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Publications

Large Scale Ex Vivo Expansion of γδ T cells Using Artificial Antigen-presenting Cells

This study demonstrates a GMP-compliant protocol for expanding donor-derived γδ T cells using zoledronic acid, IL-2, and engineered aAPCs. The method achieved significant expansion of Vδ1 and Vδ2 subsets. PBMCs were seeded into G-Rex 100 for the initial enrichment and expansion phases.
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Publications

Homology‑independent targeted insertion (HITI) enables guided CAR knock‑in and efficient clinical scale CAR‑T cell manufacturing

Describes a non-viral CRISPR/Cas9 HITI method for CAR knock-in. Primary T cells were cultivated in G-Rex 24-well/6-well plates or G-Rex 100M vessels.
Publications

Scalable GMP-compliant gene correction of CD4+ T cells with IDLV template functionally validated in vitro and in vivo

Developed a GMP process for gene correction of CD4+ T cells. Medium-scale experiments used G-Rex 6-6M, and large-scale used G-Rex 100CS or 500CS for cell expansion.
Publications

Preclinical Evidence of an Allogeneic Dual CD20xCD22 CAR to Target a Broad Spectrum of Patients with B-cell Malignancies

This study presents preclinical data for allogeneic CD20xCD22 CAR T cells. Cells were cultured in 10 cm G-Rex or 6-well G-Rex devices during the manufacturing process.
Publications

Context-specific synthetic T cell promoters from assembled transcriptional elements

The study creates synthetic promoters for CAR-T cells. T cells nucleofected with piggyBac transposase were transferred to G-REX culture vessels for 21 days of expansion.
Publications

Preparation of cryopreserved chimeric antigen receptor T cells for the locoreogional delivery to the neural axis

Describes a thaw/wash method for CAR-T cells for intracranial delivery. B7-H3-CAR T cells were generated and expanded in G-Rex culture devices using X-VIVO 15 media with cytokines.
Publications

Vγ9Vδ2 T cells expressing a BCMA—Specific chimeric antigen receptor inhibit multiple myeloma xenograft growth

This study generates BCMA-CAR Vg9Vd2 T cells via mRNA electroporation. Vg9Vd2 T cells were expanded using Zometa and co-cultured with aAPCs. Previous work by the group used G-Rex vessels for large-scale expansion.
Publications

Humanized CD19-directed CAR-T Cell Therapy in Pediatric Relapsed/Refractory Acute Lymphoblastic Leukemia With CNSL or Neurological Comorbidity

This clinical study evaluates humanized CD19 CAR-T cells in pediatric R/R ALL with CNS issues. Transduced T cells were cultured, amplified, and harvested over 5–7 days using the G-Rex system.
Publications

A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers

Development of BAFF ligand-based CAR-T cells targeting BAFF-R, BCMA, and TACI. Cells were modified using the TcBuster transposon system and maintained in G-Rex culture vessels.
Publications

Chimeric antigen receptor structure and manufacturing of clinical grade CAR engineered cells using different bioreactors

This review discusses CAR structure and automated manufacturing platforms. It highlights the G-Rex (M series) and GatheRex pump as a practical, cost-effective, and scalable static culture option for CAR-T expansion.
Publications

Feasibility and preclinical efficacy of CD7-unedited CD7 CAR T cells for T cell malignancies

The study demonstrates manufacturing of CD7 CAR T cells using ibrutinib/dasatinib to prevent fratricide. cGMP manufacturing involved transduction and expansion in G-Rex culture devices for 3 additional days.
Publications

Improving cytokine-induced killer cell expansion using a gas-permeable culture method for clinical-scale production

This paper compares CIK cell expansion in G-Rex devices vs standard plates. G-Rex 24-well and 6-well plates were used. G-Rex resulted in significantly higher expansion and IFN-g secretion, supporting clinical-scale production.

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