Publications
Building a Better Defense: Expanding and Improving Natural Killer Cells for Adoptive Cell Therapy
A review on NK cell expansion and improvement. It mentions G-Rex plates as a culture material used in protocols for expanding NK cells, specifically citing studies where G-Rex 6-well plates or G-Rex 100M vessels were used.
Publications
synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma therapy
The study develops synNotch-programmed iPSC-NK cells to target CD155 and CD73 in glioblastoma. Cells were expanded for 2-3 weeks in G-Rex 24 well plates using NK Xpander medium.
Publications
Expansion and Precise CRISPR-Cas9 Gene Repair of Autologous T-Memory Stem Cells from Patients with T-Cell Immunodeficiencies
This protocol details the expansion and gene repair of TSCM cells. It mentions that for scale-up and GMP-compliant experiments, electroporated TSCM can be expanded in G-Rex 100M gas-permeable culture vessels.
Publications
Generation of allogeneic CAR-NKT cells from hematopoietic stem and progenitor cells using a clinically guided culture method
The paper describes a feeder-free method to generate AlloCAR-NKT cells from HSPCs. G-Rex 6M well plates were used for the expansion stage (stage 4) of the NKT cell culture.
Publications
Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes
The study introduces "NeoExpand" to selectively expand neoantigen-reactive TILs. It compares this to rapid expansion protocols (REP). G-Rex 24-well, 6-well, and 100 were used for the rapid expansion of T cells.
Publications
Virus-Specific T-Cell Therapy for the Management of Viral Infections in the Immunocompromised
A review article discussing VST therapies for immunocompromised patients. It mentions that simplified manufacturing processes use G-Rex culture devices for the selective activation and expansion of virus-reactive T cells.
Publications
Assessment of the cytolytic potential of a multivirus-targeted T cell therapy using a vital dye-based, flow cytometric assay
This paper evaluates a flow cytometric cytotoxicity assay for multiVSTs. It highlights that multiVSTs, expanded in G-Rex 5M devices for 14 days, show potent cytolytic activity against viral targets that is better detected by this new assay than by chromium release.
Publications
Inosine Induces Stemness Features in CAR-T cells and Enhances Potency
The study shows that inosine induces stemness and metabolic reprogramming in CAR-T cells, improving potency. Clinical-scale manufacturing of GD2-CAR-T cells in inosine-containing media was performed using a semi-closed G-Rex system.
Publications
Antiviral cellular therapy for enhancing T-cell reconstitution before or after hematopoietic stem cell transplantation (ACES): a two-arm, open label phase II interventional trial of pediatric patients with risk factor assessment
A Phase II trial evaluating third-party VSTs for refractory viral infections in pediatric patients post-HSCT or with IEI. VSTs were expanded using a rapid expansion protocol in G-Rex-10 for 10 days. Clinical response rate was 62%.
Publications
Human 3D Ovarian Cancer Models Reveal Malignant Cell–Intrinsic and –Extrinsic Factors That Influence CAR T-cell Activity
The study uses 3D ovarian cancer models (spheroids, collagen gels, microfluidics) to study MUC1/TnMUC1 CAR T-cell activity. Resistance mechanisms and fibroblast interactions are explored. Transduced CAR T cells were cultured and expanded in G-Rex plates for 10 days.
Publications
A quality-by-design approach to improve process understanding and optimise the production and quality of CAR-T cells in automated stirred-tank bioreactors
This study uses Quality-by-Design (QbD) and Design of Experiments (DOE) to optimize CAR-T expansion. Small-scale DOE studies were conducted in G-Rex 24-well plates to identify optimal parameters (single activation, short seed train), which were then translated to Ambr 250 bioreactors.
Publications
Selection, engineering, and in vivo testing of a human leukocyte antigen–independent T-cell receptor recognizing human mesothelin
The study describes a mesothelin-specific HLA-independent TCR (HiT) that activates T cells and kills tumor cells in vitro and in vivo. Large-scale T-cell transduction and expansion were performed using G-Rex 6M plates.