Natural Killer (NK) cells play an essential role in adaptive and innate immune responses, and due to their intrinsic and non-specific anti-tumor activity, human NK cells have been employed as anti-cancer therapies. Robust expansion and maintenance of NK Cells are critical to facilitate NK cell therapy.
ExCellerate Human NK Cell Expansion Media is animal-component free (RUO) available in 1L bottles. Development of the biosimilar GMP Media in bags and bottles is in production.
Across a sampling of donors, it can be seen that non-viral TcBuster gene-edited NK cells show comparative fold expansion and growth against non-edited. Cumulative expansion outperforms non-edited cells in most cases. CAR expression also yields high quality and quantity when pairing TcBuster with ExCellerate NK Media.
ExCellerate Human NK Cell Expansion Media is optimized for NK cell expansion when used in combination with Recombinant Human Cytokines from Bio-Techne R&D Systems; however, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-15, IL-12, IL-18, and IL-21) and cell activation methods, including particle, bead-based, or plate-bound Human NKp46/NCR1 Antibody.
The media supports both feeder-based and feeder-free expansion methods.
