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Publications
Publications

Automated manufacturing of cell therapies

This review analyzes automated cell therapy manufacturing systems. It highlights the G-Rex as a key technology for simplified static expansion compared to flasks. It notes that the Multiply Labs robotic cluster integrates G-Rex 100M for automated, parallel T cell expansion.
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Publications

Tuning TCR complex recruitment to the T cell antigen coupler (TAC) enhances TAC-T cell function

This study demonstrates that tuning the affinity of the TAC receptor's TCR-binding domain improves in vivo anti-tumor potency. CLDN18.2-TAC T cells were engineered and expanded using the G-Rex system. Lower affinity UCHT1 variants led to superior functional outcomes in solid tumor models.
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Publications

Protocol for isolation and expansion of natural killer cells from human peripheral blood scalable for clinical applications

This protocol describes the isolation and feeder-cell-based expansion of NK cells. To demonstrate clinical scalability, the method was tested on GMP-grade G-Rex 100M platforms, achieving clinically relevant numbers of highly pure and functional NK cells suitable for adoptive therapy.
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Publications

RESET: A TCR-coupled antigen receptor with superior targeting sensitivity and reversible drug-regulated anti-tumor activity

This study introduces RESET, a two-component receptor system engaging the endogenous TCR with drug-regulated control. RESET-T cells exhibited superior sensitivity and potency compared to CARs. G-Rex vessels were used for the culture and expansion of the engineered T cells during the study.
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Publications

Optimizing the procedure for manufacturing clinical-grade genetically manipulated natural killer cells for adoptive immunotherapy

This study establishes an optimized platform for expanding and genetically modifying NK cells. Using KBM581 medium with serum replacement in 24-well G-Rex plates, the authors achieved over 5000-fold expansion in 14 days. The protocol supports efficient viral transduction and gene editing of NK cells.
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Publications

Enhancing CAR T-cell therapy manufacturing efficiency through semi-automated bioprocessing

This study demonstrates a semi-automated workflow for non-viral CAR T-cell production using digitally connected instruments. T cells were isolated, activated, and then cultured in G-Rex 100 and G-Rex 100M. The process yielded high-quality CAR T cells with reduced manual touchpoints and variability.
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Publications

Anti-CD137 agonist antibody– independent and clinically feasible preparation of tumor-infiltrating lymphocytes from soft tissue sarcoma and osteosarcoma

The authors developed a GMP-feasible protocol for expanding TILs from sarcoma without anti-CD137 agonist, using CD3/CD28 beads instead. The rapid expansion phase (REP) utilized G-Rex 10M, which achieved a 2- to 3-fold higher expansion compared to T-75 flasks, boosting clinical feasibility.
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Publications

Clinical manufacture of CRISPR/ Cas9-based cytokine-induced SH2 protein knock-out tumor-infiltrating lymphocytes for gastrointestinal cancers

This paper details the GMP manufacturing of CISH KO TILs for GI cancers. Tumor fragments were outgrown in 24-well G-Rex plates, and selected TILs were expanded in 100 cm² G-Rex vessels. An automated liquid handling system was developed to perform media exchanges directly in the G-Rex 24 plates.
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Publications

Good manufacturing practice production of an off-the-shelf CD34+ progenitor-derived NK cell product with preserved anti-tumor functionality post-infusion in NOD/SCID/IL2Rgnull mice

This study presents a GMP-compliant process for manufacturing off-the-shelf NK cells from CD34+ HSPCs. The protocol was adapted to G-Rex 10M and 100M to facilitate scaling and reduce handling. The resulting NK cells showed high recovery, viability, and potent anti-tumor activity in vivo and in vitro.
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Publications

Hypoimmune FK binding protein 12-knockout CD19 CAR T cells achieve deep tissue B-cell depletion and control Nalm6 tumors in immunosuppressed humanized mice

This paper describes the engineering of allogeneic CD19 CAR T cells with FKBP12 knockout to resist mTOR inhibitors like tacrolimus and rapamycin. The study utilized 24-well G-Rex plates for the recovery and expansion of nucleofected T cells. These cells demonstrated deep B-cell depletion in immunosuppressed mice.
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Publications

Clinical grade expansion protocol for the manufacture of thymus-derived Treg cells for clinical application

This study establishes a GMP-compliant protocol for expanding thymus-derived regulatory T cells (Thy-Tregs). By optimizing CD8 depletion, CD25 enrichment, and cytokine stimulation, the authors achieved high purity. G-Rex (G-Rex 10 and 100) were successfully used to scale up production for clinical trials.
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Publications

Cryopreserved G-CSF-Mobilized Apheresis Units in ATMP Manufacturing

This poster explores using cryopreserved G-CSF-mobilized apheresis units for manufacturing CIK and CAR-CIK cells. It compares manual versus automated washing and expansion methods. The use of G-Rex allowed for higher cell expansion in significantly less time (9-12 days) compared to standard.
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