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Publications

Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances

The study reports the optimized GMP-grade manufacturing of TranspoCART19 cells. Following electroporation, cells were expanded for 11 days in G-Rex 100M devices, achieving high expansion and functionality.
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Publications

Cost-effective strategies for CAR-T cell therapy manufacturing

This review discusses cost-effective CAR-T manufacturing. It highlights G-Rex technology as a main point-of-care (POC) manufacturing platform that enables high cell density production at lower costs compared to fully automated systems.
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Publications

Accelerating CAR T cell manufacturing with an automated next-day process

The study presents a 24-hour CAR-T manufacturing workflow. T cell cultures were maintained in G-Rex vessels during isolation and transduction, and control 7-day expansions were also performed in G-Rex vessels.
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Publications

Detailed analysis of the effects of a NOT gate on activation and growth of T cells

Tmod cells maintain robust potency and selectivity. Transduced cells were cultured in G-Rex 24 well plates, and T cells for in vivo studies were cultured in G-Rex 6 well plates.
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Publications

Preclinical Development of T Cells Engineered to Express a T-Cell Antigen Coupler Targeting Claudin 18.2–Positive Solid Tumors

The study describes the preclinical evaluation of TAC01-CLDN18.2 T cells. These cells were manufactured using purified CD4/CD8 T cells in a 9-day process utilizing either the G-Rex system from Wilson Wolf or the Cocoon platform.
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Publications

Low-dose targeted radionuclide therapy synergizes with CAR T cells and enhances tumor response

This study explores combining CAR T cells with 177Lu-DOTATATE targeted radionuclide therapy. CAR T cells were transduced and subsequently expanded in G-Rex 6M well plates.
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Publications

A Novel B7-H4xCD3 Bispecific T Cell Engager (PF-07260437) Synergizes with Breast Cancer Standard of Care and Immune-Checkpoint Therapies

PF-07260437 demonstrates potent antitumor efficacy in breast cancer models. For adoptive T cell transfer studies, human T cells were expanded in a G-Rex cell culture device.
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Publications

Scalable process development of NK and CAR-NK expansion in a closed bioreactor

The authors developed a scalable process for expanding NK and CAR-NK cells using G-Rex 100M closed-system. The study showed that cells expanded in G-Rex 100M retained similar phenotypes and cytotoxicity to those grown in G-Rex 6-well plates.
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Publications

Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

This paper reports a method leveraging HMEJ for Cas9-induced targeted integration of large DNA payloads. In the lentiviral transduction comparison arm, T cells were activated and transferred to a G-Rex 24-well plate for expansion.
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Publications

S309-CAR-NK cells bind the Omicron variants in vitro and reduce SARS-CoV-2 viral loads in humanized ACE2-NSG mice

This study demonstrates that S309-CAR-NK cells can bind to SARS-CoV-2 variants and reduce viral loads in humanized ACE2-NSG mice. Primary NK cell expansion and CAR-NK cell maintenance were performed using G-REX wells.
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Publications

A synthetic cytotoxic T cell platform for rapidly prototyping TCR function

This study introduces the YT-rCTL/KFRET system for rapid TCR functional profiling. YT-Indy based effector cell lines were subcultured in 24-well G-Rex plates. The platform allows for the recombinant expression of TCRs and antigens to assess cytotoxic responses via a granzyme-activatable reporter.
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Publications

CAR-T cell expansion platforms yield distinct T cell differentiation states

This study compares CAR-T expansion in Prodigy, Xuri, G-Rex, and bags. G-Rex (500M) cultures yielded effector memory-enriched cells. Hypoxic culture in G-Rex increased the proportion of naïve/stem central memory-like cells, suggesting oxygenation is a key factor in T cell differentiation during manufacturing.

ScaleReady is a joint venture between Wilson Wolf Manufacturing and Bio-Techne.