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This study evaluates shipping human islets in high density using silicone rubber membrane (SRM) vessels (G-Rex). The method maintained islet viability and function comparable to low-density controls, allowing an entire clinical islet isolation to be shipped in a single vessel.

This dissertation analyzes human Treg subsets and their TCR repertoires. It demonstrates that expanding Tregs in G-Rex10 devices with rapamycin enhances expansion (at least 50-fold) compared to plates, while maintaining FoxP3 expression and suppressive function, facilitating clinical translation.

This is the published version of the study optimizing TIL propagation using K562 aAPCs. It confirms that the aAPC/G-Rex platform supports rapid expansion of TILs to clinical scales with an effector-memory phenotype, comparable to PBMC feeders. Note: This is a duplicate study of 2014_Forgetet_al_TIL.pdf.

This study defines optimized parameters for the G-Rex 'M' series using K562 cells. It demonstrates that 1L of medium with optimal seeding allows 100-fold expansion in 10 days in the G-Rex 100M. The platform showed linear scalability from 5 to 500 cm2 and validated glucose measurement for cell quantification.

This study reports the expansion of HSPCs using Tat-MYC and Tat-Bcl-2 fusion proteins. G-Rex 10 devices were used to expand CD34+ cells from cord blood, mobilized blood, and bone marrow. The expanded cells retained long-term repopulating activity and differentiated into multiple lineages in vivo.

This conference paper reports a method for large-scale generation of myeloid dendritic cells (DCs) from cord blood CD34+ cells using G-Rex10. The G-Rex system yielded over 1.5 billion DCs, significantly outperforming conventional culture plates, and produced DCs capable of inducing Th1 responses.

This research investigates the kinetics of tumor destruction by CAR T cells targeting MUC1 and PSCA. G-Rex 100M devices were used to expand the CAR-modified T cells. The study found that dual targeting improved efficacy but antigen intensity influenced killing; epigenetic modulation enhanced tumor susceptibility.

This study utilized G-Rex to expand NK cells from cord blood using artificial antigen presenting cells (aAPCs). The protocol achieved a mean 2389-fold expansion of high-purity NK cells in 14 days. Expanded cells demonstrated significant in vitro and in vivo cytotoxicity against multiple myeloma.

This study demonstrates the generation of T-cell lines targeting WT1, Survivin, MAGE-A3, and PRAME from pediatric ALL patients using autologous DCs and PHA-blasts. The cells showed specific anti-leukemic activity. G-Rex are referenced as a strategy for scaling up to clinical cell numbers.

This study demonstrates that nicotinamide (NAM) enhances the homing and retention of NK cells in bone marrow, spleen, and lung. NK cells were expanded in G-Rex devices under feeder-free conditions with IL-2 or IL-15. The G-Rex platform supported large-scale expansion, yielding high-purity NK cells.

This study reports a simplified method to generate T cells targeting EBV, CMV, and Adenovirus using nucleofected dendritic cells and G-Rex devices. The 10-day G-Rex protocol produced clinically relevant cell numbers. Infused cells were safe and effective against active viral infections in HSCT recipients.

Compares unselected young TILs vs CD8+-enriched TILs. Mentions that G-Rex were optimized for TIL therapy and introduced for clinical use. The study used (WAVE and G-Rex) for high-density T-cell expansion, facilitating rapid accrual