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This study introduces Cas9-CLIPT, a method to efficiently integrate large transgenes (2-6 kb) into human T cells without restriction enzymes. It produces highly potent GD2 TRAC-CAR T cells with an enriched stem cell memory phenotype. G-Rex® 6M well plates are referenced for cell cultivation post-electroporation.

This study describes 'metabolic priming' of GD2 TRAC-CAR T cells during manufacturing by activating them in glucose/glutamine-low medium and expanding in glucose/glutamine-high medium. This process enhances stem-like memory phenotypes and in vivo persistence. G-Rex®6M plates are referenced for ex vivo cell expansion.

This study demonstrates that closed-system G-Rex® bioreactors combined with Thermo Scientific Vios iDx CR CO2 incubators significantly reduce contamination risks. G-Rex® bioreactors eliminate manual handling, feature a validated sterile fluid path, and expand cells safely in low-grade cleanrooms.

This study shows that treating CRISPR-engineered GD2 TRAC-CAR T cells with the class I HDAC inhibitor chidamide enhances their stem cell memory phenotype (CD62L+/CCR7+/IL-7Rα+) and in vivo persistence without increasing exhaustion. Technical support on the G-Rex® system from ScaleReady is acknowledged.

This study outlines a rapid 3-day manufacturing method to generate CD19-CD20-CD22 trispecific CAR T-cells from whole blood. The process maintains high cell viability, transduction efficiency, and therapeutic potency. A G-Rex® 10M vessel is explicitly used for the brief T-cell culture and expansion phase.

This study details an integrated, closed CAR T-cell manufacturing workflow. The process utilizes a G-Rex® 100M-CS bioreactor for direct in-vessel CD4+/CD8+ magnetic enrichment using a prototype SepaRex base, static retroviral transduction, and T-cell expansion, eliminating separate selection or spinoculation devices.

This document provides supplemental data for the SMART culture vessel study. It details chemical solvents, concentrations, liposome additions, and experimental controls like phosphate-buffered saline. G-Rex® is not explicitly referenced in this supplemental document.

This article details a single-use metabolite absorbing resonant transducer (SMART) for continuous cell culture monitoring. Polyacrylate films absorb secreted metabolites, altering sensor permittivity. G-Rex®10 static culture vessels are explicitly referenced for validating sensor sensitivity to live cells.

This paper proposes a low-cost, portable readout system to wirelessly interrogate LC resonant sensors. Using coherent demodulation and a direct digital synthesizer, the system successfully tracks temperature changes in water. G-Rex® is not referenced in this document.

This review discusses contact-free, passive electromagnetic resonant sensors for biomedical applications. It highlights challenges like environmental noise and positional sensitivity, proposing differential sensors to increase specificity. G-Rex® is not referenced in this document.

This document provides supplemental data on the SMART sensor in G-Rex® devices. It evaluates sensor responses to T cell-secreted metabolites, kerf width effects, and reader hardware temperature impact. G-Rex® 100M is explicitly referenced as the cell culture vessel used for these tests.

This article describes integrating a SMART sensor into the G-Rex® bioreactor for real-time, noninvasive monitoring of cell expansion. G-Rex® devices, including the G-Rex® 100M and 500M, are explicitly referenced as the core manufacturing platform for scaling CAR-T cell production.