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Knockout of DNMT3A in CAR-T cells prevents exhaustion and enhances antitumor activity. G-Rex-6M culture plates were used for the expansion of gene-edited CAR-T cells. The modified cells retained a stem-like epigenetic program and showed superior tumor control in vivo.

The authors investigate MAGE-A3 as a therapeutic target, proposing EPS8L2 as the source of prior TCR-T neurotoxicity. They developed MAGE-A3-directed CARs and TCRs, expanding transduced primary T cells in G-Rex plates. The CARs showed improved selectivity against the off-target peptide.

This study evaluates 89Zr-Df-IAB22M2C PET imaging to track CD8+ T cells in a colorectal cancer model treated with a GUCY2C-CD3 bispecific antibody. G-Rex devices were used to expand human T cells prior to adoptive transfer. The tracer effectively monitored dose-dependent T cell infiltration.

The study presents a microfluidic device (VECT) for mRNA delivery into primary T cells, NK cells, and HSPCs. G-Rex plates were used for the culture of NK cells during the expansion phase. The method achieved high transfection efficiency and viability without compromising cell function.

RNA-seq revealed that ex vivo expansion alters chemokine receptor expression in NK cells, notably upregulating CCR5. G-Rex were used to expand NK cells with feeder cells. CRISPR-mediated CCR5 disruption reduced liver trafficking and increased NK cell presence in circulation in mice.

A high-throughput assay identified optimal cytokine combinations for viral-specific T cell (VST) expansion. The study validated that IL15/IL6 culture conditions in G-Rex10 gas-permeable devices produced VSTs functionally equivalent to standard IL4/IL7 conditions. This method streamlines process development.

The authors generated "Cerberus" T cells (Cb-STs) targeting multiple viruses and Aspergillus fumigatus, with CRISPR/Cas9-mediated glucocorticoid receptor disruption. G-Rex10 devices were used for the culture and expansion of these multi-pathogen specific T cells. Cb-STs maintained function in the presence of dexamethasone.

This study repurposes non-transplantable umbilical cord blood units to generate myeloid dendritic cells (DCs) and bivalent-leukemia-specific T cells. G-Rex were used to expand DCs from CD34+ cells, facilitating scalable production. The generated T cells showed specificity against WT1 and PRAME.

The study presents a manufacturing platform for CD19-specific CAR T cells using enzyme-produced piggyBac transposon DNA and mRNA transposase. G-Rex10 were utilized for large-scale expansion, yielding up to 10^8 CAR-T cells per electroporation. The method allows precise control of vector copy number.

This research optimizes cryopreservation strategies for regulatory T cell (Treg) products. G-Rex were used for the culture and expansion of Treg cells and for transporting fresh nTreg products. Results show that a freezing medium with 5% DMSO significantly improves cell viability and recovery.

This study evaluates differentiating NK cells from iPSCs under chemically defined, serum-free conditions without feeder layers. G-Rex plates were used to expand hiPSC01-derived NK cells and peripheral blood NK cells for 2 weeks, achieving high fold expansion. The method yields functional, cytotoxic NK cells.

This paper investigates the role of p16INK4a in T cell senescence. G-Rex and G-Rex 24-well plates were used to culture T cells for polyclonal activation and antigen-specific line generation, facilitating the study of long-term dysfunction.