Publications
In Vitro Evaluation of Genetically Unmodified Ligand-Armed Allogeneic Natural Killer Cells to Treat EGFR-Positive Glioblastoma
The study evaluates Pin-EGFR-armed eNK cells. CD3-negative cells were seeded into G-Rex 6M devices for expansion and transferred to 100 M G-Rex on day 7.
Publications
Generation of T cell responses against broad KRAS hotspot neoantigens for cell therapy or TCR discovery
The NEO-STIM process generates high-frequency T cell responses to KRAS neoantigens. The protocol utilizes G-Rex 24 Well Plates, G-Rex 6 Well Plates, and G-Rex 100M/500M closed-system vessels for cell culture and expansion.
Publications
Extracellular viral microRNAs as biomarkers of virus infection in human cells
The study identifies viral exmiRs as markers for live virus infection in T cells. Purified T cells were activated and cultured in a G-Rex 24-well plate.
Publications
High-dimensional temporal mapping of CAR T cells reveals phenotypic and functional remodeling during manufacturing
This study profiles CAR T cells across manufacturing. Transductions and expansion were carried out in G-Rex 6M vessels to support high-density cell growth.
Publications
Efficient and rapid GMP-scalable production of CAR-T and CIK cells
The authors developed an efficient protocol for expanding CAR-T and CIK cells using G-Rex 24 and G-Rex 6M devices. The study highlights the scalability and high cell yield obtained using these gas-permeable culture systems.
Publications
Generation and GMP scale-up of human CAR-T cells using non-viral Sleeping Beauty transposons for B cell malignances
The study reports the optimized GMP-grade manufacturing of TranspoCART19 cells. Following electroporation, cells were expanded for 11 days in G-Rex 100M devices, achieving high expansion and functionality.
Publications
AI-assisted quantification of tumor-infiltrating lymphocytes in colorectal cancer liver metastasis
This study develops AI models to quantify TILs in colorectal cancer liver metastasis. In the Rapid Expansion Protocol (REP) stage, TILs were cultured in G-Rex 100 M.
Publications
Detailed analysis of the effects of a NOT gate on activation and growth of T cells
Tmod cells maintain robust potency and selectivity. Transduced cells were cultured in G-Rex 24 well plates, and T cells for in vivo studies were cultured in G-Rex 6 well plates.
Publications
Accelerating CAR T cell manufacturing with an automated next-day process
The study presents a 24-hour CAR-T manufacturing workflow. T cell cultures were maintained in G-Rex vessels during isolation and transduction, and control 7-day expansions were also performed in G-Rex vessels.
Publications
Cost-effective strategies for CAR-T cell therapy manufacturing
This review discusses cost-effective CAR-T manufacturing. It highlights G-Rex technology as a main point-of-care (POC) manufacturing platform that enables high cell density production at lower costs compared to fully automated systems.
Publications
A Novel B7-H4xCD3 Bispecific T Cell Engager (PF-07260437) Synergizes with Breast Cancer Standard of Care and Immune-Checkpoint Therapies
PF-07260437 demonstrates potent antitumor efficacy in breast cancer models. For adoptive T cell transfer studies, human T cells were expanded in a G-Rex cell culture device.
Publications
Low-dose targeted radionuclide therapy synergizes with CAR T cells and enhances tumor response
This study explores combining CAR T cells with 177Lu-DOTATATE targeted radionuclide therapy. CAR T cells were transduced and subsequently expanded in G-Rex 6M well plates.