Publications
EphA3 CAR T cells are effective against glioblastoma in preclinical models
The study investigates EphA3-targeted CAR T cells for glioblastoma. T cells were transduced and expanded in G-Rex 24 well plates.
Publications
Engineering memory T cells as a platform for long-term enzyme replacement therapy in lysosomal storage disorders
This study engineers memory T cells to secrete IDUA for treating MPS I. Engineered Tm cells were cultured and expanded in 24-well G-Rex tissue culture plates.
Publications
In silico and pharmacological evaluation of GPR65 as a cancer immunotherapy target regulating T-cell functions
The study evaluates GPR65 as a target. TCR-T cells (NY-ESO1 or MAGE-A4) were produced in G-Rex 6 Well Plate systems.
Publications
synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy
The study develops synNotch-programmed iPSC-NK cells to target CD155 and CD73 in glioblastoma. Cells were expanded for 2-3 weeks in G-Rex 24 well plates using NK Xpander medium.
Publications
Building a Better Defense: Expanding and Improving Natural Killer Cells for Adoptive Cell Therapy
A review on NK cell expansion and improvement. It mentions G-Rex plates as a culture material used in protocols for expanding NK cells, specifically citing studies where G-Rex 6-well plates or G-Rex 100M vessels were used.
Publications
Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes
The study introduces "NeoExpand" to selectively expand neoantigen-reactive TILs. It compares this to rapid expansion protocols (REP). G-Rex 24-well, 6-well, and 100 were used for the rapid expansion of T cells.
Publications
Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method
The paper describes a feeder-free method to generate AlloCAR-NKT cells from HSPCs. G-Rex 6M well plates were used for the expansion stage (stage 4) of the NKT cell culture.
Publications
Expansion and Precise CRISPR-Cas9 Gene Repair of Autologous T-Memory Stem Cells from Patients with T-Cell Immunodeficiencies
This protocol details the expansion and gene repair of TSCM cells. It mentions that for scale-up and GMP-compliant experiments, electroporated TSCM can be expanded in G-Rex 100M gas-permeable culture vessels.
Publications
Virus-Specific T-Cell Therapy for the Management of Viral Infections in the Immunocompromised
A review article discussing VST therapies for immunocompromised patients. It mentions that simplified manufacturing processes use G-Rex culture devices for the selective activation and expansion of virus-reactive T cells.
Publications
Inosine Induces Stemness Features in CAR-T cells and Enhances Potency
The study shows that inosine induces stemness and metabolic reprogramming in CAR-T cells, improving potency. Clinical-scale manufacturing of GD2-CAR-T cells in inosine-containing media was performed using a semi-closed G-Rex system.
Publications
Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay
This paper evaluates a flow cytometric cytotoxicity assay for multiVSTs. It highlights that multiVSTs, expanded in G-Rex 5M devices for 14 days, show potent cytolytic activity against viral targets that is better detected by this new assay than by chromium release.
Publications
Antiviral cellular therapy for enhancing T-cell reconstitution before or after hematopoietic stem cell transplantation (ACES): a two-arm, open label phase II interventional trial of pediatric patients with risk factor assessment
A Phase II trial evaluating third-party VSTs for refractory viral infections in pediatric patients post-HSCT or with IEI. VSTs were expanded using a rapid expansion protocol in G-Rex-10 for 10 days. Clinical response rate was 62%.