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The study presents a 24-hour CAR-T manufacturing workflow. T cell cultures were maintained in G-Rex vessels during isolation and transduction, and control 7-day expansions were also performed in G-Rex vessels.

Tmod cells maintain robust potency and selectivity. Transduced cells were cultured in G-Rex 24 well plates, and T cells for in vivo studies were cultured in G-Rex 6 well plates.

The study describes the preclinical evaluation of TAC01-CLDN18.2 T cells. These cells were manufactured using purified CD4/CD8 T cells in a 9-day process utilizing either the G-Rex system from Wilson Wolf or the Cocoon platform.

This study explores combining CAR T cells with 177Lu-DOTATATE targeted radionuclide therapy. CAR T cells were transduced and subsequently expanded in G-Rex 6M well plates.

PF-07260437 demonstrates potent antitumor efficacy in breast cancer models. For adoptive T cell transfer studies, human T cells were expanded in a G-Rex cell culture device.

The authors developed a scalable process for expanding NK and CAR-NK cells using G-Rex 100M closed-system. The study showed that cells expanded in G-Rex 100M retained similar phenotypes and cytotoxicity to those grown in G-Rex 6-well plates.

This paper reports a method leveraging HMEJ for Cas9-induced targeted integration of large DNA payloads. In the lentiviral transduction comparison arm, T cells were activated and transferred to a G-Rex 24-well plate for expansion.

This study demonstrates that S309-CAR-NK cells can bind to SARS-CoV-2 variants and reduce viral loads in humanized ACE2-NSG mice. Primary NK cell expansion and CAR-NK cell maintenance were performed using G-REX wells.

This study introduces the YT-rCTL/KFRET system for rapid TCR functional profiling. YT-Indy based effector cell lines were subcultured in 24-well G-Rex plates. The platform allows for the recombinant expression of TCRs and antigens to assess cytotoxic responses via a granzyme-activatable reporter.

This study compares CAR-T expansion in Prodigy, Xuri, G-Rex, and bags. G-Rex (500M) cultures yielded effector memory-enriched cells. Hypoxic culture in G-Rex increased the proportion of naïve/stem central memory-like cells, suggesting oxygenation is a key factor in T cell differentiation during manufacturing.

This study demonstrates that proteasome inhibitors sensitize AML cells to NK cell killing. NK cells were activated by co-culture with irradiated feeder cells in G-Rex 10 vessels. The combination of bortezomib pre-treatment and CAR-NK infusion significantly delayed tumor growth and prolonged survival in xenograft models.

This study identifies placental circulating T cells as a superior source for allogeneic CAR-T therapy. T cells were activated and expanded in G-Rex plates. The resulting CAR-T cells retained stemness markers, exhibited a favorable cytokine profile, and demonstrated durable in vivo efficacy against lymphoma.