Liquid Cytokines
Ready-to-use formulations – no reconstitution!
Simplify Your T-Cell Culture
Streamline your cell culture process with liquid-frozen formulations of animal-free RUO and GMP IL-2, IL-7, and IL-15. These ready-to-use cytokines eliminate reconstitution errors while providing the same potency and consistency as our lyophilized protein. Simply thaw and add to your cell culture!
Animal-Free RUO and GMP
ScaleReady utilizes Bio-Techne proteins produced in the only publicly known state-of-the-art certified animal-free facility, meaning you’re working with truly animal-free devoid of animal contaminants and mammalian pathogens.
- Streamlined Process: Save time and materials – no reconstitution
- Increased Reproducibility: Eliminate errors with reconstitution steps
- Equivalent Bioactivity: Easily substitute for lyophilized proteins
- Stability*: Rely on full bioactivity for at least six months as supplied.
Liquid Animal Free RUO and GMP Proteins- TABLE
| GMP IL-2 | Lyophilized | Liquid |
| Formulation | Lyophilized from a 0.2 µm filtered solution in sodium acetate with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in sodium acetate |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-002-GMP | BT-002-GMP/LQ |
| Pack Size | 50 µg, 1 mg | 50 µg, 1 mg |
| GMP IL-2 | Lyophilized | Liquid |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in PBS |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-007-GMP | BT-007-GMP/LQ |
| Pack Size | 25 µg, 1 mg | 25 µg |
| GMP IL-2 | Lyophilized | Liquid |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose | 0.50 mg/ml, 0.2 µm filtered solution in PBS |
| Stability | A minimum of 12 months when stored at ≤ -20°C as supplied | A minimum of 6 months when stored at ≤ -65°C as supplied |
| Catalog # | BT-015-GMP | BT-015-GMP/LQ |
| Pack Size | 25 µg, 1 mg | 25 µg |
Primary human T cell expansion was evaluated using both liquid format and lyophilized cytokines. Cells were expanded in G-Rex® 6M well plates using complete GMP Human T Cell Expansion Media supplemented with either 200 IU/mL GMP IL-2 or a combination of 10 ng/mL of both GMP IL-7 and GMP IL-15 in both liquid and lyophilized formats. After 9 days of expansion, cells were assessed for fold expansion and viability (left; data labels are mean % viability) as well as for memory phenotype using flow cytometry (right).
Direct comparisons of the liquid frozen (turquoise circles) and lyophilized (orange squares) formulations of GMP IL-2, IL-7, and IL-15. All 3 cytokines were tested in cell proliferation assays with no difference in bioactivity observed in any of the cytokines. IL-2 was tested on CTLL-2 mouse cytotoxic T cells, IL-7 was tested on PHA-activated human peripheral blood lymphocytes, and IL-15 was tested on MO7e human megakaryocytic leukemic cell.
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Testimonials
- Michael Paglia
Chief Operating Officer
Elevate Bio, BaseCamp