantly higher expansion and IFN-g secretion, supporting clinical-scale production.
Publications

Adaptive single- KIR+NKG2C+ NK cells expanded from select superdonors show potent missing- self reactivity and efficiently control HLA- mismatched acute myeloid leukemia

This study describes the ADAPT-NK cell product derived from superdonors. The expansion protocol involved co-culturing CD3/CD19-depleted PBMCs with feeder cells in G-Rex 24 plates for 11 days to generate high numbers of adaptive NK cells.
Publications

Salt- inducible kinase 3 protects tumor cells from cytotoxic T- cell attack by promoting TNF- induced NF- κB activation

The study identifies SIK3 as a gene protecting tumor cells from T-cell cytotoxicity. In the methods, TILs were rapidly expanded using a modified Rosenberg protocol involving culture in G-Rex 100 with feeder cells and IL-2.
Publications

Improving multiTAA-Specific T Cell Phenotype and Potency Through Process Evolution

The study outlines improvements to the manufacturing of MT-401 (multiTAA-specific T cells). The new process using G-Rex 10M and scaling to G-Rex 500M reduced duration to 9 days and improved T cell purity and memory phenotype.
Publications

in vitro 3D Tumor Models using the Go-Rex Platform

This poster introduces the Go-Rex device, built on G-Rex technology, for long-term 3D tumor modeling. It allows assessment of T-cell migration, infiltration, and killing over extended periods compared to traditional assays.

ScaleReady is a joint venture between Wilson Wolf Manufacturing and Bio-Techne.