Generation of non-human primate CAR Tregs using artificial antigen-presenting cells, simian tropic lentiviral vectors, and antigen-specific restimulation
This protocol details the manufacturing of CAR Tregs for non-human primates. G-Rex 6-well plates were used to expand K562 artificial antigen-presenting cells (aAPCs), which were subsequently used to stimulate Tregs. The method yields highly suppressive CAR Tregs for pre-clinical studies.
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