Workflow

The complete ScaleReady workflow: how everything fits into one scalable process

Built around the breakthrough advantages of G-Rex® closed-system bioreactors, the ScaleReady workflow was created specifically to produce immune cells such as T cells, natural killer (NK) cells, and hematopoietic cells. True linear scalability means that the same protocols established during research will work during large-scale production.

Wash & Prep

The Lovo® Cell Processing System is the easy to use, easy to scale instrument for larger volume applications. For small volume washing, the versatile Cue Cell Processing System handles cryopreservation preparation, electroporation preparation, and small-volume culture media exchange with precision.

Cell Selection

The ScaleReady partners are hard at work on practical, scalable products for automated cell selection. With our combined expertise, we are designing a thoughtful and intelligent cell selection solution that fits in with and enhances our workflow.

Stay tuned as we make cell selection announcements!

Cell Activation & Expansion

G-Rex closed-system bioreactors are the only system that scales up from 10 million to over 100 billion cells without gene profile changes, and scale out with one bioreactor per patient. Within a single G-Rex bioreactor cells can be activated, transduced, and expanded, dramatically simplifying manufacturing processes. Combine G-Rex with high-quality GMP media, activation reagents and cytokines sourced from Bio-Techne.

Gene Editing

The TcBusterTM non-viral gene delivery platform makes gene modification more consistent, faster, and cost-efficient, without the challenges involved in manufacturing lentiviral vectors.

Culture Harvest

The GatheRex liquid handling pump is thoughtfully designed to work with any G-Rex bioreactor for simple volume reduction and cell harvesting. The Lovo cell processing system for larger volumes and the Cue cell processing system for smaller volumes enable efficient cell washing and product concentration.

Fill & Finish

The Cue cell processing system completes the workflow for final product volumes of 10mL or smaller. It’s exacting and responsive with on-instrument wash and concentration steps, temperature- controlled automated mixing, and high-throughput fill and finish.

Get in touch to begin scaling up and scaling out.

The LOVO and Cue Cell Processing Systems are for laboratory use only. Unless the user has obtained advance clearance or approval from the appropriate regulatory agency, cells processed on this system are not intended for diagnostic purposes, direct transfusion, or for use in the production of therapeutic products or vaccines for clinical use. For applications requiring regulatory clearance or approval, users may request the required LOVO or Cue technical documentation from Fresenius Kabi to support their submissions. Refer to the LOVO Cell Processing System Operator’s Manual and Cue Cell Processing System User’s Guide for a complete list of warnings and precautions associated with the use of these products. The Cue Cell Processing System, with use of Research Use Only (RUO) disposable product, is not for human use. Cells processed using the RUO set are not for production of a therapeutic product or vaccine, clinical use, or direct transfusion to a patient. The sterility of the RUO set pathway cannot be guaranteed.