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This study develops a protocol for manufacturing base-edited CAR-T cells using circular RNA (circRNA) to reduce costs and improve stability compared to linear mRNA. Large-scale production of PD-1 deficient CAR-T cells was achieved using 100M and six-well G-Rex vessels, demonstrating scalability.

This phase I trial evaluates locoregional B7-H3 CAR-T cells for DIPG. The clinical CAR-T cell products were manufactured using G-Rex 100MCS vessels, incorporating a methotrexate selection step to enrich for modified cells.

The study reports on the IMA101 clinical trial. Endogenous multi-target T cells were primed and expanded using G-Rex 100MCS and G-Rex 100M vessels in a rapid expansion protocol, enabling the generation of large cell numbers for patient infusion.

This study develops Dox-inducible lentiviral vectors for CAR-T cells. G-Rex plates were used for the production of monoclonal antibodies from hybridomas and for expanding transduced primary T cells in the absence of Dox prior to in vivo experiments.

The paper describes a manufacturing strategy for Aspergillus-specific T cells (ATCs). A short-term expansion (STE) protocol using G-Rex devices allowed for the generation of clinically relevant numbers of functional ATCs within 12 days.

The study investigates T cell responses to SARS-CoV-2 and endemic coronaviruses. It describes a protocol to generate universal multi-coronavirus specific T cells (MCVSTs) by expanding PBMCs in G-Rex gas-permeable containers, yielding robust cell numbers for potential adoptive immunotherapy.

This paper describes a pooled screening strategy for CAR domain combinations using DNA barcoding. G-Rex plates were utilized for co-culturing transduced T cells with tumor cells during repeated antigen stimulation assays to identify CAR constructs with superior expansion and proliferation.

The study evaluates an oncolytic adenovirus expressing vIL-2 to boost TIL therapy for ovarian cancer. Autologous TILs were expanded from patient tumor fragments using six-well G-Rex plates as part of the 'young' TILs expansion protocol.

This study demonstrates that an oncolytic adenovirus encoding vIL-2 enhances NK cell cytotoxicity against ovarian cancer. Patient PBMCs used for humanized mouse models were expanded and rested using 6-well G-Rex plates with TILs media.

The study analyses neoantigen-specific T cells in melanoma patients. Isolated neoTCRs were engineered into donor T cells, which were expanded in 24-well G-Rex plates. The expanded cells were used to validate TCR specificity and cytotoxicity against autologous tumor cell lines.

The study isolates neutralizing human mAbs against Sosuga virus from a survivor. Hybridoma cell lines secreting these mAbs were expanded in 6-well G-Rex plates to produce sufficient antibodies for purification and subsequent functional characterization.

This study evaluates anti-CD20 pre-treatment for enhancing SIV-specific CAR-T cell therapy in macaques. CAR-T cells were manufactured using G-Rex 6-well plates for expansion. While anti-CD20 treatment increased initial CAR-T expansion, the cells failed to persist long-term, and viral loads were not significantly affected.