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The study uses 3D ovarian cancer models (spheroids, collagen gels, microfluidics) to study MUC1/TnMUC1 CAR T-cell activity. Resistance mechanisms and fibroblast interactions are explored. Transduced CAR T cells were cultured and expanded in G-Rex plates for 10 days.

This study uses Quality-by-Design (QbD) and Design of Experiments (DOE) to optimize CAR-T expansion. Small-scale DOE studies were conducted in G-Rex 24-well plates to identify optimal parameters (single activation, short seed train), which were then translated to Ambr 250 bioreactors.

The study analyzes T-Ag-reactive CD8+ T cells in Merkel cell carcinoma patients treated with anti-PD1. Responders showed broader T-cell recognition. T-Ag-specific T cells were expanded from PBMCs using artificial Ag-presenting scaffolds in G-Rex 24 well plates to enhance tumor killing.

The study describes a mesothelin-specific HLA-independent TCR (HiT) that activates T cells and kills tumor cells in vitro and in vivo. Large-scale T-cell transduction and expansion were performed using G-Rex 6M plates.

The study develops allogeneic "Smart CAR T cells" using TALEN gene editing. These cells constitutively express a FAP CAR to target CAFs, which induces mesothelin CAR expression. This logic-gated approach improves solid tumor efficacy and safety. G-Rex 6M plates were used for the expansion of gene-edited T cells.

A case report detailing the treatment of refractory DLBCL using CIK cells combined with obinutuzumab. The therapy achieved a very good partial remission without severe toxicity. CIK cells were produced and expanded for 14 days in G-Rex using a blinatumomab-based protocol.

This study demonstrates that human keratinocytes cultured on a gas-permeable interface (GPI) stratify to form a mature epidermis, comparable to the air-liquid interface method. G-Rex 6 well plates were used as the GPI device for culturing the keratinocytes and inducing stratification.

This study investigates the specificity of the MR1-restricted TCR from clone MC.7.G5. Results show recognition is restricted to the MR1*04 allomorph and is not cancer-specific, reacting to healthy cells expressing MR1*04. G-Rex systems were used to seed and expand CD3+ T cells during the transduction process.

This study assesses TIL expansion from HNSCC samples, finding sTIL percentage as a key predictor. A subset of samples underwent a Rapid Expansion Protocol (REP) using either T-flasks or G-Rex vessels, achieving high fold expansion.

This study demonstrates that the 4-1BBζ costimulatory domain enhances CD8+ T-cell proliferation and cytokine production following TCR stimulation compared to CD28ζ. CAR T-cells were manufactured and expanded in G-Rex plates for 13 days prior to functional analysis.

This study shows that modulating glucose/glutamine during activation and expansion (metabolic priming) improves TRAC-CAR T cell stemness and in vivo persistence. Expansion of the gene-edited cells was performed in G-Rex 6M plates.

The study demonstrates that HSPC-derived CAR-T cells exhibit superior persistence and viral control compared to adoptively transferred T cells in HIV models. The adoptive CAR-T cell products were manufactured and expanded for ~7 days in G-Rex.