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The study demonstrates a method to enrich gene-edited CD4+ T cells using methotrexate selection. G-Rex 10 were employed for the long-term expansion of lentivirus-modified T cells, facilitating the generation of large numbers of selected cells.

The study explores antibody responses from tumor-infiltrating B cells against viral antigens in cancer patients. G-Rex were used to expand tumor-infiltrating lymphocytes (TILs) from tumor tissue, facilitating the analysis of immune reactivity in the tumor microenvironment.

This paper describes an improved method for generating CAR-T cells using the piggyBac transposon system. G-Rex10 culture were used for the expansion phase, contributing to the generation of clinically relevant numbers of CAR-T cells with a memory phenotype.

This study demonstrates a method for generating tumor-specific TCR-T cells using CRISPR/Cas9 for endogenous TCR knockout and AAV for TCR insertion. G-Rex 24-well plates were utilized for T cell expansion, supporting cell viability and growth during the editing process.

This Phase I trial assessed the safety of infusing autologous expanded Tregs into kidney transplant recipients to induce tolerance. G-Rex were used to expand Tregs, achieving robust cell numbers and meeting all clinical release criteria without adverse events.

A method to generate virus-reactive T cells from cord blood using accelerated co-cultured dendritic cells (acDC). The use of G-Rex 10 significantly increased the yield of antigen-reactive CD8+ T cells compared to standard plates, enabling therapeutic dosing.

Reviews improvements to TIL manufacturing. Implementing G-Rex 100MCS devices for the Rapid Expansion Protocol (REP) reduced technical interventions, media usage, and contamination risk while maintaining comparable tumor specificity to flask/bag methods.

Evaluated a closed-system manufacturing process for TCR-transduced T-cells. The rapid expansion phase utilized closed G-Rex 500CS devices, yielding ~20 x 10^9 highly functional T-cells from a single vessel, improving safety and consistency.

Developed "universal" CAR T cells using a lentiviral vector coupling CAR expression with CRISPR/Cas9 gene editing. G-Rex were used for conventional control cultures to compare against the automated CliniMACS Prodigy system.

A scalable protocol for producing red blood cells from adult PBMCs was developed. The G-Rex allowed for massive expansion (3x10^6 fold) of erythroblasts and supported efficient differentiation and enucleation, comparable to standard culture dishes.

Review comparing cell culture systems (bags vs flasks) for dendritic cell production. Discusses gas permeability and materials. References Wilson Wolf patents related to gas-permeable devices as examples of improved culture methods utilizing silicone rubber membranes.

Prospective analysis of TIL therapy in metastatic melanoma. The Rapid Expansion Protocol (REP) was performed using G-Rex 100M, contributing to the generation of infusion products. High TIL numbers were associated with response.