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Publications
Publications

IL-2-armored peptide-major histocompatibility class I bispecific antibodies redirect antiviral effector memory CD8+ T cells to induce potent anti-cancer cytotoxic activity with limited cytokine release

This study presents IL-2-armored GPS molecules that selectively expand and redirect CMV-specific CD8+ T cells. Expansion assays were conducted in 6-well and 24-well G-REX plates. The approach induced potent cytotoxicity against cancer cells with a reduced cytokine release profile compared to conventional TCEs.
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Publications

Perspectives for the Use of Umbilical Cord Blood in Transplantation and Beyond: Initiatives for an Advanced and Sustainable Public Banking Program in Greece

This report describes the operation of Greek public UCB banks. It highlights initiatives to reuse non-transplantable units for cell therapy. Specifically, it details the expansion of immunomagnetically selected CD34+ cells in G-Rex to differentiate into dendritic cells for generating leukemia-specific T cells.
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Publications

Generating universal anti-CD19 CAR T cells with a defined memory phenotype by CRISPR/ Cas9 editing and safety evaluation of the transcriptome

This study presents a method to generate universal CAR-T cells with a defined memory phenotype by editing TRAC and B2M genes. Cells were cultured in G-Rex 24-well plates. The safety of the editing was confirmed via CRISPRroots analysis, and the cells showed effective anti-tumor activity in vitro.
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Publications

Optimisation of a primary human CAR-NK cell manufacturing pipeline

This study optimizes CAR-NK manufacturing using primary NK cells. Expansion was performed in 48-well G-Rex plates using irradiated feeder cells. Adult PB-NK cells showed superior yield and cytotoxicity. The protocol achieved high transduction efficiency and scalable expansion suitable for clinical use.
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Publications

Therapeutic Effects of Aβ-Specific Regulatory T Cells in Alzheimer’s Disease: A Study in 5xFAD Mice

This study investigates the therapeutic effects of Aβ-specific Tregs in Alzheimer's disease mice. Purified CD4+CD25+ Tregs were expanded in G-Rex 24-well plates with CD3/28 MACSiBead particles and IL-2. Adoptive transfer improved cognitive function and reduced Aβ and pTAU accumulation in the hippocampus.
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Publications

Geometric parameters that affect the behavior of logic-gated CAR T cells

This study examines Tmod blocker function targeting non-HLA antigens, finding that blocker efficacy depends on antigen height. Primary human T cells were transduced with Tmod constructs and grown in G-Rex plates. The findings established geometric design principles to optimize Tmod selectivity and sensitivity.
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Publications

NOT gated T cells that selectively target EGFR and other widely expressed tumor antigens

This study describes Tmod cell therapy using a NOT gate to target widely expressed antigens like EGFR while sparing normal cells via an HLA-A*02 blocker. Primary T cells were transduced with CAR or Tmod lentivirus and cultured in G-Rex plates (G-Rex 10 for in vivo studies) to generate effector cells.
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Publications

Stem cell memory EBV- specific T cells control EBV tumor growth and persist in vivo

This study developed a protocol to expand EBV-specific TSCM-enriched T cells using IL-4, IL-7, and TWS-119. PBMCs were cultured in G-Rex 24-well or 6-well plates. The resulting cells demonstrated superior tumor infiltration, in vivo proliferation, and persistence compared to conventional protocols.
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Publications

PM21-particle stimulation augmented with cytokines enhances NK cell expansion and confers memory-like characteristics with enhanced survival

This study combined PM21-particles with IL-12/15/18 (CAP) to expand NK cells. CAP-NK cells showed superior expansion in G-Rex 24, G-Rex 6M, and G-Rex 100M. The resulting cells exhibited memory-like properties, enhanced metabolic fitness, and improved in vivo persistence compared to PM21 alone.
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Publications

IL2 Targeted to CD8+ T Cells Promotes Robust Effector T-cell Responses and Potent Antitumor Immunity

This study describes AB248, a CD8 cis-targeted IL2 that selectively activates CD8+ T cells. B7H3 CAR-T cells were generated by stimulating T cells and transducing them in a 24-well G-Rex plate. The combination of CAR-T and CD8-targeted IL2 resulted in potent antitumor activity and complete tumor rejection in mice.
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Publications

Good manufacturing practice-grade generation of CD19 and CD123-specific CAR-T cells using piggyBac transposon and allogeneic feeder cells in patients diagnosed with B-cell non-Hodgkin lymphoma and acute myeloid leukemia

This study presents a GMP protocol for non-viral CAR-T production using piggyBac transposons. Expansion was performed in G-Rex 24 plates (low scale) and G-Rex 10/100 bottles (large scale) with irradiated allogeneic feeders. The feeder improved yield in lymphopenic patients without affecting VCN or phenotype.
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Publications

Toll-like receptor 4 signaling activation domains promote CAR T cell function against solid tumors

This study investigated incorporating TLR4 signaling domains into CD19 CARs to improve efficacy against solid tumors. Primary T cells were transduced with CAR lentiviral vectors and expanded for 7-10 days in G-Rex six-well plates. The modified CAR-Ts exhibited enhanced cytotoxicity and cytokine secretion.
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