Publications
High-dimensional temporal mapping of CAR T cells reveals phenotypic and functional remodeling during manufacturing
This study profiles CAR T cells across manufacturing. Transductions and expansion were carried out in G-Rex 6M vessels to support high-density cell growth.
Publications
Extracellular viral microRNAs as biomarkers of virus infection in human cells
The study identifies viral exmiRs as markers for live virus infection in T cells. Purified T cells were activated and cultured in a G-Rex 24-well plate.
Publications
AI-assisted quantification of tumor-infiltrating lymphocytes in colorectal cancer liver metastasis
This study develops AI models to quantify TILs in colorectal cancer liver metastasis. In the Rapid Expansion Protocol (REP) stage, TILs were cultured in G-Rex 100 M.
Publications
Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances
The study reports the optimized GMP-grade manufacturing of TranspoCART19 cells. Following electroporation, cells were expanded for 11 days in G-Rex 100M devices, achieving high expansion and functionality.
Publications
Cost-effective strategies for CAR-T cell therapy manufacturing
This review discusses cost-effective CAR-T manufacturing. It highlights G-Rex technology as a main point-of-care (POC) manufacturing platform that enables high cell density production at lower costs compared to fully automated systems.
Publications
Accelerating CAR T cell manufacturing with an automated next-day process
The study presents a 24-hour CAR-T manufacturing workflow. T cell cultures were maintained in G-Rex vessels during isolation and transduction, and control 7-day expansions were also performed in G-Rex vessels.
Publications
Detailed analysis of the effects of a NOT gate on activation and growth of T cells
Tmod cells maintain robust potency and selectivity. Transduced cells were cultured in G-Rex 24 well plates, and T cells for in vivo studies were cultured in G-Rex 6 well plates.
Publications
Preclinical Development of T Cells Engineered to Express a T-Cell Antigen Coupler Targeting Claudin 18.2–Positive Solid Tumors
The study describes the preclinical evaluation of TAC01-CLDN18.2 T cells. These cells were manufactured using purified CD4/CD8 T cells in a 9-day process utilizing either the G-Rex system from Wilson Wolf or the Cocoon platform.
Publications
Low-dose targeted radionuclide therapy synergizes with CAR T cells and enhances tumor response
This study explores combining CAR T cells with 177Lu-DOTATATE targeted radionuclide therapy. CAR T cells were transduced and subsequently expanded in G-Rex 6M well plates.
Publications
A Novel B7-H4xCD3 Bispecific T Cell Engager (PF-07260437) Synergizes with Breast Cancer Standard of Care and Immune-Checkpoint Therapies
PF-07260437 demonstrates potent antitumor efficacy in breast cancer models. For adoptive T cell transfer studies, human T cells were expanded in a G-Rex cell culture device.
Publications
Scalable process development of NK and CAR-NK expansion in a closed bioreactor
The authors developed a scalable process for expanding NK and CAR-NK cells using G-Rex 100M closed-system. The study showed that cells expanded in G-Rex 100M retained similar phenotypes and cytotoxicity to those grown in G-Rex 6-well plates.
Publications
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair
This paper reports a method leveraging HMEJ for Cas9-induced targeted integration of large DNA payloads. In the lentiviral transduction comparison arm, T cells were activated and transferred to a G-Rex 24-well plate for expansion.